Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   CWI20_RS25595 Genome accession   NZ_CP025056
Coordinates   5478096..5478521 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PB367     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5473096..5483521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI20_RS25580 (CWI20_26155) pilX 5473660..5474247 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  CWI20_RS25585 (CWI20_26160) pilY1 5474259..5477750 (+) 3492 WP_003094704.1 type 4a pilus biogenesis protein PilY1 -
  CWI20_RS25590 (CWI20_26165) pilY2 5477752..5478099 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  CWI20_RS25595 (CWI20_26170) comF 5478096..5478521 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  CWI20_RS25600 (CWI20_26175) ispH 5478568..5479512 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  CWI20_RS25605 (CWI20_26180) fkpB 5479598..5480038 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  CWI20_RS25610 (CWI20_26185) lspA 5480031..5480540 (-) 510 WP_003094728.1 signal peptidase II -
  CWI20_RS25615 (CWI20_26190) ileS 5480533..5483364 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=217219 CWI20_RS25595 WP_003094721.1 5478096..5478521(+) (comF) [Pseudomonas aeruginosa strain PB367]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=217219 CWI20_RS25595 WP_003094721.1 5478096..5478521(+) (comF) [Pseudomonas aeruginosa strain PB367]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment