Detailed information NTG0021566

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: SPY_RS06800
• Genome accession: NC_002737
• Coordinates: 1350674..1353058 (-)
• Length: 794 a.a.
• NCBI ID: WP_010922541.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes M1 GAS
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1345674..1358058

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPY_RS06780 (SPy1625)	pknB	1345692..1347590 (-)	1899	WP_010922538.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
SPY_RS06785 (SPy1626)	-	1347587..1348327 (-)	741	WP_002983660.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
SPY_RS06790 (SPy1627)	rsmB	1348365..1349687 (-)	1323	WP_010922539.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
SPY_RS06795 (SPy1628)	fmt	1349677..1350612 (-)	936	WP_010922540.1	methionyl-tRNA formyltransferase	-
SPY_RS06800 (SPy1629)	priA	1350674..1353058 (-)	2385	WP_010922541.1	primosomal protein N'	Machinery gene
SPY_RS06805 (SPy1630)	rpoZ	1353123..1353440 (-)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
SPY_RS06810 (SPy1632)	gmk	1353456..1354091 (-)	636	WP_010922542.1	guanylate kinase	-
SPY_RS06815 (SPy1633)	-	1354201..1355808 (-)	1608	WP_002988954.1	ribonuclease Y	-
SPY_RS06820 (SPy1634)	-	1355938..1356834 (-)	897	WP_010922543.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90315.24 Da
• Isoelectric Point: 9.4042

>NTDB_id=21566 SPY_RS06800 WP_010922541.1 1350674..1353058(-) (priA) [Streptococcus pyogenes M1 GAS]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKSDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVAYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMSIRHQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=21566 SPY_RS06800 WP_010922541.1 1350674..1353058(-) (priA) [Streptococcus pyogenes M1 GAS]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTATTA
TTGGTTTTGGCCAAGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAG
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGTCTGATAGGGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGGCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTAGAGATGTGGTGGCTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCCAATT
GATCGCAGTGAGTCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAGGCGTCAGCTGTTAC
TGAAATTGTTGAACAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCGGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTGCCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATCGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGTGTCTATCATTTTATTCAATTAACGCAAAGAGCAAACCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCTTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAATAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATTCCAGAAGCTAAGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACTTCCTTAAATTTGCCGGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAGGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCTTTTTACCGTTATGAGATGTCTATTCGTCATCAAATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-535)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.46	100	0.471