Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BWZ32_RS00190 Genome accession   NZ_CP019320
Coordinates   36806..37813 (-) Length   335 a.a.
NCBI ID   WP_011151279.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV2014DJH     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 31806..42813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWZ32_RS00165 (BWZ32_00165) aroK 32623..33141 (-) 519 WP_011079357.1 shikimate kinase AroK -
  BWZ32_RS00170 (BWZ32_00170) pilQ 33343..35127 (-) 1785 WP_011079356.1 type IV pilus secretin PilQ Machinery gene
  BWZ32_RS00175 (BWZ32_00175) pilP 35141..35653 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  BWZ32_RS00180 (BWZ32_00180) pilO 35643..36233 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  BWZ32_RS00185 (BWZ32_00185) pilN 36226..36816 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  BWZ32_RS00190 (BWZ32_00190) pilM 36806..37813 (-) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  BWZ32_RS00195 (BWZ32_00195) - 37953..40466 (+) 2514 WP_011079351.1 penicillin-binding protein 1A -
  BWZ32_RS00200 (BWZ32_00200) oxyR 40537..41436 (-) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  BWZ32_RS00205 (BWZ32_00205) - 41710..41982 (+) 273 WP_101956525.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37638.05 Da        Isoelectric Point: 6.3873

>NTDB_id=214813 BWZ32_RS00190 WP_011151279.1 36806..37813(-) (pilM) [Vibrio vulnificus strain VV2014DJH]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVALAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=214813 BWZ32_RS00190 WP_011151279.1 36806..37813(-) (pilM) [Vibrio vulnificus strain VV2014DJH]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGGCACTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCGTTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGACGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.433

100

0.534
  pilM Vibrio cholerae strain A1552

49.267

100

0.501

Multiple sequence alignment