Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   ECs_4346 Genome accession   NC_002695
Coordinates   4351745..4352509 (+) Length   254 a.a.
NCBI ID   NP_312373.1    Uniprot ID   Q8X5U1
Organism   Escherichia coli O157:H7 str. Sakai     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4346745..4357509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECs_4342 acpT 4347699..4348286 (+) 588 NP_312369.1 4'-phosphopantetheinyl transferase -
  ECs_4343 nikA 4348397..4349971 (+) 1575 NP_312370.1 nickel/heme ABC transporter periplasmic binding protein -
  ECs_4344 nikB 4349971..4350915 (+) 945 NP_312371.1 nickel ABC transporter permease -
  ECs_4345 nikC 4350912..4351745 (+) 834 NP_312372.1 nickel ABC transporter permease -
  ECs_4346 amiE 4351745..4352509 (+) 765 NP_312373.1 nickel ABC transporter ATPase Regulator
  ECs_4347 nikE 4352506..4353312 (+) 807 NP_312374.1 nickel ABC transporter ATPase -
  ECs_4348 nikR 4353318..4353719 (+) 402 NP_312375.1 transcriptional repressor -
  ECs_4349 - 4353918..4354664 (+) 747 NP_312376.2 GntR family regulatory protein -
  ECs_4350 - 4354689..4355162 (+) 474 NP_312377.1 phosphotransferase system enzyme IIA -
  ECs_4351 - 4355159..4355440 (+) 282 NP_312378.1 phosphotransferase system enzyme IIB -
  ECs_4352 - 4355517..4356875 (+) 1359 NP_312379.1 phosphotransferase system enzyme IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26790.39 Da        Isoelectric Point: 6.5997

>NTDB_id=21475 ECs_4346 NP_312373.1 4351745..4352509(+) (amiE) [Escherichia coli O157:H7 str. Sakai]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=21475 ECs_4346 NP_312373.1 4351745..4352509(+) (amiE) [Escherichia coli O157:H7 str. Sakai]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTGTCGTTAACCCTGAA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGAATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAGACGTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTTTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCACGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCGCCGGGGATGCTGCTGGTCACCCATGATATGGGCGTGGTGGCGCGTCTGGCGGATGACGTAGCGGTAA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X5U1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment