Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   CR537_RS01820 Genome accession   NZ_CP024138
Coordinates   355980..356744 (-) Length   254 a.a.
NCBI ID   WP_001136206.1    Uniprot ID   -
Organism   Escherichia coli strain 14EC020     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 350980..361744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR537_RS01795 (CR537_01800) yhhJ 352822..353946 (+) 1125 WP_001318088.1 ABC transporter permease -
  CR537_RS01800 (CR537_01805) - 354019..354294 (+) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  CR537_RS01805 (CR537_01810) - 354291..354650 (+) 360 WP_000593557.1 type II toxin-antitoxin system HicB family antitoxin -
  CR537_RS01810 (CR537_01815) nikR 354770..355171 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  CR537_RS01815 (CR537_01820) nikE 355177..355983 (-) 807 WP_000173650.1 nickel import ATP-binding protein NikE -
  CR537_RS01820 (CR537_01825) amiE 355980..356744 (-) 765 WP_001136206.1 nickel import ATP-binding protein NikD Regulator
  CR537_RS01825 (CR537_01830) nikC 356744..357577 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  CR537_RS01830 (CR537_01835) nikB 357574..358518 (-) 945 WP_000947063.1 nickel ABC transporter permease subunit NikB -
  CR537_RS01835 (CR537_01840) nikA 358518..360092 (-) 1575 WP_039003979.1 nickel ABC transporter substrate-binding protein -
  CR537_RS01840 (CR537_01845) acpT 360203..360790 (-) 588 WP_000285764.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26810.47 Da        Isoelectric Point: 6.9016

>NTDB_id=210948 CR537_RS01820 WP_001136206.1 355980..356744(-) (amiE) [Escherichia coli strain 14EC020]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHVVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=210948 CR537_RS01820 WP_001136206.1 355980..356744(-) (amiE) [Escherichia coli strain 14EC020]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGCATTCTCC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
AGCGCTGGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACTACCGACCTTGATGTGGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAGAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGATGTGGCGGTGA
TGTCGCACGGTAAAATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGTGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment