Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BSL056_RS05225 Genome accession   NZ_CP018197
Coordinates   1063568..1067077 (+) Length   1169 a.a.
NCBI ID   WP_073204605.1    Uniprot ID   -
Organism   Bacillus safensis strain KCTC 12796BP     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1058568..1072077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSL056_RS05195 (BSL056_05225) lepB 1059084..1059596 (+) 513 WP_024425493.1 signal peptidase I -
  BSL056_RS05200 (BSL056_05230) - 1059635..1060780 (-) 1146 WP_024425494.1 AbrB family transcriptional regulator -
  BSL056_RS05205 (BSL056_05235) - 1061049..1061471 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  BSL056_RS05210 (BSL056_05240) - 1061464..1062303 (+) 840 WP_073204603.1 M56 family metallopeptidase -
  BSL056_RS05215 (BSL056_05245) - 1062361..1062771 (+) 411 WP_024425497.1 DoxX family protein -
  BSL056_RS05220 (BSL056_05250) - 1062969..1063415 (-) 447 WP_024425498.1 ferritin-like domain-containing protein -
  BSL056_RS05225 (BSL056_05255) addB 1063568..1067077 (+) 3510 WP_073204605.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BSL056_RS05230 (BSL056_05260) addA 1067055..1070759 (+) 3705 WP_073204608.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BSL056_RS05235 (BSL056_05265) sbcD 1070786..1071961 (+) 1176 WP_073204610.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134890.03 Da        Isoelectric Point: 5.4378

>NTDB_id=207014 BSL056_RS05225 WP_073204605.1 1063568..1067077(+) (addB) [Bacillus safensis strain KCTC 12796BP]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKLPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHEHALYVLDPEQLDDKEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=207014 BSL056_RS05225 WP_073204605.1 1063568..1067077(+) (addB) [Bacillus safensis strain KCTC 12796BP]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCAAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCTGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCCAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGTCATCTTCATACGCCGGATCTTGCTTATTTAG
AACGGCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTCACTCCTCATCTCACAGTATCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGATGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGTTTCCATTACAGGCGGT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCTCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTAGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGATAAACTTGAAAAGGCTAGGCTGGAAGCTGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTATTGTCTGAGGATGACAGGGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATGAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACAAGGAGCAATTAAAATTTGTGGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAATCAATATCCCATTAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTATGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCCTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATCCTATTAAGCTCTAAC
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGCCAAGGACCACTTCCACCGTTTACTTTTCAATTGAAAAATG
GCTGTACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGGCTGGCCTTGCAAATGCTCACATACTTAGA
TCTGACCATTACGTATTCGAAGGAGTGGCTCGGTGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAACAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAAGATGGGTCCTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.978

99.316

0.655

Multiple sequence alignment