Detailed information NTG0204332

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: BMF34_RS05930
• Genome accession: NZ_CP017952
• Coordinates: 1193842..1194705 (-)
• Length: 287 a.a.
• NCBI ID: WP_003099993.1
• Uniprot ID: A0A3L8GIC3
• Organism: Streptococcus iniae strain 89353
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1188842..1199705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BMF34_RS05910 (BMF34_05850)	-	1189096..1189476 (-)	381	WP_003099988.1	OsmC family protein	-
BMF34_RS05915 (BMF34_05855)	pheT	1189549..1191954 (-)	2406	WP_003099989.1	phenylalanine--tRNA ligase subunit beta	-
BMF34_RS05920 (BMF34_05860)	-	1191970..1192494 (-)	525	WP_003099991.1	N-acetyltransferase	-
BMF34_RS05925 (BMF34_05865)	pheS	1192507..1193550 (-)	1044	WP_003099992.1	phenylalanine--tRNA ligase subunit alpha	-
BMF34_RS05930 (BMF34_05870)	endA	1193842..1194705 (-)	864	WP_003099993.1	DNA/RNA non-specific endonuclease	Machinery gene
BMF34_RS05935 (BMF34_05875)	-	1194736..1194924 (-)	189	WP_003099995.1	DNA-directed RNA polymerase subunit beta	-
BMF34_RS05940 (BMF34_05880)	murA	1194928..1196199 (-)	1272	WP_003099997.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
BMF34_RS05945	-	1196265..1196501 (-)	237	WP_071794729.1	DUF1146 family protein	-
BMF34_RS05950 (BMF34_05885)	-	1196577..1196993 (-)	417	WP_003100001.1	F0F1 ATP synthase subunit epsilon	-
BMF34_RS05955 (BMF34_05890)	atpD	1197006..1198412 (-)	1407	WP_003100002.1	F0F1 ATP synthase subunit beta	-
BMF34_RS05960 (BMF34_05895)	-	1198528..1199403 (-)	876	WP_003100004.1	F0F1 ATP synthase subunit gamma	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 31715.35 Da
• Isoelectric Point: 9.3865

>NTDB_id=204332 BMF34_RS05930 WP_003099993.1 1193842..1194705(-) (endA) [Streptococcus iniae strain 89353]
MSPKTKQSQKINLLLFLLVLVGLFYLIDSKEGEENNPIRAFYQSVMGASNQTSQKESSNPDTPSYALASSVLTEKVKRQL
GSKVEWNGNGAFVINDNSTNLDAKVSSAPYAHNQTRLLSGKKLPTVANALLAKSTRQYRDRNETGNGYSYWKPAGWHQIH
GLSGKYDHAVDRGHLLGYALVGGLKGFDASTSNSDNIATQLSWANQAMDSDSTGQNYYETKIRRALDKNKRVRYRVTLIY
EADNILASGSHLEAKSDDGSLEFNVFVPNVQKGLEVDYQTGQITVSH

Nucleotide

• Length: 864 bp

>NTDB_id=204332 BMF34_RS05930 WP_003099993.1 1193842..1194705(-) (endA) [Streptococcus iniae strain 89353]
ATGTCACCAAAGACAAAACAAAGTCAAAAAATCAATCTATTATTGTTTCTGCTTGTGCTCGTTGGCTTATTCTATTTGAT
TGATAGTAAAGAAGGGGAAGAGAATAATCCTATTCGAGCCTTTTATCAATCAGTGATGGGAGCTTCCAATCAAACTAGCC
AAAAAGAATCAAGCAATCCTGATACACCAAGTTATGCCTTGGCATCTTCGGTTTTAACAGAAAAAGTAAAAAGACAACTT
GGTTCGAAAGTTGAGTGGAATGGTAATGGTGCATTTGTCATCAATGATAACAGTACCAATTTGGATGCAAAAGTATCCAG
TGCTCCCTATGCTCACAATCAAACCAGACTCTTATCAGGGAAAAAGCTTCCAACAGTCGCCAATGCTTTACTTGCCAAAT
CAACAAGGCAATACCGAGACCGAAACGAGACCGGAAATGGCTACTCTTATTGGAAACCTGCTGGTTGGCACCAGATTCAT
GGTTTATCAGGAAAATATGATCACGCTGTGGACCGTGGGCATTTATTAGGCTATGCTTTAGTGGGAGGTCTTAAAGGATT
TGATGCCTCTACAAGCAATAGCGATAACATAGCAACGCAATTATCCTGGGCTAACCAAGCGATGGATAGTGATTCCACTG
GCCAAAATTATTATGAAACAAAAATCAGACGTGCATTGGACAAGAATAAGCGTGTCCGTTACCGTGTTACCCTCATTTAC
GAGGCTGATAATATATTAGCAAGTGGAAGCCATTTAGAAGCAAAATCAGATGACGGCAGTTTAGAATTTAACGTTTTTGT
GCCAAATGTTCAAAAAGGTCTAGAAGTTGACTATCAAACAGGACAAATAACAGTCAGTCACTAA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (95-245)

3D structure

• AlphaFold DB: A0A3L8GIC3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	63.559	82.23	0.523
endA	Streptococcus pneumoniae D39	63.559	82.23	0.523
endA	Streptococcus pneumoniae R6	63.559	82.23	0.523
endA	Streptococcus pneumoniae TIGR4	63.559	82.23	0.523