Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BS1321_RS20675 Genome accession   NZ_CP017704
Coordinates   4283747..4286122 (+) Length   791 a.a.
NCBI ID   WP_063232897.1    Uniprot ID   -
Organism   Peribacillus simplex NBRC 15720 = DSM 1321     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4278747..4291122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS1321_RS20660 (BS1321_20360) spoVB 4279103..4280665 (-) 1563 WP_063232894.1 stage V sporulation protein B -
  BS1321_RS20665 (BS1321_20365) - 4280836..4281141 (+) 306 WP_063232895.1 post-transcriptional regulator -
  BS1321_RS20670 (BS1321_20370) secDF 4281339..4283600 (+) 2262 WP_063232896.1 protein translocase subunit SecDF -
  BS1321_RS20675 (BS1321_20375) recJ 4283747..4286122 (+) 2376 WP_063232897.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BS1321_RS20680 (BS1321_20380) - 4286113..4286625 (+) 513 WP_061464220.1 adenine phosphoribosyltransferase -
  BS1321_RS20685 (BS1321_20385) - 4286942..4289140 (+) 2199 WP_063232898.1 RelA/SpoT family protein -
  BS1321_RS20690 (BS1321_20390) dtd 4289156..4289599 (+) 444 WP_063232899.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 791 a.a.        Molecular weight: 88989.30 Da        Isoelectric Point: 4.7479

>NTDB_id=202066 BS1321_RS20675 WP_063232897.1 4283747..4286122(+) (recJ) [Peribacillus simplex NBRC 15720 = DSM 1321]
MLKPKTRWNLRTADENKVAVLAEELHITPLVAALLVNRGLDTIESARSFLFVKNQTFHDPFLLKDMDKAVYRIREAIQNG
EKIRIFGDYDADGVTSTTVMMTALTRLGADVDFYIPNRFTEGYGPNPMAFRLAAEQGVKVLITVDTGISAVDEAKLAGEL
GMDYILTDHHEPGPELPEALAIIHPKLEDSSYPFKDLAGVGVAFKLAHALLGELPEDLLEIAAIGTIADLVPLQGENRVI
AAKGIEQLRLTRRPGLVALMKVANVQQEALNEESIGFAMAPRINAVGRLGDADPAVDLLMSTNLEEATELANEINDINKE
RQEMVAAMAEEAIAEVEENFPPDSNGVLIIGREGWNAGVVGIVASKLVERFYRPTIVLSFDREKGLAKGSARSIAGFDLF
ESLSTCRELLPHFGGHPMAAGMTLKIEDVQELRDRMNLIAREQLSEEDFTPITNLDGSTTLAEVSIQTIQEMSLLAPFGV
TNPKPKILIDSVQLSSVRKIGANQNHLKVQLEDGENHKLDGVGFGLGHFVDEIAPHAEVSVIGELSINEWNNMKKPQIFV
QDVSVNHWQLFDYRGKGQAEKWLADIPVQNRKIVIFSEDIYGRYPFLQNHPDLVHIQNELDAEQLDCFEGHLVFMDMPPS
REYLKRVIANKNPSRIYAHLSHEQDHFLSTMPTRDHFKWFYAFLAKKGPLDVKRYGDDLAKYRGWSRDTVDFISKVFFEL
DFVTIENGLIMLTTNAKKRDLSESESYTRKKEQFELEQELVYSSYQQLFDWFNHYLVHEATDLEEETKQWI

Nucleotide


Download         Length: 2376 bp        

>NTDB_id=202066 BS1321_RS20675 WP_063232897.1 4283747..4286122(+) (recJ) [Peribacillus simplex NBRC 15720 = DSM 1321]
ATGTTAAAGCCAAAAACCCGGTGGAACTTACGAACTGCCGATGAAAACAAAGTTGCTGTACTAGCTGAAGAGCTTCATAT
CACACCTTTGGTTGCAGCCCTGCTTGTGAATCGAGGCCTGGATACGATTGAAAGTGCCCGATCATTTTTATTTGTAAAAA
ATCAAACTTTTCATGATCCATTTTTATTGAAGGATATGGATAAAGCGGTATATAGAATAAGAGAAGCCATACAAAATGGA
GAAAAAATACGTATATTCGGTGATTATGATGCGGATGGCGTGACATCGACCACAGTGATGATGACGGCGCTGACGAGATT
GGGTGCCGATGTTGACTTTTATATCCCGAATCGGTTTACAGAAGGATATGGTCCGAATCCGATGGCATTCCGCCTTGCAG
CTGAGCAAGGGGTGAAGGTATTAATAACCGTGGATACCGGAATTTCTGCAGTCGATGAAGCGAAACTTGCCGGCGAGCTT
GGCATGGATTATATTTTGACCGACCACCATGAACCGGGACCGGAACTGCCTGAGGCATTGGCAATCATCCATCCTAAACT
TGAGGACAGTTCCTATCCATTTAAAGACCTTGCCGGAGTAGGGGTTGCATTTAAGCTGGCACACGCCCTGCTTGGGGAAT
TGCCTGAGGATTTGCTTGAAATAGCGGCAATCGGCACGATAGCGGATTTAGTGCCTTTACAAGGGGAAAACCGCGTCATT
GCTGCAAAAGGCATCGAGCAGTTACGTTTGACTCGCCGCCCGGGACTTGTTGCCTTAATGAAAGTGGCGAATGTTCAGCA
GGAAGCATTGAATGAAGAATCGATCGGCTTTGCGATGGCACCGCGGATCAATGCTGTCGGGCGCCTTGGAGATGCCGACC
CCGCTGTCGATTTATTGATGTCCACTAACCTGGAAGAAGCAACGGAACTTGCCAATGAAATCAATGATATCAATAAAGAG
CGGCAGGAAATGGTTGCAGCAATGGCGGAAGAAGCGATTGCTGAAGTCGAGGAAAACTTCCCGCCGGATTCCAATGGCGT
GCTCATCATCGGCAGGGAAGGCTGGAACGCCGGAGTTGTAGGGATTGTGGCCTCTAAACTTGTTGAACGCTTTTACCGGC
CCACGATTGTATTGAGCTTTGACCGGGAAAAGGGATTAGCTAAAGGTTCTGCTCGGAGCATAGCCGGTTTCGATCTTTTC
GAAAGTCTTTCCACCTGCCGTGAATTACTGCCGCATTTTGGCGGACATCCAATGGCTGCTGGAATGACATTGAAAATTGA
AGATGTTCAGGAGCTTAGGGACCGCATGAACTTGATTGCGAGGGAACAACTTAGCGAAGAAGACTTCACACCCATTACCA
ATTTGGACGGGTCTACTACATTAGCTGAAGTTTCTATTCAAACGATTCAGGAGATGAGCTTGCTGGCGCCATTTGGGGTG
ACGAATCCCAAACCGAAGATCTTGATAGATTCCGTCCAGCTTTCAAGTGTCCGCAAAATTGGTGCCAACCAAAATCACTT
GAAGGTCCAGCTGGAAGATGGTGAAAATCACAAACTTGACGGTGTGGGCTTCGGTCTCGGTCATTTTGTCGATGAAATAG
CTCCGCACGCTGAAGTCTCGGTTATTGGTGAGCTTTCCATCAATGAGTGGAATAATATGAAAAAGCCTCAGATCTTTGTC
CAAGATGTTTCGGTAAACCATTGGCAGTTATTTGATTACCGCGGTAAGGGACAGGCAGAGAAGTGGCTTGCCGACATTCC
AGTACAAAACCGGAAGATCGTCATTTTTTCGGAAGATATTTATGGCAGATATCCATTTTTGCAAAACCATCCAGATCTCG
TTCATATCCAGAATGAACTGGATGCAGAGCAGCTCGACTGCTTCGAAGGTCATTTGGTATTTATGGACATGCCGCCGTCC
AGGGAATATTTGAAACGGGTGATTGCCAATAAGAACCCTTCGCGTATCTACGCTCACCTTTCCCATGAACAGGACCATTT
CTTAAGTACGATGCCAACAAGGGATCATTTTAAATGGTTTTATGCCTTCCTTGCCAAAAAGGGGCCGCTCGACGTGAAAA
GATATGGTGATGACCTAGCCAAATATCGTGGCTGGTCAAGAGATACCGTGGATTTCATTTCTAAGGTGTTTTTTGAGCTT
GATTTTGTTACAATAGAGAATGGGTTAATTATGCTCACAACCAATGCGAAAAAACGCGATCTCAGTGAATCTGAATCGTA
TACAAGAAAGAAAGAGCAATTTGAGCTTGAACAAGAGCTTGTTTATTCTTCCTATCAGCAATTATTCGATTGGTTCAATC
ATTATTTAGTTCATGAGGCAACAGACCTTGAGGAGGAAACAAAGCAATGGATTTAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(456-562)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(310-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(569-772)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

54.729

100

0.549

Multiple sequence alignment