Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BKN48_RS03025 Genome accession   NZ_CP017676
Coordinates   523237..525597 (-) Length   786 a.a.
NCBI ID   WP_070547597.1    Uniprot ID   -
Organism   Bacillus subtilis strain VV2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 518237..530597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKN48_RS03005 (BKN48_03005) yrvJ 518311..519867 (+) 1557 WP_015714439.1 SH3 domain-containing protein -
  BKN48_RS03010 (BKN48_03010) dtd 519894..520334 (-) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  BKN48_RS03015 (BKN48_03015) relA 520347..522551 (-) 2205 WP_003229747.1 GTP diphosphokinase -
  BKN48_RS03020 (BKN48_03020) aptA 522719..523231 (-) 513 WP_003229745.1 adenine phosphoribosyltransferase -
  BKN48_RS03025 (BKN48_03025) recJ 523237..525597 (-) 2361 WP_070547597.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BKN48_RS03030 (BKN48_03030) yrvD 525664..525987 (-) 324 WP_003229740.1 LapA family protein -
  BKN48_RS03035 (BKN48_03035) yrvC 526063..526560 (-) 498 WP_003229739.1 cation:proton antiporter regulatory subunit -
  BKN48_RS03040 (BKN48_03040) secDF 526718..528931 (-) 2214 WP_014480434.1 protein translocase subunit SecDF -
  BKN48_RS03045 (BKN48_03045) comN 528970..529266 (-) 297 WP_003229735.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88032.70 Da        Isoelectric Point: 5.2328

>NTDB_id=201474 BKN48_RS03025 WP_070547597.1 523237..525597(-) (recJ) [Bacillus subtilis strain VV2]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTNEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCSVEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELQEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSALPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSKDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=201474 BKN48_RS03025 WP_070547597.1 523237..525597(-) (recJ) [Bacillus subtilis strain VV2]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTAATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTGGATGTCATCATTACTGATCATCATGAACCGGGACCGGAGCTTCCAGATGTCCATGCGATCGTCCACCCGAAGCA
ACCTGGCTGCACTTACCCGTTTAAGGAGCTTGCCGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTCTATTGGGTGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACAGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTTGGAAGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGAATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCCGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGCTTCTACCGACCGGCCA
TTGTTCTTGGCATTGATGAAGAAAAGGGAATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCTGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAACGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCAGCGTCGAGGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTTAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTACTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTCAAGAAGGCATCGTACCGGGTT
CAAGAATTTCCATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGCCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACATTACTTCAAACTGAAGACCTTCGCCGAGAAGTGCACGTCATCA
GTTCTAAAGATCAAGCAAAGGCGTTTGACCTCGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCACGTTTACTGGAAGGCAAGGCGCCTGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGAAAAAACACGGTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

99.618

100

0.996

Multiple sequence alignment