Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   BHF65_RS02960 Genome accession   NZ_CP017107
Coordinates   619547..620377 (+) Length   276 a.a.
NCBI ID   WP_069468886.1    Uniprot ID   A0A1D7TQH8
Organism   Ligilactobacillus salivarius strain CICC 23174     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 614547..625377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHF65_RS02945 (BHF65_02945) - 616128..616442 (-) 315 WP_003704272.1 metalloregulator ArsR/SmtB family transcription factor -
  BHF65_RS02950 (BHF65_02950) - 616592..618523 (+) 1932 WP_069468884.1 heavy metal translocating P-type ATPase -
  BHF65_RS02955 (BHF65_02955) - 618577..619179 (-) 603 WP_069468885.1 nitroreductase family protein -
  BHF65_RS02960 (BHF65_02960) endA 619547..620377 (+) 831 WP_069468886.1 DNA/RNA non-specific endonuclease Machinery gene
  BHF65_RS02965 (BHF65_02965) - 620432..620698 (-) 267 WP_003701014.1 hypothetical protein -
  BHF65_RS02970 (BHF65_02970) brnQ 621237..622619 (+) 1383 WP_069468887.1 branched-chain amino acid transport system II carrier protein -
  BHF65_RS02975 (BHF65_02975) dhaS 622766..623290 (+) 525 WP_069468888.1 dihydroxyacetone kinase transcriptional activator DhaS -
  BHF65_RS02980 (BHF65_02980) - 623291..624247 (-) 957 WP_069468889.1 DhaKLM operon coactivator DhaQ -

Sequence

Protein


Download         Length: 276 a.a.        Molecular weight: 30438.78 Da        Isoelectric Point: 10.4970

>NTDB_id=195632 BHF65_RS02960 WP_069468886.1 619547..620377(+) (endA) [Ligilactobacillus salivarius strain CICC 23174]
MARKKKNKSNKFMRWLATVAVGVALAAVGVNTSHISTPFNNEVVNSSTYNKKDYTPTEEQASVVLTDNVRQQLGGNLSFN
GAGAFIVNNNKTNLNTKVSSAPYAVNEVDSLNRPTVANAWLNRTSRQYKNRQATGNGRTSWKPQGFRQVQNLPGRFKHAY
DRGHLLAYALVGNVRGFDASESNEKNIATQTAWANESASKDSTGQNYYEGLVRKALDRNKQVRYRVTDVYDGNNLVPAGA
HLEAKSSDGSLEFNVFVPNVQTNININYATGQATRK

Nucleotide


Download         Length: 831 bp        

>NTDB_id=195632 BHF65_RS02960 WP_069468886.1 619547..620377(+) (endA) [Ligilactobacillus salivarius strain CICC 23174]
ATGGCAAGAAAAAAGAAAAATAAATCAAATAAATTTATGAGATGGTTAGCTACCGTAGCAGTAGGGGTTGCTTTAGCAGC
CGTGGGAGTTAATACAAGTCATATCAGTACACCTTTTAATAATGAAGTTGTGAATAGCAGTACATATAATAAGAAAGACT
ACACACCTACAGAGGAGCAGGCGAGTGTAGTATTAACAGATAATGTACGCCAACAATTAGGTGGTAATTTAAGCTTTAAT
GGGGCAGGAGCTTTCATTGTAAATAATAATAAGACTAACTTAAATACAAAAGTTTCTAGTGCACCATATGCTGTTAATGA
GGTTGATTCATTGAATCGACCAACCGTTGCGAATGCTTGGCTAAATAGAACTAGTCGTCAATATAAAAATCGTCAAGCTA
CAGGAAATGGGCGTACAAGTTGGAAGCCACAAGGATTTAGACAGGTACAAAACTTACCAGGTAGATTCAAGCATGCTTAT
GATAGAGGACATTTATTAGCATATGCGCTTGTGGGAAATGTTAGAGGGTTTGATGCTTCTGAATCTAATGAAAAGAATAT
AGCCACTCAAACTGCATGGGCTAATGAATCAGCTTCTAAGGATTCTACCGGACAAAATTACTACGAAGGATTGGTAAGAA
AAGCTTTAGATAGAAACAAGCAGGTTCGTTATCGTGTGACAGATGTCTATGATGGTAATAATCTAGTGCCAGCAGGAGCA
CATTTGGAAGCTAAATCATCAGATGGTTCGCTAGAATTTAATGTATTTGTGCCTAATGTACAAACTAACATAAATATTAA
TTATGCTACAGGACAAGCTACTAGGAAATAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(83-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D7TQH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

60.538

80.797

0.489
  endA Streptococcus pneumoniae D39

60.538

80.797

0.489
  endA Streptococcus pneumoniae R6

60.538

80.797

0.489
  endA Streptococcus pneumoniae TIGR4

60.538

80.797

0.489

Multiple sequence alignment