Detailed information NTG0194583

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: S100141_RS05755
• Genome accession: NZ_CP021669
• Coordinates: 1066112..1068529 (+)
• Length: 805 a.a.
• NCBI ID: WP_011197970.1
• Uniprot ID: Q65JS7
• Organism: Bacillus licheniformis strain SRCM100141
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1061112..1073529

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S100141_RS05730 (S100141_01173)	-	1062771..1063646 (+)	876	WP_003181655.1	YicC/YloC family endoribonuclease	-
S100141_RS05735 (S100141_01174)	remA	1063722..1063991 (+)	270	WP_003181657.1	extracellular matrix/biofilm regulator RemA	-
S100141_RS05740 (S100141_01175)	gmk	1063999..1064613 (+)	615	WP_003181659.1	guanylate kinase	-
S100141_RS05745 (S100141_01176)	rpoZ	1064616..1064816 (+)	201	WP_003181661.1	DNA-directed RNA polymerase subunit omega	-
S100141_RS05750 (S100141_01177)	coaBC	1064898..1066115 (+)	1218	WP_003181663.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
S100141_RS05755 (S100141_01178)	priA	1066112..1068529 (+)	2418	WP_011197970.1	primosomal protein N'	Machinery gene
S100141_RS05760 (S100141_01179)	def	1068559..1069041 (+)	483	WP_009328532.1	peptide deformylase	-
S100141_RS05765 (S100141_01180)	fmt	1069046..1069996 (+)	951	WP_069500462.1	methionyl-tRNA formyltransferase	-
S100141_RS05770 (S100141_01181)	rsmB	1069986..1071332 (+)	1347	WP_003181671.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
S100141_RS05775 (S100141_01182)	rlmN	1071332..1072417 (+)	1086	WP_003181673.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
S100141_RS05780 (S100141_01183)	-	1072425..1073189 (+)	765	WP_003181675.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91315.68 Da
• Isoelectric Point: 9.0887

>NTDB_id=194583 S100141_RS05755 WP_011197970.1 1066112..1068529(+) (priA) [Bacillus licheniformis strain SRCM100141]
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELAGRSVKDIEDILDLAPVLTEE
LLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIGDKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGN
LEVAYKVKQKTNKKMRRMIAPLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSGKTEVYLQSIEKVLEAGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQ
EEMPRYHAKEAAIQRAKYHRCPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYCGYETGMPSSCPECGSEHIRY
FGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLTSFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIP
DFRAAEKTFQLMTQVSGRAGRHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNIMDHYKRDIEQKQLAISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=194583 S100141_RS05755 WP_011197970.1 1066112..1068529(+) (priA) [Bacillus licheniformis strain SRCM100141]
ATGAACTTCGCTGAAGTGATCGTCGATGTTACAACGAAAAATATCGACAGACCGTTTGATTACCGCATCCCCGACAAATT
CAAAGGTATGATTAAAAAAGGGATGCGCGTGATCGTTCCGTTCGGCGCCCGCAAAATCCAGGGATTTGTCACCAGCGTGA
AAGAATCGTCGGAGCTTGCCGGCCGTTCGGTTAAGGATATCGAGGATATCCTTGATCTGGCGCCCGTTTTGACGGAGGAG
CTGCTTGAGCTGTCAGACTGGCTGACAGAAAAAACACTTTCTTTTAAAATTACGGCTTTGCAGGCGATGCTCCCCGCGGC
TATGAAAGCAAAATACGGCAAAGAAATCAAGGTGATCGGCGATAAACCGCTTCCCGAACCGCTAAAAACCTTGTTCTCGC
AAAAAGAGACGCTTTCTTATTCCGATATAAATGACCACGGAACATTAAAGCTGATTCAGCGCCAGGTTCAAGAAGGCAAT
CTTGAAGTGGCGTATAAAGTAAAGCAGAAAACAAACAAAAAAATGCGGAGAATGATCGCCCCCCTTTGCGCGAAAGAACA
TTTAAAAGAAGCGCTTGCCGGCCTCTCTCCGCAAGCTGTCAAACAAAAAGCGATCCTTCAGTTTTTCATCGACCGCAAAG
AGGGCGGCGCTGTTCCGGCAAAGGATCTGATCGACAAAACGGGGGGCTCTTCCGCGTCCTTGAAAGCGCTCATCGACAAG
GGGCTGTTAAAGGAAAGCGAAGAGGAGGTTTACAGGGATCCTTATCAGGACAGAATGTTCAAAAAAACCGAACCGCTTCC
GCTGACTGAAGAGCAGGCGCAGGCCTACGAGGCGATAGCGGATGCAATCGAAGAAAAGCGGCACGATGTGTTTCTTCTTC
ACGGAGTGACCGGAAGCGGAAAAACGGAAGTCTATCTGCAATCGATCGAAAAGGTGCTGGAAGCCGGCAAAGAAGCGATC
GTTCTCGTTCCGGAAATTTCCCTGACGCCGCAGATGGTCAACCGCTTTAAAGGGCGCTTTGGCTCGCAGGTAGCCGTACT
GCACAGCGGGCTTTCAACAGGAGAAAAATATGATGAATGGCGGAAAATCCACCGCAAAGAAGTCCGGCTTGTCGTCGGGG
CGCGCTCGGCGATTTTCGCGCCGTTTGAAAATCTCGGCATGATTATAATTGATGAAGAGCACGAAACATCGTACAAGCAG
GAGGAGATGCCGAGATACCATGCGAAAGAAGCGGCGATTCAAAGGGCCAAATACCACCGGTGTCCGGTCGTGCTCGGCAG
TGCGACGCCGACGCTCGAATCGTTCGCGAGGGCGAAAAAAGGCGTGTACAAGCTTCTTTCTCTCAAGCACCGCGTCAATC
ACCAAGTCATGCCCGACGTGTCTCTCGTCGATATGAGAGAGGAACTGCGCAGCGGAAACCGCTCGATGTTTTCCAAAGAC
TTAATGGCCGGGCTTGAGGAAACTCTTGAAAAAGGAGAGCAGGCCGTTCTGTTTTTAAACAAAAGAGGCTATTCCTCATT
TGTCATGTGCAGGGACTGCGGATATGTCATCCAGTGTCCTCATTGTGAAATTTCATTGACGTACCACAGGTATGGACAGC
GTCTGAAGTGCCATTACTGCGGCTATGAAACCGGCATGCCGTCAAGCTGCCCCGAATGCGGAAGCGAGCATATCCGCTAC
TTTGGTACAGGCACACAGCGGGTCGAAGAAGAATTGACGAAGGTTCTGCCGGCTGCAAGGGTGATCCGCATGGATGTCGA
TACAACCTCAAGAAAGGGAGCGCATGAAAAGCTGCTCACATCCTTTGGCAGAGGCGAAGCCGATATCCTGCTTGGAACGC
AGATGATCGCCAAAGGGCTCGATTTCGAAAACGTCACGCTCGTCGGTGTCTTGAGCGCGGATACGATGCTTCACATCCCC
GATTTCAGAGCCGCTGAAAAAACGTTCCAGCTGATGACGCAGGTGAGCGGAAGAGCGGGGCGCCACGAAAAGCCGGGCAA
GGTGATCATTCAGACGTACACACCGTCCCATTACAGCATTCAGCTCACAAAAACGCATGATTATGAAACATTCTTCCGGC
AGGAGATGCTGCACAGAAGGGAACAGGCCTATCCTCCATATTATTATTTGGCGCTGGTCAGCGTATCGCACGAGGAAGTG
ACGAAGGCGGCACTCGTGACTGAAAAAATCGCCACCTATTTAAAAAAGAACATTTCAAGCGAGGCGAAGATTCTCGGCCC
TGTCGCATCTCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAAACCAGCTTA
CATCGTTGCTTAAAAACATTATGGATCATTACAAACGAGACATTGAACAAAAGCAATTGGCGATTTCGATCGACATGAAT
CCATATATGATGATGTAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Helicase conserved C-terminal domain (Helicase_C) (578-662)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-543)

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

3D structure

• AlphaFold DB: Q65JS7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	79.006	100	0.79