Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BGI23_RS05765 Genome accession   NZ_CP017016
Coordinates   1109613..1113128 (+) Length   1171 a.a.
NCBI ID   WP_070805469.1    Uniprot ID   -
Organism   Bacillus sp. ABP14     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1104613..1118128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BGI23_RS05730 (BGI23_05730) - 1104747..1105538 (-) 792 WP_070805465.1 phosphotransferase -
  BGI23_RS05735 (BGI23_05735) cspA 1105891..1106094 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  BGI23_RS05740 (BGI23_05740) - 1106431..1106649 (-) 219 WP_070805466.1 hypothetical protein -
  BGI23_RS05745 (BGI23_05745) - 1106958..1107143 (-) 186 WP_070805467.1 hypothetical protein -
  BGI23_RS05750 (BGI23_05750) - 1107428..1108009 (+) 582 WP_070805468.1 competence protein ComK -
  BGI23_RS05755 (BGI23_05755) - 1108283..1108876 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  BGI23_RS05760 (BGI23_05760) lepB 1108933..1109496 (+) 564 WP_000751898.1 signal peptidase I -
  BGI23_RS05765 (BGI23_05765) addB 1109613..1113128 (+) 3516 WP_070805469.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BGI23_RS05770 (BGI23_05770) addA 1113125..1116850 (+) 3726 WP_070805470.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BGI23_RS05775 (BGI23_05775) - 1116863..1117150 (+) 288 WP_070805471.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  BGI23_RS05780 (BGI23_05780) gerPF 1117184..1117399 (-) 216 WP_001141566.1 spore germination protein GerPF -
  BGI23_RS05785 (BGI23_05785) - 1117442..1117828 (-) 387 WP_000902334.1 spore germination protein GerPE -
  BGI23_RS05790 (BGI23_05790) gerPD 1117844..1118038 (-) 195 WP_001052802.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134131.22 Da        Isoelectric Point: 5.2121

>NTDB_id=194478 BGI23_RS05765 WP_070805469.1 1109613..1113128(+) (addB) [Bacillus sp. ABP14]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTIHTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=194478 BGI23_RS05765 WP_070805469.1 1109613..1113128(+) (addB) [Bacillus sp. ABP14]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTATTCCAAAAAGCAGCGGAGCAAAATGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAAAGATATA
ACGTGACACCATCAAACGTATATGAAATGTGGCAACAATTAGATGCACATAGTAGCAGTGCTGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTACTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGCTTCCATTCGT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGAGAGTGACAATTACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCGCTTATGGAACAACCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAGAAATTTAACGGGGAAGCAAGTGTAACGATTCATACAGCCGCGAATTTA
CGAGCTGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
TCTTCTTCGGAACGGAGAAAGTTATTACGATGTAATGCGGACTCTATTTACAGATTATAATATCCCGCACTTTATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAAATGGA
TGAGTTTGAAAACTACTGTTTAGCATACGGTGTACAAGGGAAAAGATGGACTTCTGAAGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACGGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTCGTCATTGGAGTGAATGAAGGTG
TGATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTTGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCATGCCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAACGACGTAAATGATTTATCACGTTCGGAACAAATTTCATATGTAGCAACACCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGCAACTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTTGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATCGGAGAGCTCTTCCATGCAGCGCTGAAGAAAATTGCGGACAGGCTATTACGTGAAAATCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAGCA
CGCGAAATCGAGTGGTTTCGTACCAGTTGATTTAGAAGTACCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAATGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGTACAGCATCACCAGCGGGTG
TATTGTATTTCCACATTCATAACCCAATTGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTGATCGATATTGCCCCT
TACAAAAAAGGAAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCGGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(302-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493

Multiple sequence alignment