Detailed information NTG0193500

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: S101267_RS08790
• Genome accession: NZ_CP021505
• Coordinates: 1646412..1648823 (+)
• Length: 803 a.a.
• NCBI ID: WP_013352214.1
• Uniprot ID: A0A9P1JGW2
• Organism: Bacillus amyloliquefaciens strain SRCM101267
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1641412..1653823

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S101267_RS08765 (S101267_01756)	-	1643054..1643929 (+)	876	WP_013352211.1	YicC/YloC family endoribonuclease	-
S101267_RS08770 (S101267_01757)	remA	1644006..1644275 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
S101267_RS08775 (S101267_01758)	gmk	1644283..1644897 (+)	615	WP_013352212.1	guanylate kinase	-
S101267_RS08780 (S101267_01759)	rpoZ	1644901..1645104 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
S101267_RS08785 (S101267_01760)	coaBC	1645195..1646415 (+)	1221	WP_013352213.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
S101267_RS08790 (S101267_01761)	priA	1646412..1648823 (+)	2412	WP_013352214.1	primosomal protein N'	Machinery gene
S101267_RS08795 (S101267_01762)	def	1648847..1649329 (+)	483	WP_013352215.1	peptide deformylase	-
S101267_RS08800 (S101267_01763)	fmt	1649334..1650287 (+)	954	WP_013352216.1	methionyl-tRNA formyltransferase	-
S101267_RS08805 (S101267_01764)	rsmB	1650274..1651617 (+)	1344	WP_065118558.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
S101267_RS08810 (S101267_01765)	rlmN	1651621..1652712 (+)	1092	WP_013352218.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
S101267_RS08815 (S101267_01766)	-	1652718..1653479 (+)	762	WP_013352219.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90972.05 Da
• Isoelectric Point: 8.8944

>NTDB_id=193500 S101267_RS08790 WP_013352214.1 1646412..1648823(+) (priA) [Bacillus amyloliquefaciens strain SRCM101267]
MNVAEVIVDVSSKNIDRPFDYRIPDHLRGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGQMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIRQLFRNQKSLLYSDITDERILSALQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIDGGAKISAAELCQKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKKAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVKLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSKELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=193500 S101267_RS08790 WP_013352214.1 1646412..1648823(+) (priA) [Bacillus amyloliquefaciens strain SRCM101267]
ATGAATGTGGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGGCCCTTTGATTACAGAATTCCCGATCATTT
AAGAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTCGGCCCGCGCAAGCTTCAAGGGTTCGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGGCAAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACACCCGTTTTGACGGAAGAG
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCGGCCGC
GCTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAGGCAGCTTTTCAGAA
ATCAGAAGAGCCTTCTGTACTCAGATATTACCGATGAACGCATTCTTTCGGCACTGCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACCTATAAAGTCGCCCAGAAAACGAATAAAAAAATGATCCGCATGATTGAGGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAACAGGCTTCTAAACAGCTTGCCGTGCTTACTTATTTTATTGACGGTGGAG
CAAAGATTTCCGCGGCTGAGCTTTGCCAAAAGACCGATTCCAGTTCAGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAACCTCTCTCTCTTAC
TGAAGAGCAACAGGCCGCTTTTGAGCCGATTAAGAAGGCCGTAGCCGATCATGTTCATCACGTCTTTCTGCTTCACGGTG
TGACAGGGAGCGGCAAAACGGAAATTTACCTGCAATCTATTGAAAAGGTGCTGGCTAAAGGAAAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCATTGACGCCGCAAATGGTCAATCGTTTTAAAGGCCGCTTCGGTTCACAGGTTGCGGTCATGCACAG
CGGATTGTCCACAGGTGAAAAATACGATGAATGGCGGAAAATTCAGCGTAAGGAAGTAAAGCTTGTCGTCGGAGCCAGGT
CGGCCATCTTTGCCCCATTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCCTCCTATAAGCAGGAGGAA
ATGCCGCGGTACCATGCAAAAGACGTAGCCATACGCAGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTCGCACGGGCGAAAAAAGGCGTTTATGAGCTGCTCCCGCTGAAACACCGCGTAAATCGCCAGC
TCATGCCGGATGTATCTCTCGTTGATATGAGAGAAGAACTGCGAAACGGAAACAGATCGATGTTTTCAAAAGAGCTGATG
GAACAGCTGGAGGAGAAGCTTGCGAAAAAAGAGCAGGCCGTTCTCTTTCTGAATAAACGGGGATATTCTTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCCCAATGTCCGCATTGCGACATTTCAATGACCTATCACCGCTACGGACAAAGGCTCA
AATGCCATTACTGCGGCCATGAGGAGCCTGTTCCGGGAACGTGCCCGGAATGCGGCAGTGAGCATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTAGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACGAC
CTCAAGAAAAGGCGCTCATGAAAAGCTATTGTCAGCTTTCGGTGAAGGAAAAGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCGGCGGAGAAGACATTTCAGCTGTTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTAGT
CATACAAACGTACACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCATGACTATGAAACTTTCTTTCAGCAGGAGA
TGGCGCACAGGCGGGCCCAGTCATATCCGCCTTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCCGCAAAA
GCCGCGGTCACCGCTGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGACCGGACACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAAATTCTGGAGCATTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

3D structure

• AlphaFold DB: A0A9P1JGW2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	83.851	100	0.841