Detailed information NTG0193354

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: S100757_RS08865
• Genome accession: NZ_CP021499
• Coordinates: 1679380..1681797 (+)
• Length: 805 a.a.
• NCBI ID: WP_069837502.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain SRCM100757
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1674380..1686797

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S100757_RS08840 (S100757_01751)	endC	1676030..1676905 (+)	876	WP_048654878.1	YicC/YloC family endoribonuclease	-
S100757_RS08845 (S100757_01752)	remA	1676982..1677251 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
S100757_RS08850 (S100757_01753)	gmk	1677259..1677873 (+)	615	WP_003232083.1	guanylate kinase	-
S100757_RS08855 (S100757_01754)	rpoZ	1677877..1678080 (+)	204	WP_003221520.1	DNA-directed RNA polymerase subunit omega	-
S100757_RS08860 (S100757_01755)	coaBC	1678163..1679383 (+)	1221	WP_015252114.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
S100757_RS08865 (S100757_01756)	priA	1679380..1681797 (+)	2418	WP_069837502.1	primosomal protein N'	Machinery gene
S100757_RS08870 (S100757_01757)	def	1681824..1682306 (+)	483	WP_014479759.1	peptide deformylase	-
S100757_RS08875 (S100757_01758)	fmt	1682311..1683264 (+)	954	WP_086344597.1	methionyl-tRNA formyltransferase	-
S100757_RS08880 (S100757_01759)	rsmB	1683251..1684594 (+)	1344	WP_087614550.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
S100757_RS08885 (S100757_01760)	rlmN	1684598..1685689 (+)	1092	WP_014476780.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
S100757_RS08890 (S100757_01761)	prpC	1685696..1686460 (+)	765	WP_014479762.1	protein-serine/threonine phosphatase PrpC	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91340.34 Da
• Isoelectric Point: 8.4336

>NTDB_id=193354 S100757_RS08865 WP_069837502.1 1679380..1681797(+) (priA) [Bacillus subtilis subsp. subtilis strain SRCM100757]
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMDLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHDTLKLIQRHVQKGD
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAQLCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=193354 S100757_RS08865 WP_069837502.1 1679380..1681797(+) (priA) [Bacillus subtilis subsp. subtilis strain SRCM100757]
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
CAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCATTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGGAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGGATCTGTCATCATGGCTGTCAGACAAAACGCTGTCTTTTAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGCACACGGCGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGATACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGAC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGGCTGAGGGATTGTCCCGCCAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCGGCGCAATTGTGCAAAAAAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCAGGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTCGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACGGAAATTTATCTGCAATCAATTGAAAAAGTACTGGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCCGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCCCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCCGCCATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAACACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAATCATATGCAAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ATCGGGTGATGCCCGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGCTCGATGTTTTCGGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCAAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTATTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTCGGAACGGGAACACAGCGAGTGGAGGAAGAGCTGACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATCGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAAACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCGCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAAATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
AAGCGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CTGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

• Helicase conserved C-terminal domain (Helicase_C) (565-662)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-545)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	99.627	100	0.996