Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AWQ21_RS02775 Genome accession   NZ_CP016474
Coordinates   593634..594734 (-) Length   366 a.a.
NCBI ID   WP_065713223.1    Uniprot ID   -
Organism   Picosynechococcus sp. PCC 7003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 588634..599734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWQ21_RS02755 (AWQ21_02755) - 588889..589473 (-) 585 WP_065713219.1 transposase -
  AWQ21_RS02760 (AWQ21_02760) - 589541..591811 (-) 2271 WP_065713220.1 AMIN domain-containing protein -
  AWQ21_RS02765 (AWQ21_02765) - 592048..592845 (-) 798 WP_065713221.1 hypothetical protein -
  AWQ21_RS02770 (AWQ21_02770) - 592898..593629 (-) 732 WP_065713222.1 PilN domain-containing protein -
  AWQ21_RS02775 (AWQ21_02775) pilM 593634..594734 (-) 1101 WP_065713223.1 type IV pilus assembly protein PilM Machinery gene
  AWQ21_RS16460 - 594959..595465 (+) 507 WP_232315042.1 helix-turn-helix domain-containing protein -
  AWQ21_RS16465 - 595449..596207 (+) 759 WP_232315113.1 transposase -
  AWQ21_RS02785 (AWQ21_02785) - 596459..597739 (+) 1281 WP_065713224.1 ABC transporter substrate-binding protein -
  AWQ21_RS02790 (AWQ21_02790) - 597944..598387 (+) 444 WP_012306130.1 DUF3531 family protein -
  AWQ21_RS02795 (AWQ21_02795) - 598380..599051 (+) 672 WP_065713225.1 RibD family protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40173.22 Da        Isoelectric Point: 4.2510

>NTDB_id=188500 AWQ21_RS02775 WP_065713223.1 593634..594734(-) (pilM) [Picosynechococcus sp. PCC 7003]
MGTAAPKFKPKPKGIGIEISTERLNIVQLKKQGQVYKLATYITEPVPEGIFEEGKIVDVPALAELIQTTLSANNISAKQV
TAAVPMREAIIRLIPIPAELDDAELRDMVLNHEASLYLPYPREEVDLDYQKLGFFQDEDGIDKVQVLLVATRREVTDAYV
ETIVQAGLEINILEINSFALLRTIREQLRQFSSQEAVVLVDIEFDCTEIAIVVDGVPQFSRTVPIGASQLQNALSRAMNL
PAPRDIELLQGMTIPNTPMEGARTGATGINPGMAALLRVLGELTDELRRSIDFYLNQSGELEVAQLMLAGPGGGISQLDE
FFTQRLSIPAIQIDPFEALSIDIDEEISKVQRPSLGTVLGLGLREV

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=188500 AWQ21_RS02775 WP_065713223.1 593634..594734(-) (pilM) [Picosynechococcus sp. PCC 7003]
ATAGGGACTGCCGCGCCCAAGTTCAAACCAAAGCCTAAAGGGATTGGCATCGAAATCTCAACGGAACGCTTAAACATTGT
CCAACTCAAAAAACAGGGGCAGGTCTATAAGCTGGCGACCTATATCACCGAACCCGTGCCCGAAGGCATTTTTGAAGAAG
GGAAAATTGTCGATGTTCCGGCCCTTGCCGAGCTCATCCAAACGACCCTCAGCGCCAACAACATCAGCGCCAAACAGGTC
ACCGCCGCCGTGCCCATGCGGGAAGCGATTATTCGCTTGATCCCGATCCCCGCCGAGCTCGATGATGCCGAACTGCGGGA
TATGGTGCTCAACCACGAAGCGAGCCTCTACCTGCCCTACCCCCGCGAAGAAGTAGATCTTGACTACCAAAAACTTGGAT
TTTTCCAAGATGAAGATGGCATTGATAAGGTGCAAGTCCTCTTGGTGGCCACCCGGCGGGAAGTGACCGACGCCTATGTA
GAAACCATCGTGCAGGCGGGACTAGAGATCAATATCCTCGAAATTAATAGTTTTGCCCTCCTCCGGACGATTCGTGAGCA
ACTGCGGCAGTTTAGCTCCCAGGAAGCGGTGGTCTTGGTCGATATTGAATTTGACTGTACAGAAATTGCCATTGTGGTCG
ATGGGGTGCCGCAATTTTCCCGGACAGTGCCCATTGGCGCTTCGCAACTGCAAAATGCCCTATCCCGCGCCATGAATTTA
CCGGCTCCCCGTGACATCGAACTCCTCCAGGGGATGACCATTCCCAACACACCCATGGAAGGAGCCCGCACTGGCGCAAC
GGGTATTAATCCTGGGATGGCGGCCCTCCTACGCGTTTTAGGGGAACTGACGGACGAATTGCGTCGTTCCATTGATTTTT
ATCTCAACCAAAGCGGTGAATTGGAGGTGGCCCAATTGATGTTGGCTGGCCCCGGCGGTGGGATTAGTCAACTGGATGAA
TTTTTTACGCAGCGTCTCAGCATTCCGGCGATTCAAATTGATCCGTTTGAGGCGCTATCCATTGATATTGATGAAGAGAT
TTCCAAGGTGCAACGACCGTCCTTGGGTACGGTACTGGGTTTAGGACTACGGGAGGTATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(282-364)

Type IV pilus assembly protein PilM; (PilM_2)

(16-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

72.346

97.814

0.708

Multiple sequence alignment