Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A4W81_RS03175 Genome accession   NZ_CP016465
Coordinates   631240..631752 (+) Length   170 a.a.
NCBI ID   WP_041820873.1    Uniprot ID   A0AAX0VCR7
Organism   Latilactobacillus sakei strain TMW 1.3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 626240..636752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W81_RS03135 (A4W81_03115) - 626455..626829 (-) 375 WP_011375004.1 hypothetical protein -
  A4W81_RS03140 (A4W81_03120) - 626926..627425 (+) 500 Protein_595 VanZ family protein -
  A4W81_RS03145 (A4W81_03125) - 627516..628247 (+) 732 WP_076632059.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W81_RS03150 (A4W81_03130) comGA 628363..629253 (+) 891 WP_076632060.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W81_RS03155 (A4W81_03135) comGB 629246..630253 (+) 1008 WP_076632061.1 type II secretion system F family protein Machinery gene
  A4W81_RS03160 (A4W81_03140) comGC 630250..630549 (+) 300 WP_076632062.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W81_RS03165 (A4W81_03145) comGD 630521..630973 (+) 453 WP_076632063.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W81_RS03170 (A4W81_03150) comGE 630960..631265 (+) 306 WP_025016385.1 hypothetical protein Machinery gene
  A4W81_RS03175 (A4W81_03155) comGF 631240..631752 (+) 513 WP_041820873.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W81_RS03180 (A4W81_03160) - 631715..632053 (+) 339 WP_011374995.1 hypothetical protein -
  A4W81_RS03185 (A4W81_03165) - 632177..633187 (+) 1011 WP_011374994.1 class I SAM-dependent methyltransferase -
  A4W81_RS03190 (A4W81_03170) - 633209..634405 (+) 1197 WP_011374993.1 acetate kinase -
  A4W81_RS03195 (A4W81_03175) - 634460..634918 (+) 459 WP_011374992.1 hypothetical protein -
  A4W81_RS03205 (A4W81_03185) - 635461..635826 (-) 366 WP_076632064.1 DUF805 domain-containing protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19734.66 Da        Isoelectric Point: 6.9902

>NTDB_id=188410 A4W81_RS03175 WP_041820873.1 631240..631752(+) (comGF) [Latilactobacillus sakei strain TMW 1.3]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGKLKQLQLDYQRPFVQIDTTFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=188410 A4W81_RS03175 WP_041820873.1 631240..631752(+) (comGF) [Latilactobacillus sakei strain TMW 1.3]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCAGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGTCAATTAGATGGGTATATGCCATTATTAGGCAAACTCAAGCAACTTCAACTTGACTATCAGC
GACCGTTTGTTCAAATCGATACGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATCAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

100

100

1

Multiple sequence alignment