Detailed information NTG0188277

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: BAY21_RS08870
• Genome accession: NZ_CP016439
• Coordinates: 1685820..1686701 (+)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus strain CS8
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1680820..1691701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAY21_RS08845 (BAY21_08845)	-	1682320..1682775 (+)	456	WP_041827047.1	DUF4832 domain-containing protein	-
BAY21_RS08850 (BAY21_08850)	galE	1682790..1683803 (+)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
BAY21_RS08855 (BAY21_08855)	-	1683991..1684227 (+)	237	WP_002946959.1	DUF1146 family protein	-
BAY21_RS08860 (BAY21_08860)	murA	1684297..1685568 (+)	1272	WP_041827046.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
BAY21_RS08865 (BAY21_08865)	-	1685570..1685758 (+)	189	WP_011227277.1	DNA-directed RNA polymerase subunit beta	-
BAY21_RS08870 (BAY21_08870)	endA	1685820..1686701 (+)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
BAY21_RS08875 (BAY21_08875)	-	1686772..1687422 (-)	651	WP_011226088.1	amino acid ABC transporter permease	-
BAY21_RS08880 (BAY21_08880)	-	1687434..1688132 (-)	699	WP_011226087.1	amino acid ABC transporter permease	-
BAY21_RS08885 (BAY21_08885)	-	1688150..1688983 (-)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
BAY21_RS08890 (BAY21_08890)	-	1688995..1689762 (-)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
BAY21_RS08895 (BAY21_08895)	vicR	1690294..1691001 (+)	708	WP_011227274.1	response regulator YycF	Regulator

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=188277 BAY21_RS08870 WP_011226089.1 1685820..1686701(+) (endA) [Streptococcus thermophilus strain CS8]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=188277 BAY21_RS08870 WP_011226089.1 1685820..1686701(+) (endA) [Streptococcus thermophilus strain CS8]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594