Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   A9P12_RS00675 Genome accession   NZ_CP020514
Coordinates   119988..120656 (+) Length   222 a.a.
NCBI ID   WP_001336353.1    Uniprot ID   -
Organism   Escherichia coli strain 219     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 114988..125656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9P12_RS00640 (A9P12_00650) yicC 115008..115871 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  A9P12_RS00650 (A9P12_00660) rph 115998..116714 (+) 717 WP_001247093.1 ribonuclease PH -
  A9P12_RS00655 (A9P12_00665) pyrE 116779..117420 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  A9P12_RS00660 (A9P12_00670) slmA 117457..118053 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  A9P12_RS00665 (A9P12_00675) dut 118160..118618 (-) 459 WP_000976070.1 dUTP diphosphatase -
  A9P12_RS00670 (A9P12_00680) coaBC 118596..119816 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  A9P12_RS00675 (A9P12_00685) radC 119988..120656 (+) 669 WP_001336353.1 DNA repair protein RadC Machinery gene
  A9P12_RS00680 (A9P12_00690) rpmB 120873..121109 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  A9P12_RS00685 (A9P12_00695) rpmG 121130..121297 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  A9P12_RS00690 (A9P12_00700) mutM 121395..122204 (+) 810 WP_001114529.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  A9P12_RS00695 (A9P12_00705) coaD 122243..122722 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  A9P12_RS00700 (A9P12_00710) waaA 122730..124007 (-) 1278 WP_000891566.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  A9P12_RS00705 (A9P12_00715) rfaQ 124414..125484 (+) 1071 WP_001311221.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25290.41 Da        Isoelectric Point: 7.8777

>NTDB_id=187147 A9P12_RS00675 WP_001336353.1 119988..120656(+) (radC) [Escherichia coli strain 219]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHCRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=187147 A9P12_RS00675 WP_001336353.1 119988..120656(+) (radC) [Escherichia coli strain 219]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATTGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGTTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment