Detailed information NTG0186698

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AOT18_RS14560
• Genome accession: NZ_CP016300
• Coordinates: 3010750..3011868 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain CMC-CR-MDR-Ab66
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3005750..3016868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOT18_RS14550 (AOT18_14305)	-	3008866..3009558 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
AOT18_RS14555 (AOT18_14310)	pilT	3009685..3010722 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AOT18_RS14560 (AOT18_14315)	pilU	3010750..3011868 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AOT18_RS14565 (AOT18_14320)	fur	3011938..3012375 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AOT18_RS14570 (AOT18_14325)	-	3012487..3012885 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AOT18_RS14575 (AOT18_14330)	-	3012940..3013263 (-)	324	WP_000442587.1	RnfH family protein	-
AOT18_RS14580 (AOT18_14335)	-	3013263..3014330 (-)	1068	WP_002019572.1	hypothetical protein	-
AOT18_RS14590 (AOT18_14345)	-	3014483..3014938 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AOT18_RS14595 (AOT18_14350)	-	3015191..3016039 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=186698 AOT18_RS14560 WP_000347039.1 3010750..3011868(+) (pilU) [Acinetobacter baumannii strain CMC-CR-MDR-Ab66]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=186698 AOT18_RS14560 WP_000347039.1 3010750..3011868(+) (pilU) [Acinetobacter baumannii strain CMC-CR-MDR-Ab66]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395