Detailed information NTG0185342

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BSK2_RS08440
• Genome accession: NZ_CP020367
• Coordinates: 1621482..1623899 (+)
• Length: 805 a.a.
• NCBI ID: WP_080529308.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain GQJK2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1616482..1628899

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSK2_RS08415 (BSK2_08415)	endC	1618132..1619007 (+)	876	WP_009967215.1	YicC/YloC family endoribonuclease	-
BSK2_RS08420 (BSK2_08420)	remA	1619084..1619353 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
BSK2_RS08425 (BSK2_08425)	gmk	1619361..1619975 (+)	615	WP_003232083.1	guanylate kinase	-
BSK2_RS08430 (BSK2_08430)	rpoZ	1619979..1620182 (+)	204	WP_003221520.1	DNA-directed RNA polymerase subunit omega	-
BSK2_RS08435 (BSK2_08435)	coaBC	1620265..1621485 (+)	1221	WP_015252114.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BSK2_RS08440 (BSK2_08440)	priA	1621482..1623899 (+)	2418	WP_080529308.1	primosomal protein N'	Machinery gene
BSK2_RS08445 (BSK2_08445)	def	1623926..1624408 (+)	483	WP_029726494.1	peptide deformylase	-
BSK2_RS08450 (BSK2_08450)	fmt	1624413..1625366 (+)	954	WP_080529309.1	methionyl-tRNA formyltransferase	-
BSK2_RS08455 (BSK2_08455)	rsmB	1625353..1626696 (+)	1344	WP_021479311.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
BSK2_RS08460 (BSK2_08460)	rlmN	1626700..1627791 (+)	1092	WP_021479310.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
BSK2_RS08465 (BSK2_08465)	prpC	1627798..1628562 (+)	765	WP_014479762.1	protein-serine/threonine phosphatase PrpC	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91355.35 Da
• Isoelectric Point: 8.3333

>NTDB_id=185342 BSK2_RS08440 WP_080529308.1 1621482..1623899(+) (priA) [Bacillus subtilis strain GQJK2]
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMDLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGD
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=185342 BSK2_RS08440 WP_080529308.1 1621482..1623899(+) (priA) [Bacillus subtilis strain GQJK2]
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACTAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
GAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCGTTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGAAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGGATCTGTCATCATGGCTGTCAGACAAAACGCTGTCTTTTAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGCACACGGCGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGAGACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGAC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGGCTGAGGGATTGTCCCGCCAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCGGCGGAATTGTGCAAAAAAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCAGGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTCGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACCGAAATTTATCTGCAATCAATTGAAAAAGTACTGGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCGGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCCCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCCGCCATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAGCACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAATCATATGCTAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ATCGGGTGATGCCCGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGCTCGATGTTTTCAGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCCAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTATTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTCGGAACGGGAACACAGCGAGTGGAGGAAGAGCTGACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATCGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAAACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCGCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAGATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
TAGCGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CCGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

• Helicase conserved C-terminal domain (Helicase_C) (565-662)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-545)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	99.876	100	0.999