Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   DA68_RS18080 Genome accession   NZ_CP015727
Coordinates   3647400..3649739 (-) Length   779 a.a.
NCBI ID   WP_025966352.1    Uniprot ID   -
Organism   Bacillus cereus strain A1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3642400..3654739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA68_RS30840 (DA68_18060) - 3643647..3643823 (-) 177 WP_001242605.1 hypothetical protein -
  DA68_RS18065 (DA68_18065) - 3643989..3644429 (-) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  DA68_RS18070 (DA68_18070) relA 3644446..3646629 (-) 2184 WP_001262811.1 GTP diphosphokinase -
  DA68_RS18075 (DA68_18075) - 3646840..3647352 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  DA68_RS18080 (DA68_18080) recJ 3647400..3649739 (-) 2340 WP_025966352.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  DA68_RS18085 (DA68_18085) - 3649841..3650734 (-) 894 WP_000409741.1 cation diffusion facilitator family transporter -
  DA68_RS18090 (DA68_18090) secDF 3650891..3653155 (-) 2265 WP_001119061.1 protein translocase subunit SecDF -
  DA68_RS18095 (DA68_18095) - 3653425..3653709 (-) 285 WP_000886978.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88074.66 Da        Isoelectric Point: 4.9694

>NTDB_id=181838 DA68_RS18080 WP_025966352.1 3647400..3649739(-) (recJ) [Bacillus cereus strain A1]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENKFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGI
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
GGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=181838 DA68_RS18080 WP_025966352.1 3647400..3649739(-) (recJ) [Bacillus cereus strain A1]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCATTTCGTTGGGCGC
ATAGTGCAGGTTTCTCACTGATTATTACTGTCGACACTGGCATCGCAGCAGTACATGAAGCGAATGTAGCGAAAGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCGCTTGCGATTATACATCCGAAATT
AGAAGGTGGCGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAGAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCTCGTATTAATGCGGTTGGGCGTTTAGAAGATGCTGCGC
CGGCTGTACACCTTTTATTATCAGATGATCCAGAGGAAGCGAAAGAGTTGGCCGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTTGAAAATAAATTCCCGCCAGAAGAAAATAAAGT
ATTAGTACTTGCAAAAGAAGGCTGGAACCCAGGGGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATCGATCCTATAAAAGAAACAGCAAAAGGCTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAACTTATCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGATTAAATGAACAAGCAGATGCGATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGCAAAGTAGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAAAAACTAGCCCCGTTTGGTATT
GGAAATCCGAAACCACGTATTGCAGTAAAAGATGCAGAGTTAGAAAGCATTCGCGCAATCGGATCAGATGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCATCCATCAATGAATGGAATAATTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACCGATGAATTACGTGAG
TTATTTAAGGCAGGATTCCCAGCGCGAATTTATACGCTATTTTATCAAGAGAATAACCACTTATTTAGTACAGTTCCGAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTTATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GGTGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHHA1 domain (DHHA1)

(315-439)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.455

99.358

0.521

Multiple sequence alignment