Detailed information NTG0181588

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A7319_RS03885
• Genome accession: NZ_CP015638
• Coordinates: 883877..884743 (-)
• Length: 288 a.a.
• NCBI ID: WP_069075202.1
• Uniprot ID: A0AB36Y547
• Organism: Pseudomonas fluorescens strain L111
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 878877..889743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A7319_RS03845 (A7319_03840)	-	879266..880564 (-)	1299	WP_017845476.1	NAD(P)/FAD-dependent oxidoreductase	-
A7319_RS03850 (A7319_03845)	-	880625..880798 (-)	174	WP_024073379.1	DUF3094 family protein	-
A7319_RS03855 (A7319_03850)	-	880888..881517 (-)	630	WP_017845475.1	DUF1780 domain-containing protein	-
A7319_RS03860 (A7319_03855)	-	881618..882085 (+)	468	WP_069075203.1	FAD/FMN-containing dehydrogenase	-
A7319_RS03865 (A7319_03860)	-	882082..882771 (+)	690	WP_024073381.1	energy-coupling factor ABC transporter permease	-
A7319_RS03870 (A7319_03865)	-	882848..883063 (+)	216	WP_003171680.1	hypothetical protein	-
A7319_RS03875 (A7319_03870)	yacG	883060..883260 (-)	201	WP_017845472.1	DNA gyrase inhibitor YacG	-
A7319_RS03880 (A7319_03875)	coaE	883257..883880 (-)	624	WP_024073382.1	dephospho-CoA kinase	-
A7319_RS03885 (A7319_03880)	pilD	883877..884743 (-)	867	WP_069075202.1	prepilin peptidase	Machinery gene
A7319_RS03890 (A7319_03885)	pilC	884740..885948 (-)	1209	WP_069075201.1	type II secretion system F family protein	Machinery gene
A7319_RS03895 (A7319_03890)	-	886150..886563 (+)	414	WP_024073385.1	pilin	-
A7319_RS03900 (A7319_03895)	-	886598..888007 (+)	1410	WP_069075200.1	O-antigen ligase family protein	-
A7319_RS03910 (A7319_03905)	-	888270..888575 (+)	306	WP_024073387.1	DUF6388 family protein	-
A7319_RS03915 (A7319_03910)	nadC	888697..889545 (-)	849	WP_024073388.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31860.16 Da
• Isoelectric Point: 7.0716

>NTDB_id=181588 A7319_RS03885 WP_069075202.1 883877..884743(-) (pilD) [Pseudomonas fluorescens strain L111]
MNLLLTEQPWAFVAVALVLGLIIGSFLNVLVWRLPKMLEREWRAQALEVLGMPADPAGPTYNLMHPNSCCPHCNHPIRPW
ENIPLLSYLMLRGRCAHCQQPISARYPITELACGLISAMVAWHFGFGWQAGAVMLLSWGLLAMSLIDVDHQLLPDILVLP
LLWLGLIINSADLLTALPDALWGAVIGYLSLWTVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMSSLVGVI
VGLILMRLRKAEISAPMPFGPYLAIAGWIAVLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=181588 A7319_RS03885 WP_069075202.1 883877..884743(-) (pilD) [Pseudomonas fluorescens strain L111]
TTGAACCTGCTGCTGACTGAGCAGCCATGGGCGTTTGTCGCAGTGGCCCTGGTACTGGGGCTGATCATCGGTAGCTTCCT
CAATGTACTGGTATGGCGCCTGCCGAAAATGCTTGAGCGGGAATGGCGCGCACAAGCCCTTGAAGTGCTCGGCATGCCCG
CCGACCCCGCCGGGCCCACCTACAACCTGATGCACCCCAACTCCTGCTGCCCGCACTGCAATCACCCGATTCGCCCGTGG
GAGAATATCCCGCTGCTCAGCTACCTCATGCTCCGGGGGCGTTGCGCCCATTGTCAGCAGCCGATCAGTGCACGCTACCC
CATCACTGAACTGGCCTGCGGGCTGATCTCGGCCATGGTGGCCTGGCATTTTGGCTTTGGCTGGCAAGCCGGGGCAGTCA
TGTTGTTGAGCTGGGGGCTGCTGGCAATGAGCCTGATCGATGTGGATCACCAACTGCTACCCGATATCCTGGTCCTGCCG
CTGCTGTGGCTGGGGCTGATCATCAACAGCGCCGACCTGCTGACCGCACTGCCGGACGCACTGTGGGGCGCGGTGATCGG
CTACCTAAGTCTGTGGACAGTGTTCTGGCTGTTCAAACTGGTGACCGGCAAGGACGGCATGGGCCATGGTGACTTCAAGT
TATTGGCGCTGCTTGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACGCTACTGATGTCTTCGCTGGTGGGCGTGATC
GTAGGGCTGATTCTGATGCGCTTGCGCAAGGCTGAGATCTCGGCACCGATGCCGTTTGGTCCCTATCTGGCAATTGCCGG
CTGGATTGCCGTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (134-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.317	96.528	0.524
pilD	Vibrio campbellii strain DS40M4	51.254	96.875	0.497
pilD	Acinetobacter nosocomialis M2	51.282	94.792	0.486
pilD	Acinetobacter baumannii D1279779	51.282	94.792	0.486
pilD	Neisseria gonorrhoeae MS11	49.259	93.75	0.462