Detailed information NTG0181566

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BZK09_RS06445
• Genome accession: NZ_CP019595
• Coordinates: 1254818..1257226 (+)
• Length: 802 a.a.
• NCBI ID: WP_000560853.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain GD1677
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1249818..1262226

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BZK09_RS06420 (BZK09_06430)	-	1249926..1250327 (+)	402	WP_000354182.1	VOC family protein	-
BZK09_RS06425 (BZK09_06435)	-	1250590..1252287 (-)	1698	WP_000312759.1	NFACT family protein	-
BZK09_RS06430 (BZK09_06440)	gmk	1252562..1253185 (+)	624	WP_000368225.1	guanylate kinase	-
BZK09_RS06435 (BZK09_06445)	rpoZ	1253185..1253403 (+)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
BZK09_RS06440 (BZK09_06450)	coaBC	1253619..1254818 (+)	1200	WP_000722168.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BZK09_RS06445 (BZK09_06455)	priA	1254818..1257226 (+)	2409	WP_000560853.1	primosomal protein N'	Machinery gene
BZK09_RS06450 (BZK09_06460)	-	1257729..1258700 (+)	972	WP_000751710.1	hypothetical protein	-
BZK09_RS06455 (BZK09_06465)	-	1258838..1259041 (-)	204	WP_001198022.1	TM2 domain-containing protein	-
BZK09_RS06460 (BZK09_06470)	-	1259340..1259828 (+)	489	WP_000985287.1	peptide deformylase	-
BZK09_RS06465 (BZK09_06475)	fmt	1259821..1260756 (+)	936	WP_000161301.1	methionyl-tRNA formyltransferase	-
BZK09_RS06470 (BZK09_06480)	rsmB	1260753..1262060 (+)	1308	WP_000572239.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92535.44 Da
• Isoelectric Point: 6.7334

>NTDB_id=181566 BZK09_RS06445 WP_000560853.1 1254818..1257226(+) (priA) [Staphylococcus aureus strain GD1677]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKED
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQFKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRNGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=181566 BZK09_RS06445 WP_000560853.1 1254818..1257226(+) (priA) [Staphylococcus aureus strain GD1677]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCCGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTAATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCAGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGTTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGGTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTACTTATGAAGTTGTTAAAAGAGGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACTAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAATTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATTTAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATAAGGAATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGATTAATCATTATTGATGAAGAACATGAATCTACATATAAACAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGTGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATCGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGGGATTGTGGATATGTACCGCAATGCCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAACATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAATTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.433	100	0.509