Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BSA145_RS08275 Genome accession   NZ_CP015607
Coordinates   1548467..1550791 (+) Length   774 a.a.
NCBI ID   WP_075622129.1    Uniprot ID   -
Organism   Bacillus safensis strain U14-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1543467..1555791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA145_RS08250 (BSA145_08110) - 1544496..1544792 (+) 297 WP_003216788.1 post-transcriptional regulator -
  BSA145_RS08255 (BSA145_08115) secDF 1544834..1547062 (+) 2229 WP_075622127.1 protein translocase subunit SecDF -
  BSA145_RS08265 (BSA145_08120) - 1547445..1547933 (+) 489 WP_024423136.1 cation:proton antiporter regulatory subunit -
  BSA145_RS08270 (BSA145_08125) - 1548035..1548385 (+) 351 WP_041107051.1 LapA family protein -
  BSA145_RS08275 (BSA145_08130) recJ 1548467..1550791 (+) 2325 WP_075622129.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BSA145_RS08280 (BSA145_08135) - 1550815..1551327 (+) 513 WP_034279900.1 adenine phosphoribosyltransferase -
  BSA145_RS08285 (BSA145_08140) - 1551470..1553680 (+) 2211 WP_034279902.1 RelA/SpoT family protein -
  BSA145_RS08290 (BSA145_08145) dtd 1553699..1554142 (+) 444 WP_075622130.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87008.58 Da        Isoelectric Point: 4.8856

>NTDB_id=181323 BSA145_RS08275 WP_075622129.1 1548467..1550791(+) (recJ) [Bacillus safensis strain U14-5]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAALNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGELPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEASEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVSLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDTLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDLPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=181323 BSA145_RS08275 WP_075622129.1 1548467..1550791(+) (recJ) [Bacillus safensis strain U14-5]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGACCTAATGAACAAGCTTTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTCTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGAGAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACCATCGCGGATCTTGTACCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTATGCAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCAAGCGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGGCGAATTGAACAGGCAGATC
CAGCCGTTCATCTTCTGATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCAA
TTGTGCTTGGAATAGATGAAGAAACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
ATCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATTCAGCGGGTCG
ATGCCGTGTGCAAAGTAGACGAATTGACGGTACAAGCGATTGAAGAAGCGGGAATGCTGTCACCATTTGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACACGCTGCTTGACTGTGTTGGCTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATCTCCGTGATTGGGGAAATGTCCGTCAATGAATGGAATAACCGGAAAAAACCCCAGCTCATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGAGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACACTTGATTCAGAAAA
ATCATTGGTCGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATCTCCCCATTCATCATGTTCATGCAGAAAATGTAT
CCACATTTGATGTACAAGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCGAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTTAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTTGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCGTCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(311-437)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676

Multiple sequence alignment