Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BTI679_RS23025 Genome accession   NZ_CP015257
Coordinates   4417021..4419360 (-) Length   779 a.a.
NCBI ID   WP_098102379.1    Uniprot ID   A0A2A8F548
Organism   Bacillus wiedmannii strain Bt18679     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4412021..4424360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTI679_RS23005 (BTI679_46680) - 4413164..4413340 (-) 177 WP_001242605.1 hypothetical protein -
  BTI679_RS23010 (BTI679_46690) - 4413609..4414049 (-) 441 WP_001266955.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BTI679_RS23015 (BTI679_46700) relA 4414067..4416250 (-) 2184 WP_064474715.1 GTP diphosphokinase -
  BTI679_RS23020 (BTI679_46710) - 4416461..4416973 (-) 513 WP_000346221.1 adenine phosphoribosyltransferase -
  BTI679_RS23025 (BTI679_46720) recJ 4417021..4419360 (-) 2340 WP_098102379.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BTI679_RS23030 (BTI679_46730) - 4419462..4420355 (-) 894 WP_088048076.1 cation diffusion facilitator family transporter -
  BTI679_RS23035 (BTI679_46740) secDF 4420512..4422776 (-) 2265 WP_064474713.1 protein translocase subunit SecDF -
  BTI679_RS23040 (BTI679_46750) - 4423047..4423331 (-) 285 WP_000890214.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88220.80 Da        Isoelectric Point: 4.9315

>NTDB_id=178135 BTI679_RS23025 WP_098102379.1 4417021..4419360(-) (recJ) [Bacillus wiedmannii strain Bt18679]
MLQPKTRWKEKEYNNERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSLAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSEEVLNKFSLEDYKEHMMHASEVTHLDDQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=178135 BTI679_RS23025 WP_098102379.1 4417021..4419360(-) (recJ) [Bacillus wiedmannii strain Bt18679]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATAATGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCCTTGCAAGAATT
AGGTGCAGACGTAGAATTTTATATTCCAAACCGTTTTACAGAAGGATATGGACCAAACGAAGAAGCATTTCGTTGGGCAC
ATAGCGCAGGATTCTCACTAATTATCACTGTCGATACTGGTATCGCAGCAGTGCATGAAGCGAAGGTAGCGAAGGAGCTT
GGAATAGATCTTATTATTACAGATCACCATGAGCCACCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCACATGCACTACTAGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTGTCACTTCATGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAGCATATGCGCATGACGAAAAATATCGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGCATTGGATTCTCACTCGCACCACGTATTAATGCAGTTGGCCGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGATGATCCAGAAGAAGCAAAAGAGCTGGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAAATAATTTCCCGCCAGAAGACAATAAAGT
ACTAGTACTGGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTCGCATCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACAGCAAAAGGTTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACATTTCGGAGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGCTTAAATGAACAAGCAGAGGTAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CTGTTGATGTCTTTTGTAAAGTGGAGGATGTAACACTGGCAGCAATTGAAGATATGCAAAAGTTAGCGCCATTTGGTGTT
GGAAATCCAAAACCACGTATTGCAGTGAAGGATGCAGAGCTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCACATTT
GAAAATGGCTCTTCGCGATGGACAAGCGACACTGGATACAATCGGATTTGGATTTGGTACGTATGCGAAAGAAATTTCTC
CAGTTGCAAAGGTATCTGTAATAGGGGAAGCATCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCGAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACTCATTTAGACGATCAATACATCGTGTTACTTGATTTGCCAAAAGGAACAGATGAATTACGAGAT
TTATTTAAAGTGGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGAACATTTTAAATGGTACTATTCGTTCTTGAGCCAAAAAGCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A8F548

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.972

99.358

0.526

Multiple sequence alignment