Detailed information NTG0176646

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BUN12_RS04295
• Genome accession: NZ_CP018902
• Coordinates: 769891..772302 (+)
• Length: 803 a.a.
• NCBI ID: WP_013352214.1
• Uniprot ID: A0A9P1JGW2
• Organism: Bacillus amyloliquefaciens strain HK1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 764891..777302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BUN12_RS04270 (BUN12_0834)	-	766533..767408 (+)	876	WP_013352211.1	YicC/YloC family endoribonuclease	-
BUN12_RS04275 (BUN12_0835)	remA	767485..767754 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
BUN12_RS04280 (BUN12_0836)	gmk	767762..768376 (+)	615	WP_013352212.1	guanylate kinase	-
BUN12_RS04285 (BUN12_0837)	rpoZ	768380..768583 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
BUN12_RS04290 (BUN12_0838)	coaBC	768674..769894 (+)	1221	WP_013352213.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BUN12_RS04295 (BUN12_0839)	priA	769891..772302 (+)	2412	WP_013352214.1	primosomal protein N'	Machinery gene
BUN12_RS04300 (BUN12_0840)	def	772326..772808 (+)	483	WP_013352215.1	peptide deformylase	-
BUN12_RS04305 (BUN12_0841)	fmt	772813..773766 (+)	954	WP_013352216.1	methionyl-tRNA formyltransferase	-
BUN12_RS04310 (BUN12_0842)	rsmB	773753..775096 (+)	1344	WP_013352217.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
BUN12_RS04315 (BUN12_0843)	rlmN	775100..776191 (+)	1092	WP_013352218.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
BUN12_RS04320 (BUN12_0844)	-	776197..776958 (+)	762	WP_013352219.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90972.05 Da
• Isoelectric Point: 8.8944

>NTDB_id=176646 BUN12_RS04295 WP_013352214.1 769891..772302(+) (priA) [Bacillus amyloliquefaciens strain HK1]
MNVAEVIVDVSSKNIDRPFDYRIPDHLRGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGQMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIRQLFRNQKSLLYSDITDERILSALQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIDGGAKISAAELCQKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKKAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVKLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSKELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=176646 BUN12_RS04295 WP_013352214.1 769891..772302(+) (priA) [Bacillus amyloliquefaciens strain HK1]
ATGAATGTGGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGGCCCTTTGATTACAGAATTCCCGATCATTT
AAGAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTCGGCCCGCGCAAGCTTCAAGGGTTCGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGGCAAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACACCCGTTTTGACGGAAGAG
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCGGCCGC
GCTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAGGCAGCTTTTCAGAA
ATCAGAAGAGCCTTCTGTACTCAGATATTACCGATGAACGCATTCTTTCGGCACTGCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACCTATAAAGTCGCCCAGAAAACGAATAAAAAAATGATCCGCATGATTGAGGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAACAGGCTTCTAAACAGCTTGCCGTGCTTACTTATTTTATTGACGGTGGAG
CAAAGATTTCCGCGGCTGAGCTTTGCCAAAAGACCGATTCCAGTTCAGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAACCTCTCTCTCTTAC
TGAAGAGCAACAGGCCGCTTTTGAGCCGATTAAGAAGGCCGTAGCCGATCATGTTCATCACGTCTTTCTGCTTCACGGTG
TGACAGGGAGCGGCAAAACGGAAATTTACCTGCAATCTATTGAAAAGGTGCTGGCTAAAGGAAAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCATTGACGCCGCAAATGGTCAATCGTTTTAAAGGCCGCTTCGGTTCACAGGTTGCGGTCATGCACAG
CGGATTGTCCACAGGTGAAAAATACGATGAATGGCGGAAAATTCAGCGTAAGGAAGTAAAGCTTGTCGTCGGAGCCAGGT
CGGCCATCTTTGCCCCATTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCCTCCTATAAGCAGGAGGAA
ATGCCGCGGTACCATGCAAAAGACGTAGCCATACGCAGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTCGCACGGGCGAAAAAAGGCGTTTATGAGCTGCTCCCGCTGAAACACCGCGTAAATCGCCAGC
TCATGCCGGATGTATCTCTCGTTGATATGAGAGAAGAACTGCGAAACGGAAACAGATCGATGTTTTCAAAAGAGCTGATG
GAACAGCTGGAGGAGAAGCTTGCGAAAAAAGAGCAGGCCGTTCTCTTTCTGAATAAACGGGGATATTCTTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCCCAATGTCCGCATTGCGACATTTCAATGACCTATCACCGCTACGGACAAAGGCTCA
AATGCCATTACTGCGGCCATGAGGAGCCTGTTCCGGGAACGTGCCCGGAATGCGGCAGTGAGCATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTAGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACGAC
CTCAAGAAAAGGCGCTCATGAAAAGCTATTGTCAGCTTTCGGTGAAGGAAAAGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCGGCGGAGAAGACATTTCAGCTGTTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTAGT
CATACAAACGTACACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCATGACTATGAAACTTTCTTTCAGCAGGAGA
TGGCGCACAGGCGGGCCCAGTCATATCCGCCTTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCCGCAAAA
GCCGCGGTCACCGCTGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGACCGGACACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAAATTCTGGAGCATTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

3D structure

• AlphaFold DB: A0A9P1JGW2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	83.851	100	0.841