Detailed information NTG0173306

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BSF20_RS18365
• Genome accession: NZ_CP018152
• Coordinates: 3666634..3669045 (-)
• Length: 803 a.a.
• NCBI ID: WP_033574586.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain LM2303
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3661634..3674045

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSF20_RS18340 (BSF20_17980)	-	3661977..3662738 (-)	762	WP_015388247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
BSF20_RS18345 (BSF20_17985)	rlmN	3662744..3663835 (-)	1092	WP_003154339.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
BSF20_RS18350 (BSF20_17990)	rsmB	3663839..3665182 (-)	1344	WP_003154341.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
BSF20_RS18355 (BSF20_17995)	fmt	3665169..3666122 (-)	954	WP_003154342.1	methionyl-tRNA formyltransferase	-
BSF20_RS18360 (BSF20_18000)	def	3666127..3666609 (-)	483	WP_044053195.1	peptide deformylase	-
BSF20_RS18365 (BSF20_18005)	priA	3666634..3669045 (-)	2412	WP_033574586.1	primosomal protein N'	Machinery gene
BSF20_RS18370 (BSF20_18010)	coaBC	3669042..3670262 (-)	1221	WP_003154345.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BSF20_RS18375 (BSF20_18015)	rpoZ	3670353..3670556 (-)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
BSF20_RS18380 (BSF20_18020)	gmk	3670560..3671174 (-)	615	WP_003154347.1	guanylate kinase	-
BSF20_RS18385 (BSF20_18025)	remA	3671182..3671451 (-)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
BSF20_RS18390 (BSF20_18030)	-	3671528..3672403 (-)	876	WP_007409745.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90733.76 Da
• Isoelectric Point: 8.8944

>NTDB_id=173306 BSF20_RS18365 WP_033574586.1 3666634..3669045(-) (priA) [Bacillus amyloliquefaciens strain LM2303]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGSMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIGGGAKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKQAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAAHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=173306 BSF20_RS18365 WP_033574586.1 3666634..3669045(-) (priA) [Bacillus amyloliquefaciens strain LM2303]
ATGAATGTAGCAGAAGTTATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACGCCCGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCCGCCGC
ACTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCGGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAGCAGGCTTCTAAACAGCTCGCCGTGCTTACTTATTTTATCGGCGGGGGAG
CAAAGATTTCCGCGGCCGAGCTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCAGGCCGCTTTTGAGCCGATTAAACAAGCTGTAGCCGATCATGTCCATCACGTCTTTTTGCTTCACGGCG
TCACAGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTCGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGCGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAATCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTTCCGTTAAAACATCGCGTAAATCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTTTCAAGAGAGCTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCCGTCCTTTTTCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCAATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTTGGC
ACGGGCACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAGATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCAGATTTC
AGGGCCGCGGAGAAGACATTCCAGCTGCTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGACGGGCCCAGTCATACCCGCCGTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	84.224	100	0.844