Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   BMR72_RS25550 Genome accession   NZ_CP017969
Coordinates   5292444..5292869 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa isolate B10W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5287444..5297869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMR72_RS25535 (BMR72_24660) pilX 5288014..5288601 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  BMR72_RS25540 (BMR72_24665) pilY1 5288613..5292098 (+) 3486 WP_003134937.1 type 4a pilus biogenesis protein PilY1 -
  BMR72_RS25545 (BMR72_24670) pilY2 5292100..5292447 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  BMR72_RS25550 (BMR72_24675) comF 5292444..5292869 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  BMR72_RS25555 (BMR72_24680) ispH 5292916..5293860 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  BMR72_RS25560 (BMR72_24685) fkpB 5293946..5294386 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  BMR72_RS25565 (BMR72_24690) lspA 5294379..5294888 (-) 510 WP_003094728.1 signal peptidase II -
  BMR72_RS25570 (BMR72_24695) ileS 5294881..5297712 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=172275 BMR72_RS25550 WP_003094721.1 5292444..5292869(+) (comF) [Pseudomonas aeruginosa isolate B10W]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=172275 BMR72_RS25550 WP_003094721.1 5292444..5292869(+) (comF) [Pseudomonas aeruginosa isolate B10W]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCAGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTAGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment