Detailed information NTG0170692

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BKN48_RS18510
• Genome accession: NZ_CP017676
• Coordinates: 3523432..3525849 (+)
• Length: 805 a.a.
• NCBI ID: WP_070548947.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain VV2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3518432..3530849

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKN48_RS18485 (BKN48_18485)	endC	3520084..3520959 (+)	876	WP_009967215.1	YicC/YloC family endoribonuclease	-
BKN48_RS18490 (BKN48_18490)	remA	3521036..3521305 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
BKN48_RS18495 (BKN48_18495)	gmk	3521313..3521927 (+)	615	WP_070548942.1	guanylate kinase	-
BKN48_RS18500 (BKN48_18500)	rpoZ	3521931..3522134 (+)	204	WP_003221520.1	DNA-directed RNA polymerase subunit omega	-
BKN48_RS18505 (BKN48_18505)	coaBC	3522215..3523435 (+)	1221	WP_070548944.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BKN48_RS18510 (BKN48_18510)	priA	3523432..3525849 (+)	2418	WP_070548947.1	primosomal protein N'	Machinery gene
BKN48_RS18515 (BKN48_18515)	def	3525876..3526358 (+)	483	WP_015383630.1	peptide deformylase	-
BKN48_RS18520 (BKN48_18520)	fmt	3526363..3527316 (+)	954	WP_021479312.1	methionyl-tRNA formyltransferase	-
BKN48_RS18525 (BKN48_18525)	rsmB	3527303..3528646 (+)	1344	WP_021479311.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
BKN48_RS18530 (BKN48_18530)	rlmN	3528650..3529741 (+)	1092	WP_021479310.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
BKN48_RS18535 (BKN48_18535)	prpC	3529748..3530512 (+)	765	WP_014479762.1	protein-serine/threonine phosphatase PrpC	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91325.37 Da
• Isoelectric Point: 8.4336

>NTDB_id=170692 BKN48_RS18510 WP_070548947.1 3523432..3525849(+) (priA) [Bacillus subtilis strain VV2]
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMDLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHDTLKLIQRHVQKGV
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=170692 BKN48_RS18510 WP_070548947.1 3523432..3525849(+) (priA) [Bacillus subtilis strain VV2]
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
GAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCGTTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGAAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGGATCTGTCATCATGGCTGTCAGACAAAACGCTGTCTTTTAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGCACACGGCGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGATACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGTC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGGCTGAGGGATTGTCCCGACAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCAGCGGAATTGTGCAAAAAAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCAGGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTTGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACGGAAATTTATCTGCAATCAATTGAAAAAGTACTGGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCGGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCCCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCCGCGATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAGCACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAGTCATATGCAAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ACCGGGTGATGCCTGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGCTCGATGTTTTCAGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCCAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTATTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTCGGAACGGGAACACAGCGAGTGGAGGAAGAGCTGACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATTGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAAACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCCCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAGATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
TAGTGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CCGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• Helicase conserved C-terminal domain (Helicase_C) (565-662)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-545)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	99.627	100	0.996