Detailed information NTG0170176

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: AXW78_RS05540
• Genome accession: NZ_CP014282
• Coordinates: 1101874..1105599 (+)
• Length: 1241 a.a.
• NCBI ID: WP_061883887.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain Bt185
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1096874..1110599

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXW78_RS05525 (AXW78_05525)	-	1097032..1097625 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
AXW78_RS05530 (AXW78_05530)	lepB	1097682..1098245 (+)	564	WP_000751899.1	signal peptidase I	-
AXW78_RS05535 (AXW78_05535)	addB	1098362..1101877 (+)	3516	WP_061883886.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
AXW78_RS05540 (AXW78_05540)	addA	1101874..1105599 (+)	3726	WP_061883887.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
AXW78_RS05545 (AXW78_05545)	-	1105612..1105899 (+)	288	WP_000179952.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
AXW78_RS05550 (AXW78_05550)	gerPF	1106037..1106252 (-)	216	WP_001141566.1	spore germination protein GerPF	-
AXW78_RS05555 (AXW78_05555)	-	1106295..1106681 (-)	387	WP_061883888.1	spore germination protein GerPE	-
AXW78_RS05560 (AXW78_05560)	gerPD	1106697..1106891 (-)	195	WP_001052806.1	spore germination protein GerPD	-
AXW78_RS05565 (AXW78_05565)	gerPC	1106898..1107512 (-)	615	WP_001070760.1	spore germination protein GerPC	-
AXW78_RS05570 (AXW78_05570)	gerPB	1107580..1107786 (-)	207	WP_001012516.1	spore germination protein GerPB	-
AXW78_RS05575 (AXW78_05575)	gerPA	1107801..1108022 (-)	222	WP_001111188.1	spore germination protein GerPA	-
AXW78_RS05580 (AXW78_05580)	-	1108119..1108298 (-)	180	WP_000462841.1	aspartyl-phosphate phosphatase Spo0E family protein	-
AXW78_RS05585 (AXW78_05585)	-	1108546..1109445 (+)	900	WP_061883889.1	fumarylacetoacetate hydrolase family protein	-
AXW78_RS05590 (AXW78_05590)	-	1109481..1109765 (-)	285	WP_000926865.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142699.04 Da
• Isoelectric Point: 4.8313

>NTDB_id=170176 AXW78_RS05540 WP_061883887.1 1101874..1105599(+) (addA) [Bacillus thuringiensis strain Bt185]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESKAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGTLSSAARESWTSLYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEPSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESAGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDVDAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGSEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELAMLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHIVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=170176 AXW78_RS05540 WP_061883887.1 1101874..1105599(+) (addA) [Bacillus thuringiensis strain Bt185]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCTATTTCGACGATTCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAAAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACGGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACGTTGCAAGCAGATGCAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAATCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACCAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAATTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGCAGGGAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AACCAGGACTATTCCTAGGAAAATATAAACGCTTCACACAAGAAGGATTGGGTGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAATTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGTTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTTCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTATATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATATGCTGACCTCGCGACTGGTTACTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCGATCGTTGGATTAAACGATGAAGAACTTGCAATGCTTCG
TGCTCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAACTTACTACAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTTGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGCAAACGAATTAAATATACAACATTAT
CACAGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTGACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAATACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGCGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCCATTGAAAAAGTAATTTCATTCTTCGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGAAAATTCCCAGGCGGATTTGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATATTGTAAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.408	100	0.537