Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   AS852_RS08255 Genome accession   NZ_CP013218
Coordinates   1637809..1638879 (-) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus strain LA-MRSA ST398 isolate E154     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632809..1643879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS852_RS08215 (AS852_07920) gcvPA 1632987..1634333 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AS852_RS08220 (AS852_07925) gcvT 1634353..1635444 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AS852_RS08225 (AS852_07930) - 1635603..1636127 (-) 525 WP_001015120.1 shikimate kinase -
  AS852_RS08230 (AS852_07935) - 1636117..1636263 (-) 147 WP_001789879.1 hypothetical protein -
  AS852_RS08235 (AS852_07940) comGF 1636360..1636857 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  AS852_RS08240 (AS852_07945) comGE 1636775..1637074 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AS852_RS08245 (AS852_07950) comGD 1637061..1637507 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  AS852_RS08250 (AS852_07955) comGC 1637485..1637795 (-) 311 Protein_1547 competence type IV pilus major pilin ComGC -
  AS852_RS08255 (AS852_07960) comGB 1637809..1638879 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  AS852_RS08260 (AS852_07965) comGA 1638851..1639825 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  AS852_RS08265 (AS852_07970) - 1639877..1640500 (-) 624 WP_031919558.1 MBL fold metallo-hydrolase -
  AS852_RS08270 (AS852_07975) - 1640497..1640826 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AS852_RS08275 (AS852_07980) - 1640826..1641812 (-) 987 WP_000161315.1 ROK family glucokinase -
  AS852_RS08280 (AS852_07985) - 1641809..1642012 (-) 204 WP_000087559.1 YqgQ family protein -
  AS852_RS08285 (AS852_07990) - 1641993..1643456 (-) 1464 WP_001017856.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=160791 AS852_RS08255 WP_000776425.1 1637809..1638879(-) (comGB) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=160791 AS852_RS08255 WP_000776425.1 1637809..1638879(-) (comGB) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment