Detailed information NTG0157603

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: ACH32_RS00945
• Genome accession: NZ_CP012756
• Coordinates: 175987..178464 (-)
• Length: 825 a.a.
• NCBI ID: WP_060649587.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain JS395
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 170987..183464

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACH32_RS00920 (ACH32_00915)	-	171012..171650 (-)	639	WP_000342269.1	O-methyltransferase	-
ACH32_RS00925 (ACH32_00920)	-	171936..172244 (-)	309	WP_000134779.1	DUF1292 domain-containing protein	-
ACH32_RS00930 (ACH32_00925)	ruvX	172259..172687 (-)	429	WP_000939059.1	Holliday junction resolvase RuvX	-
ACH32_RS00935 (ACH32_00930)	-	172691..172951 (-)	261	WP_060649586.1	IreB family regulatory phosphoprotein	-
ACH32_RS00940 (ACH32_00935)	alaS	173014..175644 (-)	2631	WP_000734069.1	alanine--tRNA ligase	-
ACH32_RS00945 (ACH32_00940)	recD2	175987..178464 (-)	2478	WP_060649587.1	ATP-dependent RecD-like DNA helicase	Machinery gene
ACH32_RS00950 (ACH32_00945)	-	178466..179134 (-)	669	WP_000567023.1	tetratricopeptide repeat protein	-
ACH32_RS00955 (ACH32_00950)	mnmA	179767..180885 (-)	1119	WP_000066103.1	tRNA 2-thiouridine(34) synthase MnmA	-
ACH32_RS00960 (ACH32_00955)	-	180886..182028 (-)	1143	WP_000409173.1	cysteine desulfurase family protein	-
ACH32_RS00965 (ACH32_00960)	-	182340..183353 (+)	1014	WP_060649588.1	LLM class flavin-dependent oxidoreductase	-

Sequence

Protein

• Length: 825 a.a.
• Molecular weight: 93071.88 Da
• Isoelectric Point: 4.5154

>NTDB_id=157603 ACH32_RS00945 WP_060649587.1 175987..178464(-) (recD2) [Staphylococcus aureus strain JS395]
MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDTMPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAE
TFEKEMPQMKEAIISYLSSDLFKGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAYNDNERLKAALLYTLEEECIKQG
HTYLSINVVIDLTVDVLNYQDEEVIEPEKLDEMLQYLNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIE
QSDLQMHIGEIEDTNQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVNDDYPVVLA
APTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDEMSMVDTWLFHQFLSAVPLDAQLIFVGDEDQ
LPSVGPGQVFKDLIESKAIPRVNLTEVYRQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAV
NKGYTMADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPNDNIFNGDIGVIVGIFW
AKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQGSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLL
GDPEAFDIGLKTNGRARLTQLCTLLKNYFNSEDEEILENTATNDTGASQTTINDQVEAPISSNDSEEVTSDSIKTDTNVL
TEATMFKIDPMINMGEITPYDFIER

Nucleotide

• Length: 2478 bp

>NTDB_id=157603 ACH32_RS00945 WP_060649587.1 175987..178464(-) (recD2) [Staphylococcus aureus strain JS395]
ATGTCAGACCCTACACTATTTGATTATTCAATGATCAAAGGTACTGTTGAAGCGATATTGTTTCAAAACAGTGATAATTT
TTATACAGTGCTTAAAGTAGATACTATTGAAACAAATGAAGACTTTGATACAATGCCAACTGTTGTAGGGTTTCTTCCGA
ATATTGTTGAAGGCGATGTCTACACATTTAAAGGGCAAGTTGTTGATCATCCACGTTATGGTAAACAATTAAAAGCAGAG
ACTTTTGAAAAAGAAATGCCACAAATGAAAGAAGCAATTATAAGTTATTTATCAAGTGATTTGTTTAAAGGTGTAGGTAA
AAAGACAGCTCAAAATATTGTTAATACATTAGGTGATAATGCGATAAATGATATATTAGATGACCATTCAGTGCTAGAAA
AAGTTTCGGGATTATCTAAAAAGAAACAGAAACAAATTGCAGAACAAATTTCAGCAAATCAAGAATCTGAAAAAATAATG
ATTCGTTTACATGATTTAGGGTTTGGTCCTAAACTTTCAATGGCAATTTATCAGTTTTATCTCGGTGATACACTGACTAT
TTTAGATCGAAATCCATATCAATTGATTTATGATATTAAAGGTATTGGTTTTAATAAAGCAGATCAACTTGCTAGAAATA
TTGGTATCGCTTATAACGATAATGAACGATTAAAAGCTGCGTTGCTCTATACGTTAGAAGAAGAATGTATTAAACAGGGA
CATACATATTTGTCTATCAATGTTGTTATAGACTTAACCGTTGATGTATTAAATTATCAAGATGAAGAGGTCATTGAACC
TGAAAAATTAGACGAAATGTTGCAATACTTAAATGAGGAAAAACGACTAATTATTGACAATGAACAAGTTGCAATTCCAA
GTTTGTATTATTCTGAAATCAAAAGTGTTCAAAACTTATTCAGAATAAAGACGCATACAAATAAACTTACGGAAATTGAA
CAGTCAGATTTACAAATGCATATTGGTGAAATAGAAGATACGAATCAAGTGAACTATGCAGCTTCACAAAAAGAAGCTTT
GCAAACGGCTATCAATTCTAAGGTGATGCTATTAACCGGTGGACCGGGAACAGGAAAGACGACAGTAATTAAAGGCATTG
TAGAATTATATGCTGAGATTCATGGCTTATCATTAGATTATGATGATTATGTTAATGATGATTATCCAGTTGTATTAGCA
GCGCCAACAGGAAGAGCGTCCAAGAGACTACAAGAGTCAACTGGATTAGAAGCTATGACGATTCATCGTTTAATAGGTTG
GAATCAAGACACAAAGCCAGAGGATATATTAGAAAATGAAATAAACGCACGACTCATAATCATTGATGAGATGTCTATGG
TAGATACTTGGTTGTTCCACCAATTTTTAAGTGCAGTTCCTTTAGATGCACAATTGATATTTGTAGGTGATGAGGACCAA
TTGCCTTCTGTAGGTCCTGGTCAAGTATTTAAAGATTTAATTGAATCGAAAGCTATACCACGAGTTAATCTGACTGAAGT
ATATCGTCAACAAGATGGTTCAAGCATTATAGAATTAGCACATCGAATGAAGTTGGGTCAGAAAATTGATATTACACAGC
GTTTTCATGATAGAAGTTTTATTAACTGTCAGGCAAACCAAATACCAACAGTTGTTGAGAAGGTAGTTACTAGTGCTGTT
AATAAAGGGTATACTATGGCTGATATTCAAGTGCTTGCACCAATGTATAAAGGAAATGCCGGTATTAAACGTTTAAATCA
AGTTTTACAAGATATTTTAAACCCTAAAAAGAAAGACACACGAGAAATTGAGTTTGGTGATGTAGTTTTTAGAAAAGGTG
ACAAAGTATTACAACTTGTTAATAGGCCAAATGATAACATATTCAATGGTGACATAGGAGTTATTGTTGGTATTTTTTGG
GCGAAAGAAAATGCTCTTAATAAAGATGTACTTGTTGTGGATTTTGAAGGTAATGAAATTACATTTACGAAACAAGATAT
GATGGAATTAACGCATGCATATTGTACATCAATCCATAAATCACAAGGTTCAGAATTTCCTATTGTTATCATGCCAATTG
TTAAACAGTATTTTAGAATGTTACAAAGACCGATATTATATACTGGTTTAACTAGAGCGAAAACGAGTTTAGTTTTACTT
GGTGATCCAGAAGCATTTGATATTGGATTAAAAACAAATGGTCGAGCGCGTTTAACACAATTATGTACATTGTTGAAAAA
TTATTTTAATTCAGAAGATGAAGAAATACTGGAAAATACAGCAACTAATGATACAGGAGCTTCACAAACGACAATAAATG
ATCAAGTAGAAGCGCCAATTTCATCGAATGATAGTGAAGAAGTTACGTCTGATTCAATAAAAACAGATACAAATGTATTA
ACGGAAGCTACAATGTTTAAAATTGATCCAATGATTAATATGGGTGAAATCACGCCGTATGACTTTATAGAACGTTGA

Domains

Predicted by InterproScan.

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (585-655)

• AAA domain (AAA_30) (339-544)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (672-720)

• Helix-hairpin-helix containing domain (HHH_RecD2) (153-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	52.722	91.273	0.481