Detailed information NTG0156174

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: LLUC77_RS10740
• Genome accession: NZ_CP015906
• Coordinates: 2088200..2090539 (-)
• Length: 779 a.a.
• NCBI ID: WP_003131470.1
• Uniprot ID: Q9CEE5
• Organism: Lactococcus lactis subsp. lactis strain UC77
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2083200..2095539

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC77_RS10700 (LLUC77_2047)	-	2084116..2084622 (-)	507	WP_003131479.1	Asp23/Gls24 family envelope stress response protein	-
LLUC77_RS10705 (LLUC77_2048)	-	2084643..2084831 (-)	189	WP_012898445.1	DUF2273 domain-containing protein	-
LLUC77_RS10710 (LLUC77_2049)	amaP	2084842..2085402 (-)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
LLUC77_RS10715 (LLUC77_2050)	-	2085430..2085672 (-)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
LLUC77_RS10720 (LLUC77_2051)	fmt	2085847..2086806 (-)	960	WP_010906187.1	methionyl-tRNA formyltransferase	-
LLUC77_RS10725 (LLUC77_2052)	-	2086803..2087267 (-)	465	WP_003131474.1	VOC family protein	-
LLUC77_RS10730 (LLUC77_2053)	-	2087233..2087754 (-)	522	WP_003131472.1	NUDIX hydrolase	-
LLUC77_RS10735 (LLUC77_2054)	-	2087767..2088141 (-)	375	WP_010906188.1	nuclear transport factor 2 family protein	-
LLUC77_RS10740 (LLUC77_2055)	priA	2088200..2090539 (-)	2340	WP_003131470.1	primosomal protein N'	Machinery gene
LLUC77_RS10745 (LLUC77_2056)	rpoZ	2090678..2091031 (-)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
LLUC77_RS10750 (LLUC77_2057)	gmk	2091211..2091828 (-)	618	WP_003131468.1	guanylate kinase	-
LLUC77_RS10755 (LLUC77_2058)	rny	2091976..2093571 (-)	1596	WP_003131467.1	ribonuclease Y	-
LLUC77_RS10760 (LLUC77_2059)	-	2093802..2094515 (-)	714	WP_003131466.1	glucosamine-6-phosphate deaminase	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88929.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=156174 LLUC77_RS10740 WP_003131470.1 2088200..2090539(-) (priA) [Lactococcus lactis subsp. lactis strain UC77]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=156174 LLUC77_RS10740 WP_003131470.1 2088200..2090539(-) (priA) [Lactococcus lactis subsp. lactis strain UC77]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAAA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCTGTCAGATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAAGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCTGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Type III restriction enzyme, res subunit (ResIII) (247-405)

3D structure

• AlphaFold DB: Q9CEE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.637	100	0.492