Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A7317_RS28025 Genome accession   NZ_CP015637
Coordinates   6258936..6260615 (+) Length   559 a.a.
NCBI ID   WP_069077240.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain L321     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6253936..6265615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7317_RS28005 (A7317_27975) argE 6255209..6256357 (-) 1149 WP_024077986.1 acetylornithine deacetylase -
  A7317_RS28010 (A7317_27980) - 6256516..6257883 (+) 1368 WP_024077987.1 inorganic triphosphatase -
  A7317_RS28015 (A7317_27985) - 6257923..6258393 (-) 471 WP_024077988.1 Lrp/AsnC family transcriptional regulator -
  A7317_RS28020 (A7317_27990) - 6258521..6258859 (+) 339 WP_024077989.1 hypothetical protein -
  A7317_RS28025 (A7317_27995) pilB 6258936..6260615 (+) 1680 WP_069077240.1 GspE/PulE family protein Machinery gene
  A7317_RS28030 (A7317_28000) - 6260693..6261613 (+) 921 WP_024077991.1 SPFH domain-containing protein -
  A7317_RS28035 (A7317_28005) - 6261624..6262070 (+) 447 WP_024077992.1 NfeD family protein -
  A7317_RS28040 (A7317_28010) - 6262140..6262457 (+) 318 WP_041161049.1 DUF2388 domain-containing protein -
  A7317_RS28045 (A7317_28015) - 6262648..6263313 (+) 666 WP_069077241.1 outer membrane protein -
  A7317_RS28050 (A7317_28020) - 6263472..6264611 (+) 1140 WP_069077242.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  A7317_RS28055 (A7317_28025) gcvH 6264654..6265040 (-) 387 WP_069077243.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61380.06 Da        Isoelectric Point: 7.5382

>NTDB_id=154292 A7317_RS28025 WP_069077240.1 6258936..6260615(+) (pilB) [Pseudomonas fluorescens strain L321]
MPVDHRLELHPLLPALLEEGLITPDMAQRLATLPASDTQHPLEHIAALGPCLETLTQWLARHTGQAYLRIDPLKIDVATV
VPLMSHAFAQRHAILAVAVDAQSVTIASAQPHVTAWEAGLAQVLKRSIKRVVANPQDIQRCIAQFYRLAKSVSGADLKMA
APGHFDLLNLGIGDQEPDANDAHIVNIVDWLLQYAFGQRASDIHVEPCRDQGRVRFRIDGLLHDVYQFPSQVTVAVVSRL
KSLGRMNVAEKRKPQDGRVKTKSPAGAEVELRLSTLPTAFGEKLVMRIFDPQVLLKDFDQLGLPTEDQRRWHTMVRQTSG
IILVTGPTGSGKTSTLYTTLKQLATREVNLCTVEDPIEMVEPAFNQMQVQSNIDLTFASGIRALLRQDPDIIMIGEIRDR
ETAEMAIQAALTGHLVLSTLHTNDAPSAISRLQELGIAHYLIKATLIGVMAQRLVRLLCPHCKRVAGERYEAVGCAQCRD
SGYRGRAGVYEIMVMDETLKALITPGADVQALRRAALKQGMCSLRIAGLRKVNAGLTTLAEVLRVTPGDSDTNPLLVEP

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=154292 A7317_RS28025 WP_069077240.1 6258936..6260615(+) (pilB) [Pseudomonas fluorescens strain L321]
ATGCCCGTTGATCATCGATTGGAATTGCACCCGTTGTTGCCGGCCCTGCTCGAAGAAGGGCTGATCACGCCCGACATGGC
ACAGCGCCTGGCGACGCTGCCGGCCAGCGACACCCAGCACCCACTGGAACACATCGCGGCCCTGGGCCCTTGCCTGGAAA
CCCTGACCCAATGGCTTGCCCGACACACCGGGCAAGCCTACCTGCGTATCGACCCCCTGAAAATCGACGTGGCAACCGTG
GTGCCGCTGATGTCCCACGCCTTTGCCCAACGCCACGCCATCCTGGCGGTGGCCGTGGACGCGCAGAGCGTCACCATCGC
CAGCGCCCAACCCCATGTCACCGCCTGGGAAGCCGGGCTGGCGCAGGTGCTCAAGCGCTCGATCAAGCGGGTGGTCGCCA
ATCCCCAGGATATCCAGCGTTGTATCGCGCAGTTCTACCGGCTGGCCAAGTCGGTCAGCGGCGCGGATCTCAAGATGGCA
GCGCCGGGCCATTTCGACCTGCTCAACCTCGGCATCGGCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCAACAT
TGTCGACTGGCTGCTGCAGTACGCGTTCGGCCAACGCGCCAGTGATATCCATGTCGAACCGTGCCGGGACCAGGGCCGGG
TGCGCTTTCGCATCGATGGGTTGCTGCATGATGTCTATCAATTTCCGTCCCAGGTAACGGTGGCCGTGGTCAGCCGCCTG
AAAAGCCTCGGACGGATGAACGTGGCGGAGAAACGCAAACCCCAGGACGGCCGGGTCAAGACCAAAAGCCCGGCGGGCGC
CGAAGTGGAGTTGCGCCTGTCGACCTTGCCCACGGCGTTCGGCGAGAAACTGGTGATGCGGATTTTCGACCCCCAGGTGC
TACTCAAGGACTTCGATCAGTTGGGCTTGCCCACCGAAGACCAGCGGCGCTGGCACACCATGGTTCGCCAGACCAGTGGC
ATCATCCTGGTCACCGGCCCTACCGGCTCGGGCAAGACCAGCACCCTTTACACCACGCTCAAGCAACTGGCGACCCGCGA
GGTCAACCTGTGCACGGTGGAAGACCCCATCGAGATGGTCGAGCCGGCGTTCAACCAGATGCAGGTGCAGTCCAATATCG
ACCTGACCTTCGCCAGCGGTATCCGTGCCCTGCTGCGCCAGGACCCGGACATCATCATGATCGGCGAGATTCGCGACAGG
GAAACCGCCGAAATGGCGATTCAAGCTGCATTGACGGGGCACCTGGTACTGTCGACGCTGCACACCAACGATGCACCCAG
CGCTATCAGCCGTTTACAGGAACTAGGAATTGCTCATTATTTGATCAAAGCTACGCTGATTGGAGTGATGGCGCAGCGCC
TGGTGCGGCTGTTGTGCCCGCACTGCAAGCGTGTCGCGGGTGAGCGCTACGAAGCTGTGGGCTGCGCGCAGTGCCGAGAC
AGCGGTTACCGAGGCAGGGCCGGCGTGTACGAAATCATGGTCATGGACGAAACGCTCAAAGCGTTGATCACCCCCGGCGC
CGATGTGCAGGCCTTGCGCCGGGCCGCGCTCAAACAAGGCATGTGCAGCCTGCGTATCGCCGGCCTGCGCAAGGTCAACG
CCGGGCTGACCACACTGGCTGAAGTATTACGAGTAACGCCCGGGGATTCAGATACAAATCCTTTGCTTGTCGAACCGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.045

100

0.39