Detailed information NTG0153944

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: amyaer_RS13150
• Genome accession: NZ_CP012375
• Coordinates: 2674048..2675163 (-)
• Length: 371 a.a.
• NCBI ID: WP_046662421.1
• Uniprot ID: A0A0F6U5B9
• Organism: Microcystis aeruginosa NIES-2481
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2669048..2680163

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
amyaer_RS13120 (amyaer_2715)	-	2669181..2669465 (-)	285	WP_046662417.1	hypothetical protein	-
amyaer_RS13125 (amyaer_2716)	-	2669468..2669686 (+)	219	WP_046662418.1	hypothetical protein	-
amyaer_RS13135 (amyaer_2718)	-	2670205..2672295 (-)	2091	WP_046663768.1	secretin N-terminal domain-containing protein	-
amyaer_RS13140 (amyaer_2719)	-	2672480..2673271 (-)	792	WP_046662419.1	hypothetical protein	-
amyaer_RS13145 (amyaer_2720)	-	2673268..2674038 (-)	771	WP_046662420.1	PilN domain-containing protein	-
amyaer_RS13150 (amyaer_2721)	pilM	2674048..2675163 (-)	1116	WP_046662421.1	type IV pilus assembly protein PilM	Machinery gene
amyaer_RS13160 (amyaer_2722)	ilvC	2675669..2676661 (+)	993	WP_046662422.1	ketol-acid reductoisomerase	-
amyaer_RS13165 (amyaer_2723)	-	2676767..2677555 (-)	789	WP_046662423.1	ECF transporter S component	-
amyaer_RS13170 (amyaer_2724)	-	2677702..2678046 (+)	345	WP_052734183.1	transposase	-
amyaer_RS13175 (amyaer_2725)	-	2678250..2679296 (-)	1047	WP_046662424.1	LD-carboxypeptidase	-
amyaer_RS23370	-	2679432..2679503 (-)	72	Protein_2526	polyhydroxyalkanoate biosynthesis repressor PhaR	-
amyaer_RS13180 (amyaer_2727)	-	2679546..2680013 (-)	468	WP_046662425.1	CBS domain-containing protein	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41346.32 Da
• Isoelectric Point: 4.3560

>NTDB_id=153944 amyaer_RS13150 WP_046662421.1 2674048..2675163(-) (pilM) [Microcystis aeruginosa NIES-2481]
MVSFFKKLLTKKTQGVGLEITPERINVAQIAKQKQNYKLVKYCSSDIPEGIFEEGKIVDSAALAELIQEVLRENKITSKR
VATGVPMRESIIRIIAIPSELDEEELRDLVLNHEANLYLPYPREEVDIDYQKLGYFQDEDGIEKVQVLLVATRREITDAY
LETLQQAGLQVDVLEINSFALIRTIRDQLRQFSSNEATVIVDIEFDNTEIAIVVDGVPQFSRTVPIGTFQLQNALSRAMN
LPTSRSPEILLGMTIPTTAFDSLGTNTSVSRSFTTAGMAALMRVLGELTDELRRSINFYLNQSDELEIVQLLLAGPGGGL
AQLDEYFTQRLSITTMPIDPIASLNLDIDQEIPNTQRPGLGTVLGLGLREV

Nucleotide

• Length: 1116 bp

>NTDB_id=153944 amyaer_RS13150 WP_046662421.1 2674048..2675163(-) (pilM) [Microcystis aeruginosa NIES-2481]
ATGGTAAGTTTTTTTAAAAAGCTATTGACAAAAAAAACTCAAGGTGTTGGGTTAGAGATTACCCCAGAGCGCATTAACGT
GGCTCAGATCGCCAAACAGAAACAAAATTATAAATTAGTTAAATATTGTTCGTCGGATATCCCAGAAGGCATCTTTGAGG
AGGGAAAAATCGTCGATTCCGCCGCTTTAGCGGAATTAATCCAAGAAGTCCTCAGGGAAAATAAAATTACTAGCAAACGA
GTCGCTACTGGTGTCCCCATGCGCGAGTCAATTATCCGGATTATCGCCATTCCCTCGGAATTGGACGAGGAGGAATTACG
CGATCTGGTCTTAAATCATGAAGCTAATTTATACCTTCCCTATCCGCGTGAGGAAGTGGATATCGACTACCAAAAACTGG
GCTACTTTCAGGACGAGGACGGGATCGAAAAGGTGCAGGTTTTGCTGGTAGCTACTCGTCGGGAGATCACCGATGCCTAT
CTGGAAACCCTGCAACAGGCAGGATTACAGGTAGATGTGCTAGAAATTAATAGTTTTGCCCTAATTCGGACGATTCGCGA
TCAGTTGCGACAGTTTAGTTCCAATGAAGCTACCGTTATTGTCGATATAGAATTTGATAACACGGAAATCGCCATCGTGG
TGGACGGCGTTCCCCAATTTTCCCGCACTGTCCCCATCGGCACTTTCCAGCTACAAAATGCCCTTTCCAGGGCGATGAAT
TTGCCCACCTCCCGCAGTCCTGAGATTCTTTTGGGGATGACAATCCCGACTACTGCCTTTGATAGTCTTGGCACCAATAC
CAGTGTTTCCAGAAGCTTTACTACCGCCGGGATGGCGGCACTGATGCGGGTTTTGGGGGAATTAACCGATGAATTGCGGC
GATCGATTAATTTCTATCTTAACCAAAGTGATGAACTGGAAATTGTCCAGTTACTTTTAGCAGGTCCCGGGGGCGGTTTA
GCACAACTAGATGAATATTTTACTCAACGTCTTAGCATCACTACTATGCCAATCGATCCGATCGCATCACTGAACCTAGA
TATCGATCAAGAGATTCCTAATACCCAAAGACCTGGTTTAGGAACAGTTCTCGGTTTAGGATTGCGGGAGGTATAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-242)

3D structure

• AlphaFold DB: A0A0F6U5B9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Synechocystis sp. PCC 6803	73.784	99.73	0.736