Detailed information NTG0150253

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BaDB11_RS21350
• Genome accession: NZ_CP014795
• Coordinates: 4104768..4107185 (+)
• Length: 805 a.a.
• NCBI ID: WP_011197970.1
• Uniprot ID: Q65JS7
• Organism: Bacillus licheniformis strain SCK B11
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 4099768..4112185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BaDB11_RS21325 (BaDB11_04294)	-	4101427..4102302 (+)	876	WP_003181655.1	YicC/YloC family endoribonuclease	-
BaDB11_RS21330 (BaDB11_04295)	remA	4102378..4102647 (+)	270	WP_003181657.1	extracellular matrix/biofilm regulator RemA	-
BaDB11_RS21335 (BaDB11_04296)	gmk	4102655..4103269 (+)	615	WP_003181659.1	guanylate kinase	-
BaDB11_RS21340 (BaDB11_04297)	rpoZ	4103272..4103472 (+)	201	WP_003181661.1	DNA-directed RNA polymerase subunit omega	-
BaDB11_RS21345 (BaDB11_04298)	coaBC	4103554..4104771 (+)	1218	WP_003181663.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BaDB11_RS21350 (BaDB11_04299)	priA	4104768..4107185 (+)	2418	WP_011197970.1	primosomal protein N'	Machinery gene
BaDB11_RS21355 (BaDB11_04300)	def	4107215..4107697 (+)	483	WP_009328532.1	peptide deformylase	-
BaDB11_RS21360 (BaDB11_04301)	fmt	4107702..4108652 (+)	951	WP_003181670.1	methionyl-tRNA formyltransferase	-
BaDB11_RS21365 (BaDB11_04302)	rsmB	4108642..4109988 (+)	1347	WP_003181671.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
BaDB11_RS21370 (BaDB11_04303)	rlmN	4109988..4111073 (+)	1086	WP_080618638.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
BaDB11_RS21375 (BaDB11_04304)	-	4111081..4111845 (+)	765	WP_003181675.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91315.68 Da
• Isoelectric Point: 9.0887

>NTDB_id=150253 BaDB11_RS21350 WP_011197970.1 4104768..4107185(+) (priA) [Bacillus licheniformis strain SCK B11]
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELAGRSVKDIEDILDLAPVLTEE
LLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIGDKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGN
LEVAYKVKQKTNKKMRRMIAPLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSGKTEVYLQSIEKVLEAGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQ
EEMPRYHAKEAAIQRAKYHRCPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYCGYETGMPSSCPECGSEHIRY
FGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLTSFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIP
DFRAAEKTFQLMTQVSGRAGRHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNIMDHYKRDIEQKQLAISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=150253 BaDB11_RS21350 WP_011197970.1 4104768..4107185(+) (priA) [Bacillus licheniformis strain SCK B11]
ATGAACTTCGCTGAAGTGATCGTCGATGTTACAACGAAAAATATCGACAGACCGTTTGATTACCGCATCCCCGACAAATT
CAAAGGTATGATTAAAAAAGGGATGCGCGTGATCGTTCCGTTCGGCGCCCGCAAAATCCAGGGATTTGTCACCAGCGTGA
AAGAATCGTCGGAGCTTGCCGGCCGTTCGGTTAAGGATATCGAGGATATCCTTGATCTGGCGCCCGTTTTGACGGAGGAG
CTGCTTGAGCTGTCAGACTGGCTGACAGAAAAAACACTTTCTTTTAAAATTACGGCTTTGCAGGCGATGCTCCCCGCGGC
TATGAAAGCAAAATACGGCAAAGAAATCAAGGTGATCGGCGATAAACCGCTTCCCGAACCGCTAAAAACCTTGTTCTCGC
AAAAAGAGACGCTTTCTTATTCCGATATAAATGACCACGGAACATTAAAGCTGATTCAGCGCCAGGTTCAAGAAGGCAAT
CTTGAAGTGGCGTATAAAGTAAAGCAGAAAACAAACAAAAAAATGCGGAGAATGATCGCCCCCCTTTGCGCGAAAGAACA
TTTAAAAGAAGCGCTTGCCGGCCTCTCTCCGCAAGCTGTCAAACAAAAAGCGATCCTTCAGTTTTTCATCGACCGCAAAG
AGGGCGGCGCTGTTCCGGCAAAGGATCTGATCGACAAAACGGGGGGCTCTTCCGCGTCCTTGAAAGCGCTCATCGACAAG
GGGCTGTTAAAGGAAAGCGAAGAGGAGGTTTACAGGGATCCTTATCAGGACAGAATGTTCAAAAAAACCGAACCGCTTCC
GCTGACTGAAGAGCAGGCGCAGGCCTACGAGGCGATAGCGGATGCAATCGAAGAAAAGCGGCACGATGTGTTTCTTCTTC
ACGGAGTGACCGGAAGCGGAAAAACGGAAGTCTATCTGCAATCGATCGAAAAGGTGCTGGAAGCCGGCAAAGAAGCGATC
GTTCTCGTTCCGGAAATTTCCCTGACGCCGCAGATGGTCAACCGCTTTAAAGGGCGCTTTGGCTCGCAGGTAGCCGTACT
GCACAGCGGGCTTTCAACAGGAGAAAAATATGATGAATGGCGGAAAATCCACCGCAAAGAAGTCCGGCTTGTCGTCGGGG
CGCGCTCGGCGATTTTCGCGCCGTTTGAAAATCTCGGCATGATTATAATTGATGAAGAGCACGAAACATCGTACAAGCAG
GAGGAGATGCCGAGATACCATGCGAAAGAAGCGGCGATTCAAAGGGCCAAATACCACCGGTGTCCGGTCGTGCTCGGCAG
TGCGACGCCGACGCTCGAATCGTTCGCGAGGGCGAAAAAAGGCGTGTACAAGCTTCTTTCTCTCAAGCACCGCGTCAATC
ACCAAGTCATGCCCGACGTGTCTCTCGTCGATATGAGAGAGGAACTGCGCAGCGGAAACCGCTCGATGTTTTCCAAAGAC
TTAATGGCCGGGCTTGAGGAAACTCTTGAAAAAGGAGAGCAGGCCGTTCTGTTTTTAAACAAAAGAGGCTATTCCTCATT
TGTCATGTGCAGGGACTGCGGATATGTCATCCAGTGTCCTCATTGTGAAATTTCATTGACGTACCACAGGTATGGACAGC
GTCTGAAGTGCCATTACTGCGGCTATGAAACCGGCATGCCGTCAAGCTGCCCCGAATGCGGAAGCGAGCATATCCGCTAC
TTTGGTACAGGCACACAGCGGGTCGAAGAAGAATTGACGAAGGTTCTGCCGGCTGCAAGGGTGATCCGCATGGATGTCGA
TACAACCTCAAGAAAGGGAGCGCATGAAAAGCTGCTCACATCCTTTGGCAGAGGCGAAGCCGATATCCTGCTTGGAACGC
AGATGATCGCCAAAGGGCTCGATTTCGAAAACGTCACGCTCGTCGGTGTCTTGAGCGCGGATACGATGCTTCACATCCCC
GATTTCAGAGCCGCTGAAAAAACGTTCCAGCTGATGACGCAGGTGAGCGGAAGAGCGGGGCGCCACGAAAAGCCGGGCAA
GGTGATCATTCAGACGTACACACCGTCCCATTACAGCATTCAGCTCACAAAAACGCATGATTATGAAACATTCTTCCGGC
AGGAGATGCTGCACAGAAGGGAACAGGCCTATCCTCCATATTATTATTTGGCGCTGGTCAGCGTATCGCACGAGGAAGTG
ACGAAGGCGGCACTCGTGACTGAAAAAATCGCCACCTATTTAAAAAAGAACATTTCAAGCGAGGCGAAGATTCTCGGCCC
TGTCGCATCTCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAAACCAGCTTA
CATCGTTGCTTAAAAACATTATGGATCATTACAAACGAGACATTGAACAAAAGCAATTGGCGATTTCGATCGACATGAAT
CCATATATGATGATGTAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Helicase conserved C-terminal domain (Helicase_C) (578-662)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-543)

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

3D structure

• AlphaFold DB: Q65JS7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	79.006	100	0.79