Detailed information NTG0149840

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: B34_RS00635
• Genome accession: NZ_CP014793
• Coordinates: 127587..130004 (+)
• Length: 805 a.a.
• NCBI ID: WP_011197970.1
• Uniprot ID: Q65JS7
• Organism: Bacillus licheniformis strain SCDB 34
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 122587..135004

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B34_RS00610 (B34_00126)	-	124246..125121 (+)	876	WP_003181655.1	YicC/YloC family endoribonuclease	-
B34_RS00615 (B34_00127)	remA	125197..125466 (+)	270	WP_003181657.1	extracellular matrix/biofilm regulator RemA	-
B34_RS00620 (B34_00128)	gmk	125474..126088 (+)	615	WP_003181659.1	guanylate kinase	-
B34_RS00625 (B34_00129)	rpoZ	126091..126291 (+)	201	WP_003181661.1	DNA-directed RNA polymerase subunit omega	-
B34_RS00630 (B34_00130)	coaBC	126373..127590 (+)	1218	WP_003181663.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
B34_RS00635 (B34_00131)	priA	127587..130004 (+)	2418	WP_011197970.1	primosomal protein N'	Machinery gene
B34_RS00640 (B34_00132)	def	130034..130516 (+)	483	WP_009328532.1	peptide deformylase	-
B34_RS00645 (B34_00133)	fmt	130521..131471 (+)	951	WP_069500462.1	methionyl-tRNA formyltransferase	-
B34_RS00650 (B34_00134)	rsmB	131461..132807 (+)	1347	WP_003181671.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
B34_RS00655 (B34_00135)	rlmN	132807..133892 (+)	1086	WP_003181673.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
B34_RS00660 (B34_00136)	-	133900..134664 (+)	765	WP_003181675.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 91315.68 Da
• Isoelectric Point: 9.0887

>NTDB_id=149840 B34_RS00635 WP_011197970.1 127587..130004(+) (priA) [Bacillus licheniformis strain SCDB 34]
MNFAEVIVDVTTKNIDRPFDYRIPDKFKGMIKKGMRVIVPFGARKIQGFVTSVKESSELAGRSVKDIEDILDLAPVLTEE
LLELSDWLTEKTLSFKITALQAMLPAAMKAKYGKEIKVIGDKPLPEPLKTLFSQKETLSYSDINDHGTLKLIQRQVQEGN
LEVAYKVKQKTNKKMRRMIAPLCAKEHLKEALAGLSPQAVKQKAILQFFIDRKEGGAVPAKDLIDKTGGSSASLKALIDK
GLLKESEEEVYRDPYQDRMFKKTEPLPLTEEQAQAYEAIADAIEEKRHDVFLLHGVTGSGKTEVYLQSIEKVLEAGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVLHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHETSYKQ
EEMPRYHAKEAAIQRAKYHRCPVVLGSATPTLESFARAKKGVYKLLSLKHRVNHQVMPDVSLVDMREELRSGNRSMFSKD
LMAGLEETLEKGEQAVLFLNKRGYSSFVMCRDCGYVIQCPHCEISLTYHRYGQRLKCHYCGYETGMPSSCPECGSEHIRY
FGTGTQRVEEELTKVLPAARVIRMDVDTTSRKGAHEKLLTSFGRGEADILLGTQMIAKGLDFENVTLVGVLSADTMLHIP
DFRAAEKTFQLMTQVSGRAGRHEKPGKVIIQTYTPSHYSIQLTKTHDYETFFRQEMLHRREQAYPPYYYLALVSVSHEEV
TKAALVTEKIATYLKKNISSEAKILGPVASPIARIKDRYRYQCVIKYKQENQLTSLLKNIMDHYKRDIEQKQLAISIDMN
PYMMM

Nucleotide

• Length: 2418 bp

>NTDB_id=149840 B34_RS00635 WP_011197970.1 127587..130004(+) (priA) [Bacillus licheniformis strain SCDB 34]
ATGAACTTCGCTGAAGTGATCGTCGATGTTACAACGAAAAATATCGACAGACCGTTTGATTACCGCATCCCCGACAAATT
CAAAGGTATGATTAAAAAAGGGATGCGCGTGATCGTTCCGTTCGGCGCCCGCAAAATCCAGGGATTTGTCACCAGCGTGA
AAGAATCGTCGGAGCTTGCCGGCCGTTCGGTTAAGGATATCGAGGATATCCTTGATCTGGCGCCCGTTTTGACGGAGGAG
CTGCTTGAGCTGTCAGACTGGCTGACAGAAAAAACACTTTCTTTTAAAATTACGGCTTTGCAGGCGATGCTCCCCGCGGC
TATGAAAGCAAAATACGGCAAAGAAATCAAGGTGATCGGCGATAAACCGCTTCCCGAACCGCTAAAAACCTTGTTCTCGC
AAAAAGAGACGCTTTCTTATTCCGATATAAATGACCACGGAACATTAAAGCTGATTCAGCGCCAGGTTCAAGAAGGCAAT
CTTGAAGTGGCGTATAAAGTAAAGCAGAAAACAAACAAAAAAATGCGGAGAATGATCGCCCCCCTTTGCGCGAAAGAACA
TTTAAAAGAAGCGCTTGCCGGCCTCTCTCCGCAAGCTGTCAAACAAAAAGCGATCCTTCAGTTTTTCATCGACCGCAAAG
AGGGCGGCGCTGTTCCGGCAAAGGATCTGATCGACAAAACGGGGGGCTCTTCCGCGTCCTTGAAAGCGCTCATCGACAAG
GGGCTGTTAAAGGAAAGCGAAGAGGAGGTTTACAGGGATCCTTATCAGGACAGAATGTTCAAAAAAACCGAACCGCTTCC
GCTGACTGAAGAGCAGGCGCAGGCCTACGAGGCGATAGCGGATGCAATCGAAGAAAAGCGGCACGATGTGTTTCTTCTTC
ACGGAGTGACCGGAAGCGGAAAAACGGAAGTCTATCTGCAATCGATCGAAAAGGTGCTGGAAGCCGGCAAAGAAGCGATC
GTTCTCGTTCCGGAAATTTCCCTGACGCCGCAGATGGTCAACCGCTTTAAAGGGCGCTTTGGCTCGCAGGTAGCCGTACT
GCACAGCGGGCTTTCAACAGGAGAAAAATATGATGAATGGCGGAAAATCCACCGCAAAGAAGTCCGGCTTGTCGTCGGGG
CGCGCTCGGCGATTTTCGCGCCGTTTGAAAATCTCGGCATGATTATAATTGATGAAGAGCACGAAACATCGTACAAGCAG
GAGGAGATGCCGAGATACCATGCGAAAGAAGCGGCGATTCAAAGGGCCAAATACCACCGGTGTCCGGTCGTGCTCGGCAG
TGCGACGCCGACGCTCGAATCGTTCGCGAGGGCGAAAAAAGGCGTGTACAAGCTTCTTTCTCTCAAGCACCGCGTCAATC
ACCAAGTCATGCCCGACGTGTCTCTCGTCGATATGAGAGAGGAACTGCGCAGCGGAAACCGCTCGATGTTTTCCAAAGAC
TTAATGGCCGGGCTTGAGGAAACTCTTGAAAAAGGAGAGCAGGCCGTTCTGTTTTTAAACAAAAGAGGCTATTCCTCATT
TGTCATGTGCAGGGACTGCGGATATGTCATCCAGTGTCCTCATTGTGAAATTTCATTGACGTACCACAGGTATGGACAGC
GTCTGAAGTGCCATTACTGCGGCTATGAAACCGGCATGCCGTCAAGCTGCCCCGAATGCGGAAGCGAGCATATCCGCTAC
TTTGGTACAGGCACACAGCGGGTCGAAGAAGAATTGACGAAGGTTCTGCCGGCTGCAAGGGTGATCCGCATGGATGTCGA
TACAACCTCAAGAAAGGGAGCGCATGAAAAGCTGCTCACATCCTTTGGCAGAGGCGAAGCCGATATCCTGCTTGGAACGC
AGATGATCGCCAAAGGGCTCGATTTCGAAAACGTCACGCTCGTCGGTGTCTTGAGCGCGGATACGATGCTTCACATCCCC
GATTTCAGAGCCGCTGAAAAAACGTTCCAGCTGATGACGCAGGTGAGCGGAAGAGCGGGGCGCCACGAAAAGCCGGGCAA
GGTGATCATTCAGACGTACACACCGTCCCATTACAGCATTCAGCTCACAAAAACGCATGATTATGAAACATTCTTCCGGC
AGGAGATGCTGCACAGAAGGGAACAGGCCTATCCTCCATATTATTATTTGGCGCTGGTCAGCGTATCGCACGAGGAAGTG
ACGAAGGCGGCACTCGTGACTGAAAAAATCGCCACCTATTTAAAAAAGAACATTTCAAGCGAGGCGAAGATTCTCGGCCC
TGTCGCATCTCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAAACCAGCTTA
CATCGTTGCTTAAAAACATTATGGATCATTACAAACGAGACATTGAACAAAAGCAATTGGCGATTTCGATCGACATGAAT
CCATATATGATGATGTAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Helicase conserved C-terminal domain (Helicase_C) (578-662)

• Type III restriction enzyme, res subunit (ResIII) (267-431)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (519-543)

• Primosomal protein N C-terminal domain (PriA_C) (706-801)

3D structure

• AlphaFold DB: Q65JS7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	79.006	100	0.79