Detailed information    

experimental Experimentally validated

Overview

Name   ruvC   Type   Machinery gene
Locus tag   ACIAD_RS09385 Genome accession   NC_005966
Coordinates   2029258..2029803 (+) Length   181 a.a.
NCBI ID   WP_026056842.1    Uniprot ID   Q6FAQ2
Organism   Acinetobacter baylyi ADP1     
Function   homologous recombination   
Homologous recombination

Function

HFIR was not decreased in a ruvC mutant of A. baylyi as could be expected from the above consideration, but significantly increased.


Genomic Context

Location: 2024258..2034803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS09370 (ACIAD2037) metK 2026565..2027731 (+) 1167 WP_004927441.1 methionine adenosyltransferase -
  ACIAD_RS09375 (ACIAD2038) - 2027805..2028056 (-) 252 WP_004927444.1 hypothetical protein -
  ACIAD_RS16995 (ACIAD2039) - 2028291..2028467 (+) 177 WP_004927447.1 hypothetical protein -
  ACIAD_RS09380 (ACIAD2040) - 2028566..2029132 (-) 567 WP_004927452.1 FxsA family protein -
  ACIAD_RS09385 (ACIAD2041) ruvC 2029258..2029803 (+) 546 WP_026056842.1 crossover junction endodeoxyribonuclease RuvC Machinery gene
  ACIAD_RS09390 (ACIAD2042) - 2029809..2031281 (-) 1473 WP_004927457.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
  ACIAD_RS09395 (ACIAD2043) queG 2031309..2032412 (+) 1104 WP_004927460.1 tRNA epoxyqueuosine(34) reductase QueG -
  ACIAD_RS09400 (ACIAD2045) bioB 2032503..2033492 (+) 990 WP_004927463.1 biotin synthase BioB -
  ACIAD_RS09405 (ACIAD2046) - 2033535..2033747 (-) 213 WP_004927467.1 hypothetical protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 19476.53 Da        Isoelectric Point: 10.3305

>NTDB_id=1495 ACIAD_RS09385 WP_026056842.1 2029258..2029803(+) (ruvC) [Acinetobacter baylyi ADP1]
MPLIIGIDPGSRLTGYGIIEKNGNELRFIDAGTIRTESQDMPERLKRIFAGVERIVKFHGPTQAAVEQVFMAQNPDSALK
LGQARGAAIAALVNLDLEVAEYTARQIKQSVVGYGAADKEQVQMMVMRILNLTIKPQSDAADALAAAICHAHASGSMSKM
AVLNALGGMARGRSRSSTRRR

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1495 ACIAD_RS09385 WP_026056842.1 2029258..2029803(+) (ruvC) [Acinetobacter baylyi ADP1]
ATGCCATTAATTATTGGAATTGATCCAGGTTCACGTTTAACAGGTTATGGAATCATTGAAAAAAATGGTAATGAGCTGCG
TTTTATCGATGCAGGCACAATTCGTACTGAAAGTCAGGACATGCCAGAACGATTAAAACGAATTTTTGCTGGTGTGGAAC
GAATTGTTAAATTTCATGGCCCAACACAAGCCGCGGTGGAACAAGTCTTTATGGCACAAAACCCCGACTCTGCACTTAAG
CTAGGCCAAGCCAGAGGTGCTGCAATTGCAGCTTTGGTCAATCTGGATCTGGAAGTAGCCGAATATACAGCAAGACAAAT
TAAACAATCGGTTGTGGGTTATGGTGCAGCCGACAAAGAACAGGTGCAGATGATGGTAATGCGTATATTAAATTTAACGA
TTAAACCACAATCCGATGCCGCAGATGCACTTGCAGCCGCCATTTGTCATGCACACGCTTCTGGCAGTATGAGTAAAATG
GCTGTTTTAAATGCTTTAGGTGGTATGGCACGTGGACGAAGCCGATCCAGTACCCGTCGTCGCTAA

Domains

Predicted by InterProScan.

Crossover junction endodeoxyribonuclease RuvC (RuvC)

(4-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6FAQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Nils Hülter et al. (2008) Double illegitimate recombination events integrate DNA segments through two different mechanisms during natural transformation of Acinetobacter baylyi. Molecular Microbiology 67(5):984-95. [PMID: 18194157]