Detailed information    

experimental Experimentally validated

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACIAD_RS15825 Genome accession   NC_005966
Coordinates   3424072..3425778 (-) Length   568 a.a.
NCBI ID   WP_004923374.1    Uniprot ID   Q6F719
Organism   Acinetobacter baylyi ADP1     
Function   homologous recombination   
Homologous recombination

Function

Deletion of recJ increased homology-facilitated illegitimate recombination (HFIR) frequency about 20-fold compared with wild type while homologous recombination was not affected. RecJ overproduction reduced the HFIR frequency to half compared with wild type.


Genomic Context

Location: 3419072..3430778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS15800 (ACIAD3493) - 3419180..3419599 (-) 420 WP_004923387.1 RidA family protein -
  ACIAD_RS15805 (ACIAD3496) - 3420162..3421232 (-) 1071 WP_004923383.1 alkene reductase -
  ACIAD_RS15810 (ACIAD3497) - 3421254..3421553 (-) 300 WP_004923380.1 ArsR/SmtB family transcription factor -
  ACIAD_RS15820 (ACIAD3499) - 3423251..3424024 (+) 774 WP_004923376.1 putative porin -
  ACIAD_RS15825 (ACIAD3500) recJ 3424072..3425778 (-) 1707 WP_004923374.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACIAD_RS15830 (ACIAD3501) pdxH 3425779..3426435 (-) 657 WP_004923370.1 pyridoxamine 5'-phosphate oxidase -
  ACIAD_RS15835 (ACIAD3502) glmM 3426451..3427782 (-) 1332 WP_004923367.1 phosphoglucosamine mutase -
  ACIAD_RS15840 (ACIAD3503) guaB 3427903..3429369 (-) 1467 WP_004923364.1 IMP dehydrogenase -
  ACIAD_RS15845 (ACIAD3504) - 3429497..3430513 (-) 1017 WP_004923359.1 AI-2E family transporter -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63267.56 Da        Isoelectric Point: 6.1177

>NTDB_id=1492 ACIAD_RS15825 WP_004923374.1 3424072..3425778(-) (recJ) [Acinetobacter baylyi ADP1]
MRTIQIKQRPLLTHPDQFQDVPEFIAHILARRGVDSIQQLDLKLKYLLKPTLKGLDTAIALIDQAIDQQQQIIIVGDYDA
DGATSTALMCLALKEMGARVDYIVPDRFKYGYGLTPAIADLAHQQCQPELLITVDNGISSHAGVEQAQQLGMKVIITDHH
LTTKSAPDAEAVVNPNQLNCPFPSKALAGVGVAFYLLAHLSSLRQAHHKPSCKITQYLDLVALGTYADVASLDYNNRILV
DAGLKRIQQQQCRAGILALLDIAKRDPSTLKAQDLGFVLGPRINAAGRMDSMNIGIECLLASDLETAYPIAEQLNRLNLE
RRQVETEMKQQALGYLEDIQLQHENIPPALVMFAENWHQGVIGIVAGRLKEQFHRPSIVFAPDTDGEHIKGSARSIEGIH
IRDTIEQVAEQHPHLVSHFGGHAAAAGLTLKKSHFEEFKNVFTACIAQMDETLLQAVLWTDGSLPAQAIDLETVDWIERL
GPWGQKFPLPQFEGRFEVLDYRWLKDIHLKLKLGLGEYGSVDAIAFNAAQKFDFDPMKKHVNLVYELDRNEFNGSVNLQL
RILHLDQT

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1492 ACIAD_RS15825 WP_004923374.1 3424072..3425778(-) (recJ) [Acinetobacter baylyi ADP1]
ATGCGCACAATTCAAATCAAACAGCGACCGTTACTCACACATCCTGATCAATTTCAGGATGTGCCCGAATTTATTGCGCA
TATTTTAGCCCGTCGAGGTGTCGACTCTATTCAGCAGTTAGATTTAAAGCTGAAATATTTGCTCAAGCCAACATTAAAGG
GCTTGGATACTGCAATTGCGCTGATTGATCAAGCCATCGACCAGCAACAGCAGATTATTATTGTGGGTGACTATGATGCA
GATGGTGCAACCAGTACGGCTTTGATGTGTCTAGCTTTAAAAGAAATGGGCGCAAGAGTTGATTACATTGTGCCTGATCG
CTTTAAGTATGGTTATGGCTTAACCCCTGCAATTGCTGATCTGGCGCATCAACAATGTCAACCAGAGCTGTTGATCACTG
TCGATAATGGTATTTCAAGTCATGCTGGTGTAGAGCAAGCACAGCAACTGGGCATGAAAGTGATTATTACCGATCATCAC
TTAACGACCAAATCTGCACCAGATGCTGAAGCCGTGGTTAATCCGAATCAACTTAATTGTCCGTTTCCAAGTAAAGCATT
GGCAGGGGTCGGGGTTGCATTTTATCTGTTGGCACATTTATCGAGCTTAAGGCAGGCACATCACAAACCAAGTTGCAAAA
TCACGCAATATCTGGATTTGGTCGCATTGGGTACATATGCCGATGTCGCGAGCTTAGATTATAACAATCGGATTTTGGTC
GATGCAGGTTTAAAACGGATTCAGCAACAGCAATGCCGTGCTGGCATACTGGCATTACTGGATATCGCTAAACGTGATCC
TTCGACGTTAAAAGCACAAGATTTAGGATTTGTACTTGGACCACGCATTAATGCAGCAGGTCGGATGGACAGCATGAATA
TCGGCATTGAATGCCTGCTTGCAAGTGATCTGGAAACTGCCTATCCGATTGCAGAGCAACTGAATCGACTTAATCTTGAG
CGTCGTCAGGTCGAAACGGAGATGAAACAGCAGGCACTTGGCTATCTTGAAGATATTCAGTTACAGCATGAAAATATTCC
GCCTGCCTTAGTCATGTTTGCAGAAAACTGGCATCAAGGTGTGATTGGAATTGTGGCGGGACGTCTTAAAGAGCAGTTTC
ATCGCCCCAGCATTGTATTTGCACCTGATACGGACGGGGAGCATATTAAAGGATCTGCCCGTTCTATCGAAGGTATTCAC
ATCCGCGATACGATTGAACAGGTTGCAGAACAACATCCTCATTTAGTCAGTCATTTTGGGGGGCATGCGGCGGCGGCGGG
CTTAACCCTGAAAAAAAGTCACTTTGAAGAATTTAAAAACGTATTCACAGCGTGTATTGCGCAAATGGATGAAACTTTGC
TGCAAGCGGTGCTCTGGACCGATGGCTCTTTGCCTGCACAGGCTATTGATCTGGAAACTGTCGACTGGATCGAGCGTCTA
GGGCCTTGGGGACAAAAGTTCCCACTTCCACAGTTTGAAGGACGTTTTGAAGTGCTGGATTATCGCTGGCTGAAAGATAT
ACATCTCAAGTTAAAACTTGGCTTGGGAGAATATGGCTCTGTAGATGCGATTGCATTTAATGCAGCACAAAAGTTTGATT
TTGATCCAATGAAGAAACATGTCAATTTGGTATATGAGCTAGATCGTAATGAATTTAATGGCAGTGTAAATTTACAACTT
CGCATCTTACATTTAGATCAGACATAA

Domains

Predicted by InterProScan.

DHH family (DHH)

(71-225)

DHHA1 domain (DHHA1)

(314-442)

RecJ OB domain (RecJ_OB)

(461-562)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6F719

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Nils Hülter et al. (2008) Double illegitimate recombination events integrate DNA segments through two different mechanisms during natural transformation of Acinetobacter baylyi. Molecular Microbiology 67(5):984-95. [PMID: 18194157]
[2] Klaus Harms et al. (2007) The RecJ DNase strongly suppresses genomic integration of short but not long foreign DNA fragments by homology-facilitated illegitimate recombination during transformation of Acinetobacter baylyi. Molecular Microbiology 64(3):691-702. [PMID: 17462017]