Detailed information NTG0149138

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: AS588_RS07485
• Genome accession: NZ_CP014700
• Coordinates: 1629612..1632023 (-)
• Length: 803 a.a.
• NCBI ID: WP_014304990.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain S499
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1624612..1637023

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS588_RS07460 (AS588_07460)	-	1624955..1625716 (-)	762	WP_015388247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
AS588_RS07465 (AS588_07465)	rlmN	1625722..1626813 (-)	1092	WP_003154339.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
AS588_RS07470 (AS588_07470)	rsmB	1626817..1628160 (-)	1344	WP_003154341.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
AS588_RS07475 (AS588_07475)	fmt	1628147..1629100 (-)	954	WP_003154342.1	methionyl-tRNA formyltransferase	-
AS588_RS07480 (AS588_07480)	def	1629105..1629587 (-)	483	WP_003154343.1	peptide deformylase	-
AS588_RS07485 (AS588_07485)	priA	1629612..1632023 (-)	2412	WP_014304990.1	primosomal protein N'	Machinery gene
AS588_RS07490 (AS588_07490)	coaBC	1632020..1633240 (-)	1221	WP_015388248.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
AS588_RS07495 (AS588_07495)	rpoZ	1633331..1633534 (-)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
AS588_RS07500 (AS588_07500)	gmk	1633538..1634152 (-)	615	WP_003154347.1	guanylate kinase	-
AS588_RS07505 (AS588_07505)	remA	1634160..1634429 (-)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
AS588_RS07510 (AS588_07510)	-	1634506..1635381 (-)	876	WP_003154356.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90849.83 Da
• Isoelectric Point: 8.7169

>NTDB_id=149138 AS588_RS07485 WP_014304990.1 1629612..1632023(-) (priA) [Bacillus amyloliquefaciens strain S499]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGSMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIGGGEKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKQAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=149138 AS588_RS07485 WP_014304990.1 1629612..1632023(-) (priA) [Bacillus amyloliquefaciens strain S499]
ATGAATGTAGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGATTCGTCCGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACGCCTGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCCGCCGC
ATTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAGAAATTCGTGCGTAAAGGAAGC
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAGCAGGCTTCTAAACAGCTCGCCGTGCTTACTTATTTTATCGGCGGGGGAG
AAAAGATTTCCGCGGCTGAACTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGTGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCAGGCCGCTTTTGAGCCGATTAAACAAGCTGTAGCCGACCATGTCCATCACGTCTTTCTGCTTCACGGCG
TCACGGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTCGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTTCCGTTAAAACATCGCGTAAACCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTCTCAAGAGAGCTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCCGTCCTTTTTCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGATTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCCATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGTGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAGATGA
TCGCGAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTGAGCGCAGATACGACGCTTCACATCCCAGATTTC
AGGGCCGCGGAGAAGACATTCCAGCTGCTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGACGGGCCCAGTCATACCCGCCGTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	84.348	100	0.846