Detailed information    

experimental Experimentally validated

Overview

Name   recN   Type   Machinery gene
Locus tag   C694_RS07175 Genome accession   NC_018939
Coordinates   1453395..1454969 (-) Length   524 a.a.
NCBI ID   WP_001204425.1    Uniprot ID   O25943
Organism   Helicobacter pylori 26695     
Function   homologous recombination   
Homologous recombination

Function

Inactivation of recA, recB, recN, ruvA, ruvB, and ruvC significantly reduced the number of recombinant clones.


Genomic Context

Location: 1448395..1459969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS07140 (C694_07160) - 1448749..1449610 (+) 862 Protein_1351 3'-5' exonuclease -
  C694_RS09065 - 1449632..1449775 (-) 144 WP_181372295.1 hypothetical protein -
  C694_RS07150 (C694_07170) - 1450207..1450656 (+) 450 WP_001862625.1 hypothetical protein -
  C694_RS07155 (C694_07175) - 1450658..1450981 (+) 324 WP_001862626.1 hypothetical protein -
  C694_RS07160 (C694_07180) - 1451023..1451523 (-) 501 WP_001294251.1 hypothetical protein -
  C694_RS07165 (C694_07185) - 1451776..1452072 (-) 297 WP_000539103.1 hypothetical protein -
  C694_RS07170 (C694_07190) - 1452091..1453398 (-) 1308 WP_000667832.1 NFACT family protein -
  C694_RS07175 (C694_07195) recN 1453395..1454969 (-) 1575 WP_001204425.1 DNA repair protein RecN Machinery gene
  C694_RS07180 (C694_07200) - 1454984..1455838 (-) 855 WP_000655300.1 NAD(+)/NADH kinase -
  C694_RS07185 (C694_07210) - 1456026..1456754 (+) 729 WP_000719289.1 outer membrane protein -
  C694_RS07190 - 1456765..1458486 (-) 1722 Protein_1361 DUF262 domain-containing protein -
  C694_RS07195 (C694_07225) - 1458601..1459743 (-) 1143 WP_000152122.1 alanine dehydrogenase -

Sequence

Protein


Download         Length: 524 a.a.        Molecular weight: 59564.66 Da        Isoelectric Point: 6.0724

>NTDB_id=1491 C694_RS07175 WP_001204425.1 1453395..1454969(-) (recN) [Helicobacter pylori 26695]
MRDFNNAQITRLKVRQNAVFEKLDLEFKDGLSAISGASGVGKSVLIASLLGAFGLKESNASNIEVELIAPFLDTEEYGIF
REDEHEPLVISVIKKEKTRYFLNQTSLSKNTLKALLKGLIKRLSNDRFSQNELNDILMLSLLDGYIQNENKAFSPLLGAL
EEKFTRLEKLEKERRLLEDKKRFQKDLEERLNFEKMKLERLDLKEDEYERLLEQKKLLSSKEKLNDKIALALEVLENTHK
ITHALESVGHSAEFLKSALLEASALLEKEQAKLEECERLDIEKVLERLGMLSGIIKDYGSIMHAKERLGHVKNELHNLKE
IDSHCETYHKEIERLKTECLKLCEEISGFRKEYLAGFNALLSAKAKDLLLKSPSLVLEDAPMSEKGAQKLVLNLQNSQLE
TLSSGEYSRLRLAFMLLEMEFLKDFKGVLVLDEMDSNLSGEESLAVSKALETLSSHSQIFAISHQVHIPALAKNHILVFK
ENHKSLAKTLNNEERVLEIARMIGGSENIESAISFAKEKLKAQE

Nucleotide


Download         Length: 1575 bp        

>NTDB_id=1491 C694_RS07175 WP_001204425.1 1453395..1454969(-) (recN) [Helicobacter pylori 26695]
ATGCGAGATTTCAATAACGCTCAAATCACACGCTTAAAAGTGCGTCAAAACGCTGTTTTTGAAAAATTGGATCTGGAGTT
TAAAGACGGCTTGAGCGCGATTAGTGGGGCTAGCGGGGTGGGAAAAAGCGTTCTTATTGCGAGTCTTTTAGGGGCGTTTG
GGCTTAAAGAGAGCAACGCTTCAAACATTGAAGTGGAATTGATTGCGCCTTTTTTAGACACTGAAGAATACGGCATTTTT
AGAGAAGATGAACATGAACCCTTAGTCATCAGCGTGATTAAAAAAGAAAAAACGCGCTATTTTTTAAACCAAACAAGCCT
GTCTAAAAACACGCTCAAAGCGTTATTAAAAGGTTTGATTAAACGCTTGTCTAACGATAGATTCAGCCAGAATGAACTCA
ACGATATTTTAATGCTCTCCTTACTGGATGGCTATATCCAAAACGAAAATAAGGCGTTTAGCCCCCTTTTAGGCGCGCTT
GAAGAAAAATTCACCCGATTAGAGAAGCTAGAAAAAGAAAGGCGATTGTTAGAGGATAAAAAGCGTTTCCAAAAGGATTT
AGAAGAACGATTGAATTTTGAAAAAATGAAATTAGAGAGGCTGGATTTAAAAGAAGATGAATACGAACGCCTTTTAGAGC
AAAAAAAATTGCTTTCTAGTAAGGAAAAACTGAACGATAAAATCGCTCTGGCGTTAGAGGTGCTAGAAAATACCCATAAA
ATCACGCATGCTTTAGAGAGCGTGGGCCATAGCGCGGAGTTTTTAAAAAGCGCTTTATTAGAAGCGAGCGCTCTATTGGA
AAAAGAGCAGGCTAAATTAGAAGAGTGCGAGCGTTTGGACATTGAAAAAGTTCTAGAAAGGCTTGGCATGCTAAGTGGGA
TCATTAAGGATTACGGGAGTATTATGCATGCTAAAGAACGATTAGGGCATGTTAAAAACGAATTGCACAACCTAAAAGAA
ATTGATAGTCATTGCGAAACTTACCACAAAGAAATAGAGCGATTAAAAACCGAATGCTTGAAATTGTGCGAAGAAATAAG
CGGCTTTAGAAAAGAGTATTTAGCCGGTTTTAACGCTCTTTTAAGCGCTAAAGCGAAAGATTTGCTCCTAAAAAGCCCCA
GTTTGGTTTTAGAAGACGCCCCCATGAGCGAAAAAGGCGCTCAAAAACTCGTTTTGAATTTGCAAAATTCCCAATTAGAA
ACCTTAAGCTCTGGAGAATACAGCCGTTTGAGACTGGCGTTCATGCTTTTAGAAATGGAATTTTTAAAGGATTTTAAAGG
CGTGTTGGTGTTAGATGAAATGGATTCCAATTTGAGCGGCGAAGAGAGTTTGGCGGTCTCTAAAGCCCTTGAAACCTTAA
GCAGCCATTCGCAAATCTTTGCCATTTCGCACCAAGTCCATATCCCAGCGCTCGCTAAAAACCATATTTTAGTCTTCAAA
GAAAACCACAAAAGCCTTGCCAAAACCCTTAATAACGAAGAAAGGGTTTTAGAAATCGCGCGCATGATAGGGGGGAGCGA
GAATATAGAGAGCGCGATTTCTTTCGCTAAGGAAAAATTAAAGGCGCAAGAATGA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(10-483)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O25943

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Stefan Kulick et al. (2008) Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PloS One 3(11):e3797. [PMID: 19030104]