Detailed information    

experimental Experimentally validated

Overview

Name   recB   Type   Machinery gene
Locus tag   C694_RS07990 Genome accession   NC_018939
Coordinates   1632623..1635460 (-) Length   945 a.a.
NCBI ID   WP_000380097.1    Uniprot ID   O26077
Organism   Helicobacter pylori 26695     
Function   homologous recombination   
Homologous recombination

Function

Inactivation of recA, recB, recN, ruvA, ruvB, and ruvC significantly reduced the number of recombinant clones.


Genomic Context

Location: 1627623..1640460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS07965 (C694_08020) - 1627950..1628288 (-) 339 WP_000383778.1 DUF6394 family protein -
  C694_RS07970 (C694_08025) secF 1628298..1629269 (-) 972 WP_000418663.1 protein translocase subunit SecF -
  C694_RS07975 (C694_08030) secD 1629278..1630855 (-) 1578 WP_000765381.1 protein translocase subunit SecD -
  C694_RS07980 (C694_08035) yajC 1630852..1631235 (-) 384 WP_000529779.1 preprotein translocase subunit YajC -
  C694_RS07985 (C694_08040) nhaA 1631283..1632599 (-) 1317 WP_001049644.1 sodium/proton antiporter NhaA -
  C694_RS07990 (C694_08045) recB 1632623..1635460 (-) 2838 WP_000380097.1 RecB-like helicase Machinery gene
  C694_RS07995 (C694_08050) rpsB 1635709..1636503 (+) 795 WP_000258283.1 30S ribosomal protein S2 -
  C694_RS08000 (C694_08055) tsf 1636503..1637570 (+) 1068 WP_000014397.1 translation elongation factor Ts -
  C694_RS08005 (C694_08060) - 1638019..1639867 (-) 1849 Protein_1522 peptidoglycan D,D-transpeptidase FtsI family protein -
  C694_RS08550 - 1639874..1639999 (-) 126 WP_080012135.1 transcriptional regulator -
  C694_RS08010 (C694_08070) fliE 1640023..1640352 (-) 330 WP_001147918.1 flagellar hook-basal body complex protein FliE -

Sequence

Protein


Download         Length: 945 a.a.        Molecular weight: 108880.80 Da        Isoelectric Point: 5.6195

>NTDB_id=1487 C694_RS07990 WP_000380097.1 1632623..1635460(-) (recB) [Helicobacter pylori 26695]
MDTKRQCMALKASAGSGKTFALSVRFLALLFKGANPSEILTLTFTKKATAEMKERILDYLKILQQENLENEKEKSQNILK
ELEEKYHLDPSLVQNSAPKIYQRFLNAEIRISTIDAFFQSILRKFCWFVGLSANFEVNEDTKAHQQQLNEGFLSALNGEQ
LEALSVFIAQCLSYDSYTSDSILERLRFLKNKLYLFDPNKKEPAFDEKDFLEKLRSLNEQIQSIETASDRAKTAIKCDDF
RGFLNSSLTWLEKKSEYQSFKKLKSEIPTLESECEEIENDLKRYYEAKETAIFKKFPKFIQLYDNATSKIQALDFDAIKD
KVHVLLNGYEEMPAEFFYFRLDSKIAHILIDEFQDTSLNDYKILAPFIDEIKAGIGQAKWHRSVFFVGDVKQSIYAFRGS
FSSLFESVSKDFYHDNLEFNHRSAPLIINYVNTIFKKAYQNSPTAYLEQKYPKTSQNKHVTDGYVKVSLVADERELLLDQ
VLQEAQNLLEHRIEPKDITILCATNDDALEIKNYLQERLSAIRPSTESSAKLSQFVESKIIKNALEYALAEEPYKPFYKH
SVLKLAGYLHDDAIALAGFNPKKESVAGFVWKVMEQFELYGEPAQSCLELAVGCEDADGFLEKLETKAIASSHSKGAQIM
TIHKSKGMQFPYVIVCERLGKPNSSHSNQLLEEYDGTELLRLYYRMKNREVVDKDYARALDKEEAAKDHEEINVYYVAFT
RAELGLIVVAKDKKESKKESKNKTMREKLDLVPLEEGEIAPVISPQKEPLITSTLIKPHAYGEQVQEIEEEPSDYEKNND
QEAINFGIALHKGLEYQYAYNIPKQSVLEYLNYHHGFYGLDYQALEESLELFENDAEIQALFKNLPLKGEAAFLFQGVVS
RIDVLLWDRGQNLYVLDYKSSQNYQQSHKAQVSHYAEFLKTQAPHFKIQAGIIYAHKRLLEKLWV

Nucleotide


Download         Length: 2838 bp        

>NTDB_id=1487 C694_RS07990 WP_000380097.1 1632623..1635460(-) (recB) [Helicobacter pylori 26695]
ATGGATACCAAAAGACAATGCATGGCTTTAAAGGCTTCAGCAGGGAGCGGGAAGACTTTCGCTTTGAGCGTGCGGTTTTT
AGCCCTATTGTTTAAGGGGGCTAATCCTAGCGAGATTTTGACGCTCACTTTCACTAAAAAAGCCACCGCCGAAATGAAAG
AGCGCATTTTAGACTATTTAAAAATTTTGCAACAAGAAAACCTTGAAAATGAAAAAGAAAAATCCCAAAATATCCTAAAA
GAATTAGAAGAAAAATACCATTTAGACCCTAGCTTGGTGCAAAATAGCGCTCCAAAAATCTACCAACGCTTTTTAAACGC
TGAAATTAGAATCAGCACTATTGATGCGTTTTTCCAAAGCATTTTAAGGAAATTTTGCTGGTTTGTGGGGTTGAGCGCGA
ATTTTGAAGTCAATGAAGACACCAAAGCGCACCAGCAACAGCTTAATGAGGGTTTTTTGAGCGCTTTAAATGGCGAACAG
CTTGAAGCATTGAGCGTTTTTATCGCTCAATGCTTAAGTTATGATAGTTACACAAGCGATTCTATTTTAGAGCGGTTGCG
TTTTTTAAAAAACAAGCTCTATTTATTTGATCCTAATAAAAAAGAGCCTGCATTTGATGAAAAGGATTTTTTAGAAAAAC
TAAGGAGCTTAAACGAACAAATTCAAAGCATAGAAACAGCGTCAGATAGGGCTAAAACAGCCATTAAATGCGATGATTTT
AGGGGATTTTTAAACAGCTCTTTAACCTGGCTTGAAAAAAAGAGCGAATATCAATCTTTCAAAAAACTTAAAAGTGAAAT
CCCCACTTTAGAGAGCGAATGCGAAGAGATTGAAAACGATTTAAAACGCTATTATGAAGCCAAAGAAACCGCAATATTTA
AAAAATTCCCTAAATTCATCCAACTTTATGATAACGCCACTTCTAAAATCCAAGCCTTAGATTTTGATGCGATTAAAGAT
AAAGTCCATGTCTTATTGAATGGTTATGAAGAAATGCCGGCGGAGTTTTTTTATTTCAGGTTGGACAGCAAGATCGCGCA
CATTTTGATTGATGAGTTTCAAGACACGAGCTTGAACGATTATAAGATTTTAGCCCCTTTTATTGATGAGATTAAAGCCG
GGATAGGGCAAGCGAAATGGCACAGGAGCGTGTTTTTTGTGGGCGATGTCAAGCAGAGCATTTATGCCTTTAGGGGGAGT
TTTAGCTCTTTGTTTGAAAGCGTTTCTAAGGATTTTTACCACGATAATTTGGAATTTAACCACCGCAGTGCGCCTTTAAT
CATTAATTATGTGAACACCATTTTTAAAAAAGCTTATCAAAATTCCCCCACCGCTTATTTGGAGCAAAAATACCCTAAAA
CTTCTCAAAATAAACATGTTACAGACGGCTATGTTAAAGTCTCTTTAGTGGCTGATGAAAGAGAATTGTTATTAGATCAG
GTCTTACAAGAAGCTCAAAACCTTTTAGAACATCGTATTGAGCCTAAAGATATTACCATTTTATGCGCCACTAATGACGA
CGCTTTAGAAATCAAAAATTATTTGCAAGAGCGTTTGAGTGCGATTCGCCCAAGCACGGAATCTAGCGCAAAATTGTCTC
AATTTGTAGAGTCTAAAATCATTAAGAACGCTTTAGAATACGCTCTAGCGGAAGAACCTTACAAGCCTTTTTATAAGCAC
AGCGTTTTAAAACTCGCTGGATACTTGCATGATGATGCGATCGCTTTAGCTGGTTTTAACCCTAAAAAAGAGAGCGTGGC
AGGCTTTGTGTGGAAGGTGATGGAGCAATTTGAGCTTTATGGAGAGCCTGCACAAAGCTGTTTGGAATTAGCGGTTGGGT
GCGAAGATGCCGATGGATTTTTAGAAAAATTAGAGACTAAAGCGATCGCTTCTTCCCATTCAAAAGGCGCGCAGATCATG
ACCATTCACAAATCTAAAGGCATGCAATTCCCTTATGTGATCGTGTGCGAACGCTTGGGCAAGCCTAATTCAAGCCATTC
CAATCAACTCCTTGAAGAATATGACGGCACAGAGCTTCTTCGCCTTTATTACAGAATGAAAAATCGTGAGGTCGTAGATA
AAGATTACGCTAGGGCTTTAGACAAAGAAGAAGCGGCCAAAGATCATGAAGAGATTAATGTGTATTATGTCGCATTCACT
AGGGCTGAGTTAGGGCTGATTGTCGTGGCCAAAGACAAAAAAGAAAGCAAAAAAGAAAGCAAAAACAAAACAATGCGCGA
AAAATTGGATCTTGTGCCTTTAGAAGAGGGAGAAATCGCGCCGGTTATTTCTCCACAAAAAGAGCCTTTAATTACAAGCA
CTCTCATCAAGCCCCATGCCTATGGCGAGCAAGTCCAAGAGATAGAAGAAGAGCCTAGCGATTATGAAAAGAATAACGAC
CAGGAAGCGATCAACTTTGGTATCGCTTTGCACAAGGGGTTAGAATACCAATACGCTTATAACATTCCTAAACAAAGCGT
TTTAGAATATTTAAACTACCATCATGGTTTTTATGGTTTGGATTACCAGGCGTTAGAAGAAAGTTTAGAGCTTTTTGAAA
ACGATGCAGAGATACAAGCTCTTTTTAAAAATTTGCCCTTAAAGGGCGAAGCGGCTTTTTTATTCCAAGGGGTTGTGTCT
AGGATAGATGTTTTGTTGTGGGATAGGGGGCAAAATTTGTATGTTTTAGATTACAAAAGCTCTCAAAATTACCAGCAAAG
CCATAAAGCGCAAGTGTCTCATTATGCTGAGTTTTTGAAAACTCAAGCCCCCCATTTTAAGATACAAGCGGGCATTATTT
ACGCTCATAAAAGACTGCTTGAAAAATTATGGGTCTGA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(633-729)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(825-937)

UvrD-like helicase C-terminal domain (UvrD_C)

(417-518)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(8-412)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O26077

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Stefan Kulick et al. (2008) Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PloS One 3(11):e3797. [PMID: 19030104]