Detailed information    

experimental Experimentally validated

Overview

Name   comF   Type   Machinery gene
Locus tag   SGL_RS13010 Genome accession   NC_000911
Coordinates   2392729..2393403 (+) Length   224 a.a.
NCBI ID   WP_010873338.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

When a positively phototactic strain of this cyanobacterium was insertionally inactivated at slr0388, the mutants were not transformable, and appeared to aggregate as a result of increased bundling of type IV pili.


Genomic Context

Location: 2387729..2398403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS19465 - 2388867..2389031 (+) 165 WP_158299078.1 hypothetical protein -
  SGL_RS12990 (sll0328) - 2389062..2389481 (-) 420 WP_010873334.1 hypothetical protein -
  SGL_RS12995 (sll0327) - 2389481..2389900 (-) 420 WP_010873335.1 hypothetical protein -
  SGL_RS13000 (slr0386) - 2390249..2391454 (+) 1206 WP_041425833.1 SGNH/GDSL hydrolase family protein -
  SGL_RS13005 (slr0387) - 2391547..2392707 (+) 1161 WP_010873337.1 cysteine desulfurase family protein -
  SGL_RS13010 (slr0388) comF 2392729..2393403 (+) 675 WP_010873338.1 ComF family protein Machinery gene
  SGL_RS13015 (slr0769) - 2393493..2394107 (+) 615 WP_010873339.1 L,D-transpeptidase -
  SGL_RS13020 (slr0770) - 2394232..2394552 (+) 321 WP_010873340.1 DUF433 domain-containing protein -
  SGL_RS13025 (slr0771) - 2394536..2394871 (+) 336 WP_010873341.1 DUF5615 family PIN-like protein -
  SGL_RS13030 (slr0772) bchB 2395103..2396629 (+) 1527 WP_010873342.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) subunit B -
  SGL_RS13035 (slr0773) - 2396761..2397441 (+) 681 WP_010873343.1 potassium channel family protein -

Sequence

Protein


Download         Length: 224 a.a.        Molecular weight: 25620.62 Da        Isoelectric Point: 10.8654

>NTDB_id=1422 SGL_RS13010 WP_010873338.1 2392729..2393403(+) (comF) [Synechocystis sp. PCC 6803]
MWQSIRSLIFQTPCPLCQKPSQDQLCRDCTGQILSHRFPRKFQQWQGNFPRFVWGQYEGQLRRALTVMKFEQQPDIGIWL
GEQLAEQWLQQPQAKTKLRPQVVPIPLNARKQAQRGFNQAERIAAGFCRLTGYVLHPQALQRVKDTQALFGLSPGDRRRE
LQSALATGPGFNTRRPWLILDDIITTGTTALEARRIMEEKGAKVLGIVAIAAPSFSQPMELRQQ

Nucleotide


Download         Length: 675 bp        

>NTDB_id=1422 SGL_RS13010 WP_010873338.1 2392729..2393403(+) (comF) [Synechocystis sp. PCC 6803]
ATGTGGCAATCAATTCGTTCCCTCATTTTTCAAACTCCCTGTCCTCTATGCCAAAAACCCAGTCAGGATCAACTTTGTCG
GGATTGCACTGGACAAATTTTGAGCCATCGTTTTCCTCGTAAATTCCAGCAATGGCAAGGAAACTTTCCCCGCTTTGTTT
GGGGACAATACGAAGGACAATTGCGGCGGGCTTTAACGGTGATGAAGTTTGAGCAACAGCCAGACATTGGCATTTGGTTG
GGGGAACAGTTGGCAGAACAGTGGTTACAACAACCCCAAGCTAAAACTAAATTGCGTCCCCAGGTGGTGCCCATTCCCCT
AAACGCTCGTAAGCAAGCCCAACGGGGATTTAATCAAGCTGAAAGAATTGCCGCTGGTTTTTGCCGCCTGACTGGTTATG
TTCTCCACCCCCAAGCATTACAACGAGTTAAAGATACCCAAGCTCTGTTTGGTCTTAGCCCAGGCGATCGCCGCCGGGAA
TTGCAATCAGCTTTAGCCACGGGGCCAGGGTTTAACACTCGCCGACCCTGGTTGATTTTGGATGATATTATTACGACCGG
CACCACCGCCCTAGAAGCCCGACGGATAATGGAAGAAAAGGGAGCTAAAGTGTTGGGTATCGTGGCGATCGCCGCACCGT
CTTTTTCCCAGCCCATGGAACTTAGACAGCAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Kenlee Nakasugi et al. (2006) The competence gene, comF, from Synechocystis sp. strain PCC 6803 is involved in natural transformation, phototactic motility and piliation. Microbiology (Reading, England) 152(Pt 12):3623-3631. [PMID: 17159215]