Detailed information    

experimental Experimentally validated

Overview

Name   pilA/pilA5   Type   Machinery gene
Locus tag   SGL_RS12295 Genome accession   NC_000911
Coordinates   2221173..2221631 (+) Length   152 a.a.
NCBI ID   WP_020862233.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

In this study, we show that the minor pilin PilA5 is essential for natural transformation but is dispensable for motility and flocculation.


Genomic Context

Location: 2216173..2226631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS12280 (sll1864) - 2216476..2219175 (-) 2700 WP_010873202.1 chloride channel protein -
  SGL_RS12285 (slr1926) - 2219290..2219793 (+) 504 WP_020862235.1 hypothetical protein -
  SGL_RS12290 (slr1927) - 2219741..2220961 (+) 1221 WP_010873204.1 DUF58 domain-containing protein -
  SGL_RS12295 (slr1928) pilA/pilA5 2221173..2221631 (+) 459 WP_020862233.1 hypothetical protein Machinery gene
  SGL_RS18960 - 2221648..2222199 (+) 552 WP_010873206.1 pilus assembly FimT family protein -
  SGL_RS12305 (slr1930) - 2222316..2222876 (+) 561 WP_010873207.1 pilus assembly FimT family protein -
  SGL_RS18965 - 2222898..2223815 (+) 918 WP_010873208.1 PilW family protein -
  SGL_RS12310 (slr1932) - 2223873..2224568 (+) 696 WP_010873209.1 alpha/beta fold hydrolase -
  SGL_RS12315 (sll1863) - 2224789..2225079 (-) 291 WP_223211274.1 hypothetical protein -
  SGL_RS12320 (sll1862) - 2225278..2225709 (-) 432 WP_010873211.1 hypothetical protein -
  SGL_RS12325 (sll1861) - 2225766..2226530 (-) 765 WP_010873212.1 IS5 family transposase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16141.38 Da        Isoelectric Point: 4.3321

>NTDB_id=1420 SGL_RS12295 WP_020862233.1 2221173..2221631(+) (pilA/pilA5) [Synechocystis sp. PCC 6803]
MFEVLIALMISFLFLTGTLNAMVMATVMRVKAEREAQAGYWIKEDLEQVRALAANFNPATGNGCPTSVGETFRDANNSAD
ADPNNDGLASLPVPANKSIVGVTAAITRTPGVSNNVLTLSYRVRNTNVTDNDMATLATLYTEVLPAAALACE

Nucleotide


Download         Length: 459 bp        

>NTDB_id=1420 SGL_RS12295 WP_020862233.1 2221173..2221631(+) (pilA/pilA5) [Synechocystis sp. PCC 6803]
ATGTTCGAGGTGCTGATTGCCTTGATGATTTCCTTTCTATTTCTGACAGGAACCCTGAATGCCATGGTAATGGCAACGGT
AATGCGGGTTAAAGCGGAAAGAGAGGCCCAAGCTGGCTATTGGATAAAAGAAGATTTAGAGCAAGTTCGGGCATTAGCTG
CAAACTTCAATCCGGCAACTGGTAATGGATGCCCTACAAGTGTGGGTGAAACTTTTCGAGATGCAAACAATAGTGCTGAC
GCCGATCCTAACAATGATGGACTAGCGAGCTTACCAGTTCCTGCCAACAAATCAATTGTCGGTGTAACAGCCGCTATTAC
TAGAACACCAGGGGTATCTAATAACGTTTTAACTCTTAGCTATCGTGTCAGGAACACCAATGTCACCGACAATGATATGG
CTACCCTTGCAACACTTTACACCGAGGTTCTTCCTGCTGCCGCTCTTGCTTGTGAATAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Sabrina Oeser et al. (2021) Minor pilins are involved in motility and natural competence in the cyanobacterium Synechocystis sp. PCC 6803. Molecular Microbiology 116(3):743-765. [PMID: 34115422]