Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BIS30_RS03205 Genome accession   NZ_CP011051
Coordinates   560200..562560 (-) Length   786 a.a.
NCBI ID   WP_003222692.1    Uniprot ID   -
Organism   Bacillus spizizenii strain T30     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 555200..567560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIS30_RS03185 (BIS30_03185) - 555278..556831 (+) 1554 WP_003222700.1 SH3 domain-containing protein -
  BIS30_RS03190 (BIS30_03190) dtd 556856..557296 (-) 441 WP_003222698.1 D-aminoacyl-tRNA deacylase -
  BIS30_RS03195 (BIS30_03195) relA 557309..559513 (-) 2205 WP_003222696.1 GTP diphosphokinase -
  BIS30_RS03200 (BIS30_03200) - 559682..560194 (-) 513 WP_003222694.1 adenine phosphoribosyltransferase -
  BIS30_RS03205 (BIS30_03205) recJ 560200..562560 (-) 2361 WP_003222692.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BIS30_RS03210 (BIS30_03210) - 562627..562959 (-) 333 WP_003222691.1 lipopolysaccharide assembly LapA domain-containing protein -
  BIS30_RS03215 (BIS30_03215) - 563036..563533 (-) 498 WP_003222688.1 cation:proton antiporter regulatory subunit -
  BIS30_RS03220 (BIS30_03220) secDF 563691..565904 (-) 2214 WP_003222686.1 protein translocase subunit SecDF -
  BIS30_RS03225 (BIS30_03225) comN 565943..566239 (-) 297 WP_003222684.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88163.70 Da        Isoelectric Point: 4.9707

>NTDB_id=141764 BIS30_RS03205 WP_003222692.1 560200..562560(-) (recJ) [Bacillus spizizenii strain T30]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAVDRIKQAISQQ
EQIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPNVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELVKLSGGDIGEANEETVGFQIAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKSGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLNAEDVPDLRNRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESITEMNLLSPFGMLN
PKPHVLVENAVLEDVRKIGANKNHVKMTVRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRMWEDTVSVLPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSEDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNQEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELARHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGVKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=141764 BIS30_RS03205 WP_003222692.1 560200..562560(-) (recJ) [Bacillus spizizenii strain T30]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTCGAAATGAAGGGCATGAAAGAAGCGGTTGATCGTATAAAACAAGCGATTTCACAACAA
GAACAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACCAGTACGTCAGTGATGCTTCACACACTGCAAAAGCT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGCTTTAAAGAAGGATATGGCCCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTCTGATTATTACAGTTGATACAGGGATTGCCGCTGTACATGAAGCGAAGGTGGCAAAGGAACTT
GGGCTGGATGTCATTATTACCGATCACCATGAGCCGGGACCGGAGCTTCCGAATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACCTACCCGTTTAAAGAACTTGCCGGAGTTGGGGTCGCGTTTAAGCTCGCCCACGCTTTATTGGGCGAAC
TCCCGGATGAACTGCTTGACTTGGCGGCAATCGGCACCATTGCCGATCTTGTCCCGTTGCATGACGAAAATAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGTCGGACGAATCGACTGGGCTTAAAAGAGCTGGTCAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACTGTCGGGTTCCAGATAGCACCAAGGCTGAATGCCGTCGGACGAATCGAACAAGCGGATC
CCGCTGTTCATTTGCTCATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCTGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGTAAAATGACGGATGAAGCGATTGAAATGGTTGAGCAGCAAGGTCTCGATCAAACTGCAATCGT
CGTGGCCAAATCCGGGTGGAATCCAGGAGTTGTGGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTACCGTCCGGCCA
TTGTGCTTGGCATTGATGAAGAAAAAGGGATCGCTAAAGGCTCGGCCCGAAGCATTAGAGGCTTTAACTTATTCGAGAGC
CTTTCAGAGTGCAGAGATATTCTTCCTCATTTCGGGGGCCACCCAATGGCTGCGGGGATGACGCTGAATGCCGAAGATGT
GCCGGATTTGCGGAACAGGCTGAATGAAATAGCAGACAACACGCTTACGGAAGAAGATTTTATTCCTGTACAGGAAGTCG
ATCTTGTCTGCGGTGTCGAGGACATTACAGTTGAAAGCATCACAGAAATGAACCTGCTGTCCCCATTTGGCATGCTGAAC
CCAAAACCGCACGTTCTCGTAGAAAACGCTGTGCTGGAAGATGTCCGCAAGATCGGCGCTAATAAAAATCATGTGAAAAT
GACGGTCAGAAACGAGTCGTCTCAGCTTGATTGCGTCGGTTTTAATAAAGGTGAGCTCGAAGAAGGCATTGTCCCGGGTT
CAAGAATTTCAATTGTCGGTGAGATGTCTATCAACGAATGGAACAATAGAAAAAAACCGCAGCTAATGATTAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGATGTGGGAAGACACAGTTTCCGTCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACACTTCTTCAGACTGAAGACCTTCGCCGTGAAGTGCACGTCATCA
GCTCTGAAGATCAAGCGAAGGCGTTTGACCTTGATGGTGCATATATTGTATTGCTGGATCCGCCGCCTTCGTTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGGCGCCTGAGCGGATATATTTCATTTTTCTCAATCAAGAGGACCACTTTTTATCAAC
ATTTCCTGCAAGGGACCACTTTAAGTGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTAAAAAAACACGGTT
CAGAGCTTGCGAGACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTAGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTGGTTAGCGGAGTAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

97.455

100

0.975

Multiple sequence alignment