Detailed information    

experimental Experimentally validated

Overview

Name   comA   Type   Machinery gene
Locus tag   SGL_RS14595 Genome accession   NC_000911
Coordinates   2727047..2728708 (+) Length   553 a.a.
NCBI ID   WP_010873643.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   DNA uptake   
DNA binding and uptake

Function

Competency of the natural transformation was lost in the slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 mutants and slr0197-disruptant. It was suggested that both DNA uptake for natural transformation and motility are mediated by a specific type IV-like pilus structure, while a putative DNA-binding protein encoded by slr0197 is additionally required for the DNA uptake.


Genomic Context

Location: 2722047..2733708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS20165 - 2722244..2722369 (+) 126 WP_255345203.1 hypothetical protein -
  SGL_RS14565 (slr0191) - 2722431..2724038 (+) 1608 WP_010873637.1 SpoIID/LytB domain-containing protein -
  SGL_RS14570 (slr0192) - 2724075..2724419 (+) 345 WP_010873638.1 rhodanese-like domain-containing protein -
  SGL_RS14575 (slr0193) - 2724648..2725103 (+) 456 WP_010873639.1 RNA recognition motif domain-containing protein -
  SGL_RS14580 (slr0194) rpiA 2725326..2726033 (+) 708 WP_010873640.1 ribose-5-phosphate isomerase RpiA -
  SGL_RS14585 (slr0195) - 2726089..2726640 (+) 552 WP_010873641.1 TolB family protein -
  SGL_RS14590 (slr0196) - 2726697..2727059 (+) 363 WP_223211286.1 hypothetical protein -
  SGL_RS14595 (slr0197) comA 2727047..2728708 (+) 1662 WP_010873643.1 phospholipase D-like domain-containing protein Machinery gene
  SGL_RS14600 (sll0185) - 2728812..2730071 (-) 1260 WP_010873644.1 DUF4912 domain-containing protein -
  SGL_RS14605 (slr0199) egtC 2730688..2731563 (+) 876 WP_020862095.1 ergothioneine biosynthesis protein EgtC -
  SGL_RS14610 (sll0184) sigC 2731560..2732774 (-) 1215 WP_010873646.1 RNA polymerase sigma factor SigC -

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 61256.25 Da        Isoelectric Point: 5.4178

>NTDB_id=1416 SGL_RS14595 WP_010873643.1 2727047..2728708(+) (comA) [Synechocystis sp. PCC 6803]
MGKLNRGWKPNPVVIFVALALLVAIAVIGIGKVDPLGLGQSLARPDPLPQQEEIQVYFNLNNAKGADFIDPDRDFKRQGD
NFEQIILEQIDQAQSTIDLAVQELRLPRIAQALVEKQKTGVRVRLVLENTYNQTIKEAVETEKDPFRYRGPLAYLDQNQD
GRISPAESAERDAIEILRTQKIPLIDDTEDGSKGSGLMHHKFMVLDNKTVIVTSANFTPSDQHGDYDDNFTRGNANNLLV
VQSPAVAHLFTEEFNLLWGDGPGAEKDSLFGINKPSRPAKVLKVGETTVTVKFSPDRRAIPFPNTSNGLIAQYLNQAQQK
IQLALFVFSEQALSNVINQRFQAGTEVKALIDRGFAFRYYSEGLDLLGVTLLEDCLEQPGNSPWALPTEFVGTPALPRGD
KLHHKFALVDDDTVITGSHNWSPAANHNNDETVLVLQNAQIAAHYQRELDRLYAVTEYGLPPTVQARIDRQEQECANPRP
SPKPLAKATPNRLVNLNRGSQEELESLPGIGPSTAEKIISTREQKAFTSLEDLGRVKGIGPSKIEGLRGKVTW

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=1416 SGL_RS14595 WP_010873643.1 2727047..2728708(+) (comA) [Synechocystis sp. PCC 6803]
ATGGGAAAACTAAACAGAGGCTGGAAACCAAACCCCGTTGTCATTTTTGTTGCCCTGGCCCTACTAGTGGCGATCGCCGT
CATTGGCATTGGCAAAGTTGATCCATTGGGGTTAGGACAATCCCTGGCCCGACCAGATCCTTTACCACAACAAGAGGAGA
TCCAGGTTTACTTTAACCTCAACAATGCCAAGGGAGCCGATTTCATTGACCCAGACCGCGACTTTAAACGTCAGGGGGAC
AATTTTGAGCAGATAATTCTGGAGCAAATTGACCAAGCCCAGTCCACCATTGACCTAGCAGTACAGGAATTACGCTTACC
TCGCATTGCCCAGGCATTGGTCGAGAAACAAAAGACCGGGGTCCGGGTCAGATTGGTGCTGGAAAATACCTATAACCAAA
CCATCAAAGAAGCAGTAGAAACAGAAAAGGATCCCTTCCGTTACCGTGGTCCCCTAGCCTATTTAGATCAAAATCAAGAC
GGTCGCATTAGTCCCGCAGAAAGCGCAGAACGGGATGCCATTGAAATTCTCCGCACTCAGAAAATTCCCCTCATTGACGA
CACAGAAGATGGCAGTAAAGGCTCAGGCTTAATGCACCATAAATTTATGGTCCTGGATAATAAAACGGTCATTGTAACTT
CTGCTAACTTTACCCCTAGCGACCAGCATGGCGACTATGATGACAATTTTACTCGAGGCAACGCCAATAACCTATTGGTG
GTCCAATCCCCCGCTGTCGCCCATCTTTTCACAGAGGAATTTAATCTACTTTGGGGGGACGGGCCTGGAGCAGAAAAAGA
CAGTCTCTTTGGTATTAATAAGCCCAGCAGACCAGCTAAAGTGCTAAAAGTAGGAGAGACAACGGTAACAGTAAAGTTTT
CCCCCGATCGCCGAGCAATCCCTTTTCCCAACACCAGTAATGGTTTAATTGCCCAATACCTCAATCAAGCCCAACAAAAA
ATTCAATTAGCATTGTTTGTTTTTAGTGAACAAGCATTATCTAATGTGATCAACCAAAGATTTCAAGCGGGCACGGAGGT
TAAAGCTTTAATTGATCGGGGATTTGCCTTTCGTTACTACAGCGAAGGGCTGGATCTATTGGGAGTAACTTTGCTAGAAG
ACTGCCTAGAACAACCTGGTAATAGTCCCTGGGCTTTGCCAACTGAATTTGTCGGTACCCCGGCCCTACCAAGGGGAGAT
AAATTACACCATAAGTTTGCCCTGGTGGATGATGACACCGTGATCACCGGCTCCCACAATTGGTCCCCCGCCGCTAACCA
CAACAACGATGAAACGGTATTGGTTTTACAGAATGCCCAAATAGCAGCCCATTACCAACGGGAATTAGACCGACTTTATG
CCGTCACAGAATATGGTTTACCTCCCACAGTACAAGCCCGCATCGATCGCCAGGAGCAGGAGTGCGCCAATCCCCGACCT
AGCCCTAAGCCCCTGGCAAAAGCAACTCCCAATCGTTTGGTGAATTTGAACCGAGGGAGTCAAGAGGAACTGGAAAGTTT
ACCCGGCATTGGCCCCAGCACCGCCGAAAAAATCATCTCCACCAGGGAGCAAAAGGCTTTCACTTCCCTAGAAGACTTGG
GCCGGGTCAAAGGCATTGGTCCCAGCAAAATCGAAGGTTTACGGGGCAAAGTTACCTGGTAA

Domains

Predicted by InterproScan.

PLD-like domain (PLDc_2)

(86-258)

PLD-like domain (PLDc_2)

(310-452)

Helix-hairpin-helix motif (HHH_3)

(492-552)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] S Yoshihara et al. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 42(1):63-73. [PMID: 11158445]