Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   UP12_RS12085 Genome accession   NZ_CP011007
Coordinates   2421006..2423330 (-) Length   774 a.a.
NCBI ID   WP_061408786.1    Uniprot ID   -
Organism   Bacillus pumilus strain SH-B9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2416006..2428330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UP12_RS12070 (UP12_12075) dtd 2417655..2418098 (-) 444 WP_003216527.1 D-aminoacyl-tRNA deacylase -
  UP12_RS12075 (UP12_12080) - 2418117..2420327 (-) 2211 WP_012010732.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  UP12_RS12080 (UP12_12085) - 2420470..2420982 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  UP12_RS12085 (UP12_12090) recJ 2421006..2423330 (-) 2325 WP_061408786.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  UP12_RS12090 (UP12_12095) - 2423412..2423765 (-) 354 WP_061408789.1 lipopolysaccharide assembly LapA domain-containing protein -
  UP12_RS12095 (UP12_12100) - 2423867..2424355 (-) 489 WP_058014558.1 cation:proton antiporter regulatory subunit -
  UP12_RS12100 (UP12_12105) secDF 2424542..2426770 (-) 2229 WP_058014557.1 protein translocase subunit SecDF -
  UP12_RS12105 (UP12_12110) - 2426812..2427108 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87234.92 Da        Isoelectric Point: 4.8372

>NTDB_id=141339 UP12_RS12085 WP_061408786.1 2421006..2423330(-) (recJ) [Bacillus pumilus strain SH-B9]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEQQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPAQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEEKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDARLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEIVTGSLISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKEEVSIPIHHVHAENVSTLDVEGKYVVFADVPADEQLLRQLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=141339 UP12_RS12085 WP_061408786.1 2421006..2423330(-) (recJ) [Bacillus pumilus strain SH-B9]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCTGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCAATCGAACAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTACGGGCCTAATGAACAGGCTTTTCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACCGTCGATACAGGCATTGCAGCTGTAAATGAAGCGCGTATTGCAAAAGAAATA
GGGTTAGACTTGATCATTACAGATCACCATGAACCAAGCGAAGAGCTGCCTGAAGCACTTGCGATTGTCCATCCTAAGCA
GCCAGGCTGTGAGTATCCCTTTAAAGAACTTGCCGGTGTAGGCGTAGCATTTAAAGTGGCTCATGCTCTTCTTGGTAAGC
TGCCAGCTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTCGGCAGTCGAATAGACCGGGACTGAAGGCGTTATTGAAAGAAGCAGGTGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CAGCTGTTCATCTTCTCATGACAGAGGAGAAGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCCATTGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTTGGAATTGTTGCCTCTAAATTGACGGATACATTTTCTCGTCCGGCAA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGTTCAGCACGGAGTATTCCCGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGCGACATTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGCATGACACTTCGAGCGGATGATGT
CCCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAGGAAGACTTTATTCCGATTCAACGGGTCG
ATGCTGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGCGAGACTTGAAGAAATCCGAACCATTGGAGCAAATCAAAATCATATCAAAAT
GTCTCTGAAAGATGAAGACAAACTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTGAAAGAAGAAATTGTCACAGGTA
GCCTGATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCACAGCTCATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGACCCCGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAAGAGGAAGTCAGCATCCCGATTCATCATGTCCATGCAGAAAATGTTT
CAACACTCGATGTTGAAGGCAAGTATGTCGTCTTTGCAGATGTCCCTGCAGATGAACAGTTGCTAAGGCAGCTGTTAGAA
AATCAAAAGCCAGCTAGAGTGTATACAGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCTCTCAAGGAACAAGGAATGGAGCTTGCGAAACATA
GAGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(309-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.674

Multiple sequence alignment