Detailed information NTG0139579

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: AS852_RS06115
• Genome accession: NZ_CP013218
• Coordinates: 1206894..1209302 (+)
• Length: 802 a.a.
• NCBI ID: WP_000560853.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain LA-MRSA ST398 isolate E154
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1201894..1214302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS852_RS06090 (AS852_05825)	-	1202002..1202403 (+)	402	WP_000354182.1	VOC family protein	-
AS852_RS06095 (AS852_05830)	-	1202666..1204363 (-)	1698	WP_000312759.1	Rqc2 family fibronectin-binding protein	-
AS852_RS06100 (AS852_05835)	gmk	1204638..1205261 (+)	624	WP_000368225.1	guanylate kinase	-
AS852_RS06105 (AS852_05840)	rpoZ	1205261..1205479 (+)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
AS852_RS06110 (AS852_05845)	coaBC	1205695..1206894 (+)	1200	WP_000722168.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
AS852_RS06115 (AS852_05850)	priA	1206894..1209302 (+)	2409	WP_000560853.1	primosomal protein N'	Machinery gene
AS852_RS06120 (AS852_05855)	-	1209805..1210776 (+)	972	WP_000751710.1	hypothetical protein	-
AS852_RS06125 (AS852_05860)	-	1210914..1211117 (-)	204	WP_001198022.1	TM2 domain-containing protein	-
AS852_RS06130 (AS852_05865)	-	1211416..1211904 (+)	489	WP_000985287.1	peptide deformylase	-
AS852_RS06135 (AS852_05870)	fmt	1211897..1212832 (+)	936	WP_000161301.1	methionyl-tRNA formyltransferase	-
AS852_RS06140 (AS852_05875)	rsmB	1212829..1214136 (+)	1308	WP_000572239.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92535.44 Da
• Isoelectric Point: 6.7334

>NTDB_id=139579 AS852_RS06115 WP_000560853.1 1206894..1209302(+) (priA) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKED
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQFKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRNGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=139579 AS852_RS06115 WP_000560853.1 1206894..1209302(+) (priA) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCCGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTAATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCAGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGTTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGGTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTACTTATGAAGTTGTTAAAAGAGGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACTAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAATTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATTTAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATAAGGAATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGATTAATCATTATTGATGAAGAACATGAATCTACATATAAACAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGTGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATCGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGGGATTGTGGATATGTACCGCAATGCCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAACATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAATTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.433	100	0.509