Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   AS52_RS22610 Genome accession   NZ_CP010586
Coordinates   4479415..4481757 (-) Length   780 a.a.
NCBI ID   WP_049166221.1    Uniprot ID   A0A806TWA0
Organism   Priestia megaterium Q3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4474415..4486757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS52_RS22585 (AS52_04667) - 4474808..4474984 (-) 177 WP_013059305.1 hypothetical protein -
  AS52_RS22590 (AS52_04668) - 4475151..4476035 (+) 885 WP_223546263.1 N-acetylmuramoyl-L-alanine amidase -
  AS52_RS22595 (AS52_04669) dtd 4476026..4476487 (-) 462 WP_028411689.1 D-aminoacyl-tRNA deacylase -
  AS52_RS22600 (AS52_04670) - 4476503..4478701 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  AS52_RS22605 (AS52_04671) - 4478887..4479399 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  AS52_RS22610 (AS52_04672) recJ 4479415..4481757 (-) 2343 WP_049166221.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  AS52_RS22615 (AS52_04673) secDF 4481899..4484136 (-) 2238 WP_049166222.1 protein translocase subunit SecDF -
  AS52_RS22620 (AS52_04674) - 4484274..4484567 (-) 294 WP_028411692.1 post-transcriptional regulator -
  AS52_RS22625 (AS52_04675) spoVB 4484632..4486242 (+) 1611 WP_080974290.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87860.45 Da        Isoelectric Point: 4.7998

>NTDB_id=139450 AS52_RS22610 WP_049166221.1 4479415..4481757(-) (recJ) [Priestia megaterium Q3]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLGYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSVDSENHIAKGSARSIQGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSEDVEELRRRLNEQASNVLTQQDFIPITSVDTVCKTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFEQVFTDQMKVVAFHDSTIDKYKSMLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPSQ
KWLEALFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSTKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=139450 AS52_RS22610 WP_049166221.1 4479415..4481757(-) (recJ) [Priestia megaterium Q3]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCAGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACTGATCACCACGAGCCGGGTCCGGTTTTACCCGATGCGTATGCAATTATTCATCCAAAGCT
TCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGGCTATCTCAAGCTCACGAAGCGTACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCCATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCATTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAATGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAGTATGTATCCTCCGGATGAAAATAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTTGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTATTAAGTGTTGACTCTGAAAACCATATTGCTAAAGGTTCAGCAAGAAGTATCCAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCTGGCATGACGTTAAACAGCGA
GGATGTAGAAGAACTGCGCAGACGCCTTAATGAACAAGCAAGCAACGTGTTAACTCAACAGGACTTTATTCCAATTACAT
CAGTTGATACAGTATGCAAAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGCGCGCTTGCTCCATTTGGTATG
CATAATCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGCGCGAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAGGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGATGAGTGGCAGATGTTTGATTATCGCAGCGCGGTTGATGTCAAACGTTTCTTTGAACAGGTATTCAC
AGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGATGCTGCCTATGGAACATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGCATGTTTGCAGGAGAACAGATCGTTTTATTAGATTTGCCTCCGTCACAG
AAGTGGCTTGAAGCTTTGTTTGTCCAAGGTATTCCTTCTAGGATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTCTACGGCTTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCAATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTACGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A806TWA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.385

100

0.554

Multiple sequence alignment