Detailed information NTG0137977

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: SAST43_RS05685
• Genome accession: NZ_CP012974
• Coordinates: 1151059..1153467 (+)
• Length: 802 a.a.
• NCBI ID: WP_062909778.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain ST20130943
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1146059..1158467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAST43_RS05660 (SAST43_01093)	-	1146167..1146568 (+)	402	WP_000354184.1	VOC family protein	-
SAST43_RS05665 (SAST43_01095)	-	1146831..1148528 (-)	1698	WP_000312772.1	NFACT family protein	-
SAST43_RS05670 (SAST43_01096)	gmk	1148803..1149426 (+)	624	WP_000368227.1	guanylate kinase	-
SAST43_RS05675 (SAST43_01097)	rpoZ	1149426..1149644 (+)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
SAST43_RS05680 (SAST43_01098)	coaBC	1149860..1151059 (+)	1200	WP_062909777.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
SAST43_RS05685 (SAST43_01099)	priA	1151059..1153467 (+)	2409	WP_062909778.1	primosomal protein N'	Machinery gene
SAST43_RS05690 (SAST43_01101)	-	1153973..1154926 (+)	954	WP_000751702.1	hypothetical protein	-
SAST43_RS05695 (SAST43_03243)	-	1155089..1155292 (-)	204	WP_001198023.1	TM2 domain-containing protein	-
SAST43_RS05700 (SAST43_01103)	-	1155591..1156079 (+)	489	WP_000985290.1	peptide deformylase	-
SAST43_RS05705 (SAST43_03244)	fmt	1156072..1157007 (+)	936	WP_000161291.1	methionyl-tRNA formyltransferase	-
SAST43_RS05710 (SAST43_03245)	rsmB	1157004..1158311 (+)	1308	WP_000572225.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92490.51 Da
• Isoelectric Point: 6.9802

>NTDB_id=137977 SAST43_RS05685 WP_062909778.1 1151059..1153467(+) (priA) [Staphylococcus aureus strain ST20130943]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYCYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLNRKEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMKYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=137977 SAST43_RS05685 WP_062909778.1 1151059..1153467(+) (priA) [Staphylococcus aureus strain ST20130943]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCTGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTGATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCTGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGCTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGCTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTGTTATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACCAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATATAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATTAGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAGCAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGCGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATAGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAAATCGAAAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCGTTTATGTTATG
TCGGGATTGCGGATATGTACCGCAATGTCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGA
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAGCATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAGTTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.556	100	0.51